We narrowed to 22,696 results for: Sis;

1. pYea5.1_C99_sfGFP

   Plasmid

   #207802

   Purpose

   Yeast 2µ vector for expression of Suc2SP-C99-sfGFP-GAL4

   Depositor

   Dieter Langosch

   Article

   Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

   Insert

   Suc2SP-C100-sfGFP-GAL4 fusion protein
   

   Use

   Tags

   Strep-TagII

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYea5.1_Notch3_sfGFP

   Plasmid

   #207864

   Purpose

   Yeast 2µ vector for expression of Suc2SP-Notch3_100-sfGFP-GAL4

   Depositor

   Dieter Langosch

   Article

   Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

   Insert

   Suc2SP-Notch3_100-sfGFP-GAL4 fusion protein
   

   Use

   Tags

   Strep-TagII

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pACBE-FSR (pXK-1648)

   Plasmid

   #208821

   Purpose

   Universal Fluorescent Surrogate Reporter for adenine and cytosine base editors. Key Construct: CMV-mCherry-CMV-eGFP* (ATAACG)-hU6-gRNA.

   Depositor

   Kun Xu

   Article

   Ma et al J Biol Chem. 2022 Jul;298(7):102103. doi

   Type

   Empty backbone

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, U6

   Available Since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB-scrCtnnd2.1-mCherry-CAAX

   Plasmid

   #209108

   Purpose

   Membrane tethered mCherry scramble of shRNA targeting Ctnnd2 N-terminus

   Depositor

   Cagla Eroglu

   Article

   Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

   Insert

   scrCtnnd2.1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB-shCtnnd2.1-mCherry-CAAX

   Plasmid

   #209107

   Purpose

   Membrane tethered mCherry shRNA targeting Ctnnd2 N-terminus

   Depositor

   Cagla Eroglu

   Article

   Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

   Insert

   shCtnnd2.1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO.1-shCdh20.1 GFP

   Plasmid

   #209105

   Purpose

   GFP expressing shRNA targeting Cdh20

   Depositor

   Cagla Eroglu

   Article

   Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

   Insert

   shCdh20.1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO.1-shCdh10.1 GFP

   Plasmid

   #209101

   Purpose

   GFP expressing shRNA targeting Cdh10

   Depositor

   Cagla Eroglu

   Article

   Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

   Insert

   shCdh10.1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1-shCtnnd2.2-GFP

   Plasmid

   #209097

   Purpose

   GFP expressing shRNA targeting Ctnnd2 3' UTR

   Depositor

   Cagla Eroglu

   Article

   Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

   Insert

   shCtnnd2.2
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO.1-scrCtnnd2.1-GFP

   Plasmid

   #209096

   Purpose

   GFP expressing scramble of shRNA targeting Ctnnd2 N terminus

   Depositor

   Cagla Eroglu

   Article

   Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

   Insert

   scrCtnnd2.1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRH2961

    Plasmid

    #210144

    Purpose

    Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::LYS1-GFP::tADH1

    Depositor

    Randy Hampton

    Article

    Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

    Insert

    YIp ADE2-LEU2 pTDH3::LYS1-GFP::tADH1 (LYS1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKE011

    Plasmid

    #207912

    Purpose

    Level 0 for gRNA in C4 module (5' GCAG-3' TCAG)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    pU3-AarI-scaffold-term
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS018

    Plasmid

    #207911

    Purpose

    Level 0 for gRNA in C3 module (5' TTCG-3' GCAG)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    pU6-AarI-scaffold-term
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS017

    Plasmid

    #207909

    Purpose

    Level 0 for gRNA in C1 module (5' GGCT-3' AGCC)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    pU6-AarI-scaffold-term
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS020

    Plasmid

    #207915

    Purpose

    Level 0 for gRNA in D3 module (5' TCCC-3' CTGC)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    pU6-AarI-scaffold-term
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKE012

    Plasmid

    #207914

    Purpose

    Level 0 for gRNA in AD2 module (5' TGAC-3' TCCC)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    pU3-AarI-scaffold-term
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKE010

    Plasmid

    #207910

    Purpose

    Level 0 for gRNA in C2 module (5' AGCC-3' TTCG)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    pU3-AarI-scaffold-term
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCS019

    Plasmid

    #207913

    Purpose

    Level 0 for gRNA in D1 module (5' TCAG-3' TGAC)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    pU6-AarI-scaffold-term
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Dox-SOScat

