We narrowed to 10,408 results for: plasmids 101

1. pFB-CMV-DIO-MTS-roGFP-WPRE

   Plasmid

   #245375

   Purpose

   Cre-dependent expression of a green fluorescent protein biosensor to determine mitochondrial cellular reduction-oxidation (redox) states.

   Depositor

   D. James Surmeier

   Article

   Tubert et al Neurobiol Dis. 2023 Nov;188:106328. doi:

   Insert

   roGFP
   

   Use

   AAV

   Tags

   mitochondrial-targeting sequence of the S. pombe …

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZNpF-∆11cag

   Plasmid

   #245370

   Purpose

   Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

   Depositor

   Bruce Hamilton

   Article

   Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

   Insert

   Zfp423-∆11cag (Zfp423 Mouse)
   

   Use

   Luciferase and Synthetic Biology

   Tags

   NanoLuciferase

   Expression

   Mammalian

   Mutation

   11-bp deletion, H96Wfs*4, plus 3 synonymous mutat…

   Promoter

   EF1a

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZNpF-∆7cg

   Plasmid

   #245371

   Purpose

   Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

   Depositor

   Bruce Hamilton

   Article

   Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

   Insert

   Zfp423-∆7cg (Zfp423 Mouse)
   

   Use

   Luciferase and Synthetic Biology

   Tags

   NanoLuciferase

   Expression

   Mammalian

   Mutation

   7-bp deletion, H96Vfs*29, plus 2 synonymous mutat…

   Promoter

   EF1a

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUBC(k)-AT5G42080-mKOk

   Plasmid

   #224852

   Purpose

   Plasmid for expression of AT5G40280.1 coding sequence tagged with mKOk in plants

   Depositor

   Rebecca Roston

   Article

   LaBrant et al bioRxiv 2025.06.20.660776; doi: https://

   Insert

   AT5G40280.1 (ERA1 Mustard Weed)
   

   Expression

   Plant

   Available Since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCLJ-Fgf5-t2A-eGFP

   Plasmid

   #227504

   Purpose

   Inserts t2a-eGFP at the 3' end of the Fgf5 gene alsong with a removable selection cassette

   Depositor

   Joanna Wysocka

   Article

   Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

   Insert

   EGFP
   

   Use

   AAV and Cre/Lox

   Available Since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFNC-5

   Plasmid

   #233297

   Purpose

   Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. TcR, easily curable via sucrose counterselection.

   Depositor

   Pablo Ivan Nikel

   Article

   Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

   Inserts

   BxbI phage integrase
   sacB
   

   Expression

   Bacterial

   Promoter

   PEM7 and unknown

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-hU6-cs1-PuroR-T2A-BFP

   Plasmid

   #230934

   Purpose

   gRNA cloning vector with direct capture sequence 1 in stem loop 2

   Depositor

   Charles Gersbach

   Article

   Liu et al Cell Genom. 2025 Feb 4:100766. doi: 10.1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   TagBFP

   Available Since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFNC-6

   Plasmid

   #227669

   Purpose

   Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. GmR, easily curable via sucrose counterselection.

   Depositor

   Pablo Ivan Nikel

   Article

   Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

   Inserts

   BxbI phage integrase
   sacB
   

   Expression

   Bacterial

   Promoter

   PEM7

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBK2043-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9

   Plasmid

   #223163

   Purpose

   2nd gen. AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA Scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 204-310 (MECP2)

   Available Since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. FgH1tUTG-sgRNA.FOXA-ZEB2_CRISPRd

    Plasmid

    #216170

    Purpose

    Express the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experiment

    Depositor

    Makiko Iwafuchi

    Article

    Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

    Insert

    sgRNA- FOXA.bs- ZEB2.locus
    

    Use

    Lentiviral

    Tags

    EGFP

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. FgH1tUTG-sgRNA.Control-ZEB2_CRISPRd

    Plasmid

    #216172

    Purpose

    Express the gRNA targeting the control site in the human ZEB2 locus for the CRISPRd experiment

    Depositor

    Makiko Iwafuchi

    Article

    Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

    Insert

    sgRNA- Control- ZEB2.locus
    

    Use

    Lentiviral

    Tags

    EGFP

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. LRG-sgRNA.FOXA-ZEB2_CRISPRd_v2

    Plasmid

    #216173

    Purpose

    Express the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experiment

    Depositor

    Makiko Iwafuchi

    Article

    Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

    Insert

    sgRNA- FOXA.bs- ZEB2.locus
    

    Use

    Lentiviral

    Tags

    EGFP

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Ef1a-FAS-GCaMP6f-WPRE

    Plasmid

    #141237

    Purpose

    AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in mammalian cells that express Cre recombinase)

    Depositor

    Guohong Cui

    Article

    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

    Insert

    GCaMP6f
    

    Use

    AAV and Cre/Lox

    Tags

    6XHIS and Xpress

    Promoter

    Human elongation factor-1 alpha (EF-1 alpha)

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAS_4xMmaPylT(UUA)_EF1_sfGFP150TAA

    Plasmid

    #174897

    Purpose

    ochre suppression reporter sfGFP150TAA expression, with MmaPylT(UUA) ochre suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    sf GFP
    

    Expression

    Mammalian

    Mutation

    150TAA in sfGFP

    Promoter

    EF1

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAS_4xMmaPylT(UCA)_EF1_sfGFP150TGA

    Plasmid

    #174898

    Purpose

    opal suppression reporter sfGFP150TGA expression, with MmaPylT(UCA) opal suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    sfGFP
    

    Expression

    Mammalian

    Mutation

    150TGA in sfGFP

    Promoter

    EF1

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-LS

    Plasmid

    #205438

    Purpose

    Expression of Gaussia luciferase (gLuc) and spectinomycin resistance (Spec) genes

    Depositor

    Masamichi Kamihira

    Article

    Huang et al J Biosci Bioeng. 2021 Nov;132(5):469-478

    Inserts

    Gaussia luciferase gene
    spectinomycin resistance gene
    

    Use

    Cre/Lox and Luciferase; Chlamydomonas expression

    Promoter

    Heat shock protein 70A (HSP70A)/ribulose bisphosp…

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CMV-Hsp1-Hsp2Cas9-Y-P2A-puro

    Plasmid

    #192132

    Purpose

    Expresses highly specific Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

    Depositor

    Yongming Wang

    Article

    Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

    Insert

    Hsp1-Hsp2Cas9-Y
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    Hsp1-Hsp2Cas9 (Y446A)

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBABR mCherry-Bcd-LEXY

    Plasmid

    #182596

    Purpose

    Drosophila integration plasmid expressing mCherry-Bcd-LEXY fusion protein under the maternal tubulin promoter.

    Depositor

    Jared Toettcher

    Article

    Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

    Insert

    Bcd (bcd Fly)
    

    Tags

    LEXY and mCherry

    Expression

    Insect

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB-TAC-OKMS-KRAB-dCas9

    Plasmid

    #120356

    Purpose

    piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Insert

    OKMS cassette
    

    Use

    CRISPR; Piggybac transposon

    Expression

    Mammalian

    Mutation

    Klf4 [10-483]

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. GSX2-P2A-tGFP-DTA

    Plasmid

    #161750

    Purpose

    tGFP plasmid for the c-terminal targeting of human GSX2 locus

    Depositor

    Elena Cattaneo

    Article

    Besusso et al Stem Cell Res. 2020 Dec;49:102016. doi:

    Insert

    turboGFP
    

    Use

    CRISPR

    Tags

    P2A-tGFP

    Expression

    Mammalian

    Promoter

    endogenous GSX2 promoter

    Available Since

    Dec. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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