We narrowed to 6,946 results for: crispr cas9 plasmids

1. PLJR965_Rv1420

   Plasmid

   #241229

   Purpose

   CRISPRi gene silencing plasmid with sgRNA (Rv1420) dCas9 TetR and KanR L5 Int attP for M. tuberculosis

   Depositor

   Kyu Rhee

   Insert

   Rv1420 sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Available since

   Aug. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. miniCGBE1-SpRY (pBM1571)

   Plasmid

   #170105

   Purpose

   CMV promoter expression plasmid for rAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   rAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1 and SpRY-nCas9 (D10A/A61R/L1111R…

   Promoter

   CMV

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEL848

   Plasmid

   #214238

   Purpose

   FrCas9 cytosine base editing plasmid V2.1 in rice

   Depositor

   Yong Zhang

   Article

   He et al Plant Biotechnol J. 2024 Sep;22(9):2488-

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYDE009

   Plasmid

   #194151

   Purpose

   Delivery of dcas9 into A. baumannii via Tn7 for CRISPRi. Tn7 element has tetR-tetP-dcas9, GmR; plasmid backbone is CarbR.

   Depositor

   Edward Geisinger

   Article

   Bai et al J Bacteriol. 2021 May 20;203(12):e005652

   Insert

   tetR-tetP-dcas9-rrnB T1-T7Te
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tetp

   Available since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNuc-cis

   Plasmid

   #202796

   Purpose

   RK2-based conjugative plasmid containing the TevSpCas9 dual-endonuclease for targeted bacterial killing

   Depositor

   David Edgell

   Article

   Hamilton et al Nat Commun. 2019 Oct 4;10(1):4544. doi:

   Insert

   TevSpCas9, sgRNA cassette, oriT, CenArsHis
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330 p53

   Plasmid

   #59910

   Purpose

   pX330 backbone expressing sgRNA targeting p53 to edit mouse p53. Expresses Cas9 from CBh promoter

   Depositor

   Tyler Jacks

   Article

   Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

   Insert

   sgRNA targeting p53 (Trp53 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330 Pten

   Plasmid

   #59909

   Purpose

   pX330 backbone expressing sgRNA targeting Pten to edit mouse Pten. Expresses Cas9 from CBh promoter

   Depositor

   Tyler Jacks

   Article

   Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

   Insert

   sgRNA targeting Pten (Pten Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330 Ctnnb1.1

   Plasmid

   #59911

   Purpose

   pX330 backbone expressing sgRNA targeting Ctnnb1 to edit mouse beta-Catenin. Expresses Cas9 from CBh promoter

   Depositor

   Tyler Jacks

   Article

   Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

   Insert

   sgRNA targeting Ctnnb1 (Ctnnb1 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-PITCh-H3C2

   Plasmid

   #207780

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H3C2 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of H3C2 (H3C2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-PITCh-TUBA1B

    Plasmid

    #207763

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of TUBA1B for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of TUBA1B (TUBA1B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330-CETN2

    Plasmid

    #227291

    Purpose

    Expresses SpCas9 and a sgRNA targeting the N-terminus of CETN2 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of CETN2 (CETN2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330-CENPA

    Plasmid

    #227282

    Purpose

    Expresses SpCas9 and a sgRNA targeting the N-terminus of CENPA for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of CENPA (CENPA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRISP_IS

    Plasmid

    #120425

    Purpose

    This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3 and IS5.

    Depositor

    Tamás Fehér

    Article

    Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:

    Insert

    CRISPR spacers targeting IS1, IS5, IS3
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    constitutive

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-SlugABE-NNG

    Plasmid

    #214356

    Purpose

    Expresses SlugABE-NNG

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugABE-NNG
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Q782R/S888R/L906R/N984S/E1012K/K1016I

    Promoter

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSCL.72

    Plasmid

    #184984

    Purpose

    Express Cas9/-Eco1 RT fusion, using linker1

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Cas9-fusion_linker1-Eco1RT
    

    Use

    Tags

    SV40NLS

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal1-10

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSCL.95

    Plasmid

    #184986

    Purpose

    Express Cas9/-Eco1 RT fusion, using linker2

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Cas9-fusion_linker2-Eco1RT
    

    Use

    Tags

    SV40NLS

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal1-10

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSCL.94

    Plasmid

    #184985

    Purpose

    Express -Eco1 RT/Cas9 fusion, using linker2

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1RT-fusion_linker2-Cas9
    

    Use

    Tags

    SV40NLS

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal1-10

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSCL.71

    Plasmid

    #184983

    Purpose

    Express -Eco1 RT/Cas9 fusion, using linker1

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1RT-fusion_linker1-Cas9
    

    Use

    Tags

    SV40NLS

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal1-10

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTubb3-MC

    Plasmid

    #87112

    Purpose

    minicircle parental backbone plasmid including GFP knock-in donor targeting Tubb3 gene and one cutting site for HITI

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    GFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAVS1-iPEmax-Hygro

    Plasmid

    #214020

    Purpose

    inducible PEmax system for controllable prime editing; this plasmid is used to insert PEmax prime editor in one allele of AAVS1 locus

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    PEmax
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-tight

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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