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Rap1A FLARE

Plasmid

#134926

Purpose

Rap1A biosensor

Depositor

Klaus Hahn

Article

O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

Insert

Rap1A, mCerulean3, RaIGDS RBD, YPet

Expression

Mammalian

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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Rap1B FLARE

Plasmid

#134927

Purpose

Rap1B biosensor

Depositor

Klaus Hahn

Article

O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

Insert

RaIGDS RBD, YPet, Cerulean, Rap1B

Expression

Mammalian

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pLX307-VHL-3F-BirA

Plasmid

#220147

Purpose

Expresses VHL-BirA for VHL substrate identification by E-STUB

Depositor

William Sellers

Article

Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

Insert

von Hippel-Lindau tumor suppressor (VHL Human)

Use

Lentiviral

Tags

3F-BirA

Expression

Mammalian

Mutation

codon optimized

Promoter

EF-1a

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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CMV-mCherry-3’UTR of β-actin-(F30-2xdBroccoli)6

Plasmid

#190162

Purpose

Expresses 24 Broccoli-tagged beta-actin mRNA in mammalian cells

Depositor

Samie Jaffrey

Article

Li et al Angew Chem Int Ed Engl. 2020 Mar 9;59(11

Insert

mcherry_(3'UTR, No Actin)_24Broccoli

Tags

24×Broccoli

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FLEX-RiboL1-jGCaMP8m

Plasmid

#192617

Purpose

AAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8m.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

FLEX-RiboL1-jGCaMP8m

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-EGFP-anti-rabbit-IgG(Fc)-nanobody

Plasmid

#138133

Purpose

Expresses GST-tagged EGFP-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

EGFP-anti-rabbit-IgG(Fc)-nanobody

Tags

GST

Expression

Bacterial

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-N-TEV-Twin-Strep

Plasmid

#202526

Purpose

Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cell

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Type

Empty backbone

Tags

TEV cleavable site and Twin-strep-tag

Expression

Mammalian

Promoter

CAG

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry_P2AT2A_GFP-nb-KS-FtoA

Plasmid

#238234

Purpose

For overexpression of mCherry_P2AT2A_GFP-nb-KS-FtoA

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry_P2AT2A_GFP-nb-KS-FtoA

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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tdTomato-Mito-7

Plasmid

#58115

Purpose

Localization: Mitochondria, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Article

Ai et al Nat Methods. 2008 May;5(5):401-3. doi: 1

Insert

Mito (COX8A Human)

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)/Luc2=tdT

Plasmid

#32904

Purpose

Expresses a fusion of firefly luciferase and tandem Tomato red fluorescent protein in mammalian cells.

Depositor

Christopher Contag

Article

Patel et al Cancer Res. 2010 Dec 15;70(24):10141-9.

Inserts

Firefly Luciferase
tandem Tomato Red Fluorescent Protein

Tags

tdTomato red fluorescent protein

Expression

Mammalian

Promoter

CMV-IE and n/a

Available Since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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