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MSP969

Plasmid

#98802

Purpose

Expression Vector

Depositor

David Liu

Article

Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

Insert

pET28 MBP-ENLYFHS-TEV T146S, D148P, S153N, S170A, N177M

Expression

Bacterial

Mutation

T146S, D148P, S153N, S170A, N177M

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pU6-Mb-crRNA

Plasmid

#78959

Purpose

Cloning vector for expression of MbCpf1 crRNA. It contains BsmB1 site for cloning.

Depositor

Jin-Soo Kim

Article

Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

June 17, 2016

Availability

Academic Institutions and Nonprofits only

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pET11_DHNTPase

Plasmid

#73154

Purpose

Expresses in E. coli, E. coli Dihydroneopterin triphosphate pyrophosphatase

Depositor

Maurice Bessman, Sandra Gabelli

Article

Gabelli et al Structure. 2007 Aug;15(8):1014-22.

Insert

ORF17_DHNTPase_NUD

Expression

Bacterial

Available Since

March 3, 2016

Availability

Academic Institutions and Nonprofits only
pMAL-c5X-SPR16

Plasmid

#67001

Purpose

Expresses malE-SPR16 in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-SPR16

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-AB12

Plasmid

#67005

Purpose

Expresses malE-AB12 in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-AB12

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-BQ_6

Plasmid

#67006

Purpose

Expresses malE-BQ_6 in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-BQ_6

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pSALECT-PRAI

Plasmid

#59709

Purpose

pSALECT with PRAI insert

Depositor

Christopher Anderson

Article

Hsiau et al PLoS One. 2015 Mar 19;10(3):e0119927. do

Insert

PRAI

Expression

Bacterial

Available Since

Sept. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pGGI000

Plasmid

#48863

Purpose

Empty entry vector for creating GreenGate N-terminal tag + coding sequence + C-terminal tag modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGD006

Plasmid

#48836

Purpose

Provides the SV40 nuclear localization signal as GreenGate C-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

SV40 NLS

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGB006

Plasmid

#48823

Purpose

Provides an ER signal sequence as GreenGate N-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

signal sequence ER

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGC014

Plasmid

#48827

Purpose

Provides GFP as GreenGate coding sequence module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

GFP

Use

Golden gate compatible cloning vector

Mutation

A206K

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGST2-yVps23-UEV_C133A

Plasmid

#45924

Depositor

James Hurley

Article

Ren et al EMBO J. 2011 Jun 1;30(11):2130-9. doi: 1

Insert

yeast Vps23-UEV(1-160) (STP22 Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

C133A

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pEG795

Plasmid

#40122

Depositor

Geraldine Seydoux

Article

Griffin et al Cell. 2011 Sep 16;146(6):955-68.

Insert

Mex-5 (ATG through 648 bp of 3'UTR) (RNAi resistant exon 2) (mex-5 Nematode)

Use

Gateway donor vector

Mutation

Exon 2 recoded to be RNAi-resistant

Available Since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
pDEST17-1ALigN

Plasmid

#20024

Depositor

Oscar Millet

Article

Tadeo et al PLoS Biol. 2009 Dec;7(12):e1000257. doi:

Insert

1A Domain of DNA Ligase N

Tags

His tag

Expression

Bacterial

Available Since

Jan. 13, 2009

Availability

Academic Institutions and Nonprofits only
pGST2-Ub CPS (8-16)

Plasmid

#17631

Depositor

James Hurley

Article

Lee et al Nat Struct Mol Biol. 2006 Mar . 13(3):26

Insert

Ub CPS (8-16) (RPL40A Budding Yeast)

Tags

GST

Expression

Bacterial

Available Since

March 28, 2008

Availability

Academic Institutions and Nonprofits only
RCAS(A) Chfz-7 WT (CT#532)

Plasmid

#13929

Depositor

Cliff Tabin

Article

Hartmann et al Development. 2000 Jul . 127(14):3141-59.

Insert

Fz 7 (FZD7 Chicken)

Use

Retroviral; Avian expression

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pET21a(+)-PA PdxB

Plasmid

#12668

Depositor

SeWon Suh

Article

Ha et al Acta Crystallograph Sect F Struct Biol C

Insert

Erythronate-4-phosphate dehydrogenase

Tags

His

Expression

Bacterial

Available Since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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7861 pRS305-pUG72-HSC82out

Plasmid

#10899

Depositor

Susan Lindquist

Article

Cowen et al Science. 2005 Sep 30. 309(5744):2185-9.

Insert

HSC82 URA3 (HSC82 Budding Yeast)

Use

Cre/Lox

Expression

Yeast

Available Since

April 26, 2006

Availability

Academic Institutions and Nonprofits only

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C119

Plasmid

#174354

Purpose

CRISPR-Cas9 with guide targeting human PABPC1 genomic location near STOP codon

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

gRNA sequence targeting human PABPC1 near stop codon region

Use

CRISPR

Availability

Academic Institutions and Nonprofits only

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pRL055 pDESTattB_cryaa:Venus

Plasmid

#213509

Purpose

attB-containing pDEST plasmid, for use in Multisite Gateway reaction

Depositor

Christian Mosimann

Article

Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

Type

Empty backbone

Use

Zebrafish expression

Available Since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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