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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 114,222 results for: MPI

Showing: 6381 - 6400 of 114222 results

  1. pHH0103 NEDD4L WW domain #2

    Plasmid
    #104205
    Purpose
    Bacterial expression of WW domain #2 from NEDD4L
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    NEDD4L WW domain #2 (NEDD4L Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104205-plasmid-map-sequence-id-203673

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  2. pGAL-flp(H305L)-NAT

    Plasmid
    #160380
    Purpose
    overexpression of Flp recombinase step-arrest mutant in yeast
    Depositor
    John McCusker
    Insert
    FLP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    H305L
    Promoter
    GAL
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    160380-plasmid-map-sequence-id-314503

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  4. pUA97

    Plasmid
    #69340
    Purpose
    It encodes a fluorescent protein that can be fused to a gene of interest using SEC CRISPR strategy
    Depositor
    Oliver Hobert
    Insert
    ceBFP SEC 3xFlag
    Use
    Tags
    3xFlag and ceBFP (mTagBFP)
    Expression
    Worm
    Mutation
    sqt-1(e1350)
    Promoter
    Available since
    Dec. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    69340-plasmid-map-sequence-id-133241

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  5. pAG416GAL-EMCV_3C

    Plasmid
    #201941
    Purpose
    Expresses EMCV 3C protease from a GAL promoter with a URA3 marker
    Depositor
    Alejandro Chavez
    Insert
    EMCV 3C protease (EMCVgp1 )
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    Aug. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    201941-plasmid-map-sequence-id-403575

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  6. NM-01

    Plasmid
    #69799
    Purpose
    Bacterial expression of Cas9 with His, MBP, and Strep tags
    Depositor
    Gerard Cagney
    Insert
    Cas9
    Use
    Tags
    HIS, MBP, and StrepII
    Expression
    Bacterial
    Mutation
    Aspartate 10 to Alanine (D10A) and Histidine 840 …
    Promoter
    Available since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. pDTA-5

    Plasmid
    #128845
    Purpose
    Gateway-compatible p15A origin plasmid with a DTA negative selection cassette for targeting vector construction, I-CeuI, low copy number
    Depositor
    Mario Capecchi
    Type
    Empty backbone
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    128845-plasmid-map-sequence-id-252002

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  9. pJK633

    Plasmid
    #72397
    Purpose
    Produces Escherichia coli aminoimidazole ribonucleotide synthetase, N-terminal His6 tag (H6EcPurM)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole synthetase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    72397-plasmid-map-sequence-id-134629

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  10. TetCMV-Pfv-Sapphire

    Plasmid
    #203650
    Purpose
    Mammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the doxycycline-inducible promoter.
    Depositor
    Liam Holt
    Article
    Shu T et et al bioRxiv 2022
    Insert
    PfV
    Use
    Lentiviral
    Tags
    Sapphire
    Expression
    Mutation
    Promoter
    Available since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    203650-plasmid-map-sequence-id-402480

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  11. pHH0103 NEDD4 WW domain #1

    Plasmid
    #104208
    Purpose
    Bacterial expression of WW domain #1 from NEDD4
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    NEDD4 WW domain #1 (NEDD4 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Feb. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    104208-plasmid-map-sequence-id-204367

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  13. BII-gR-PnTW

    Plasmid
    #133356
    Purpose
    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications) coexpressed with tdTomato-NLS
    Depositor
    Blair Madison
    Insert
    tdTomato-NLS
    Use
    Tags
    HA-tagged tdTomato
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available since
    Jan. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133356-plasmid-map-sequence-id-267393

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  14. pLinker-BirA-3xHA-HXGPRT-loxP

    Plasmid
    #86668
    Purpose
    for generation of BirA tagged strains using CRISPR tagging technology
    Depositor
    David Sibley
    Insert
    Linker-BirA-3xHA-HXGPRT 3'UTR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    86668-plasmid-map-sequence-id-190141

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  15. pAS1-rab-3P::his-24::CyOFP

    Plasmid
    #124347
    Purpose
    Nuclear Localized Pan-Neuronal CyOFP
    Depositor
    Andrew Leifer
    Insert
    rab-3, CyOFP (rab-3 Nematode)
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    April 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    124347-plasmid-map-sequence-id-241978

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  17. pKSB- 3xP3-GFPsv40 GGGG AAGA

    Plasmid
    #174531
    Purpose
    3xP3-GFP fluorescence marker cassette for gene knock-in in mosquitoes
    Depositor
    Eric Marois
    Insert
    3xP3-GFP BsaI cassette
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    3xP3
    Available since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    174531-plasmid-map-sequence-id-343226

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  19. pGEX-MK2

    Plasmid
    #30187
    Depositor
    Bruce Tempel
    Insert
    MK2 (Kcna2 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 17, 2011
    Availability
    Academic Institutions and Nonprofits only

  20. pE-SUMO CRYGD

    Plasmid
    #80755
    Purpose
    Expression of human gamma D-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYGD (CRYGD Human)
    Use
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80755-plasmid-map-sequence-id-153570

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Showing: 6381 - 6400 of 114222 results