    Plasmid

    #207329

    Purpose

    PiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized catalytic domain of SOS (SOScat)

    Depositor

    Jared Toettcher

    Article

    Underhill et al Development. 2023 Aug 15;150(16):dev2016

    Insert

    son of sevenless homolog 2 variant, catalytic domain
    

    Use

    Tags

    myristoylation tag - BFP

    Expression

    Bacterial

    Mutation

    range: 593 to 1078

    Promoter

    Tet Response Element

    Available Since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-C1-hEFA6C

    Plasmid

    #206414

    Purpose

    Mammalian expression of hEFA6C

    Depositor

    Kazuhisa Nakayama, Hye-Won Shin

    Article

    Ueda et al FEBS Lett. 2013 Jun 5;587(11):1617-23. d

    Insert

    EFA6C (PSD2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZCS41 (U6p::GCGAAGTGACGGTAGACCGT)

    Plasmid

    #193050

    Purpose

    Encodes guide RNA expression targeting PX740 landing pad

    Depositor

    Patrick Phillips

    Article

    Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

    Insert

    Guide RNA
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available Since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pcDNA3.1 NKX6-2-Venus S246D

    Plasmid

    #203716

    Purpose

    human NKX6-2 S246D phosphomimic mutant

    Depositor

    Federico Herrera

    Article

    Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023

    Insert

    NKX6-2 (S246D) fused with Venus (NKX6-2 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    NKX6-2 (S246D)

    Promoter

    CMV

    Available Since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcDNA3.1 NKX6-2-Venus T252D

    Plasmid

    #203718

    Purpose

    human NKX6-2 T252D phosphomimic mutant

    Depositor

    Federico Herrera

    Article

    Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023

    Insert

    NKX6-2 (T252D) fused with Venus (NKX6-2 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    NKX6-2 (S252D)

    Promoter

    CMV

    Available Since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1 NKX6-2-Venus

    Plasmid

    #203712

    Purpose

    Constitutive mammaliam expression of human NKX6-2 cDNA fused with the Venus fluorescent protein

    Depositor

    Federico Herrera

    Article

    Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023

    Insert

    NKX6-2 fused with Venus (NKX6-2 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTet-Bxb1-Xis-mScarlet

    Plasmid

    #195570

    Purpose

    Expresses Bxb1 excisionase fused with mScarlet for fluorescence

    Depositor

    Richard Murray

    Article

    Pandey et al ACS Synth Biol. 2023 Feb 17;12(2):511-52

    Insert

    Bxb1 Excisionase Fused With mScarlet
    

    Use

    Synthetic Biology

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    P7

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. NK809

    Plasmid

    #196406

    Purpose

    Strong expression of firefly and nanoluc luciferases codon-optimized for S. rosetta

    Depositor

    Nicole King

    Article

    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

    Insert

    Sro5EFL-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    N/A (synthetic)

    Promoter

    Available Since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. NK810

    Plasmid

    #196407

    Purpose

    Nanoluc luciferase under S. rosetta SPAG6 promoter with internal actin-firefly control

    Depositor

    Nicole King

    Article

    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

    Insert

    Sro5spag6-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    N/A (synthetic)

    Promoter

    Available Since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. NK811

    Plasmid

    #196408

    Purpose

    Nanoluc luciferase under S. rosetta SPAG6 promoter with mutated RFX binding site with internal actin-firefly control

    Depositor

    Nicole King

    Article

    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

    Insert

    Sro5spag6_mut-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    N/A (synthetic)

    Promoter

    Available Since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCA528 AMSHLP 16-218

    Plasmid

    #180609

    Purpose

    Bacterial expression for AMSHLP MIT domain residues 16-218. Internal ID: WISP20-83

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    AMSHLP residues 16-218
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 16-218

    Promoter

    T7

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. TEG +Q111T

    Plasmid

    #187435

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1
    ATP1B1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    changed glutamine at 117 to threonine

    Promoter

    PH and p10

    Available Since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSF-NgPglO

    Plasmid

    #198204

    Purpose

    pSN18 derivative encoding N. gonorrhoeae PglO with C-terminal FLAG epitope tag

    Depositor

    Michael Jewett

    Article

    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

    Insert

    NgPglO
    

    Use

    Tags

    Flag tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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