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We narrowed to 6,905 results for: crispr cas9 plasmids

Showing: 6421 - 6440 of 6905 results

  1. pGG438

    Plasmid
    #165486
    Purpose
    Vector for expression of the SpCas9 VRKG variant in human cells: CMV-T7-humanVRKG(SpCas9, D1135V/S1136R/D1332K/R1333G)-1xNLS(SV40)-3xFLAG
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    Mammalian codon-optimized Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G)-1xNLS(SV40)-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    D1135V, S1136R, D1332K, and R1333G mutations in S…
    Promoter
    CMV and T7
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165486-plasmid-map-sequence-id-330963

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  3. pRS159

    Plasmid
    #122378
    Purpose
    CRISPRi plasmid for use in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)
    Depositor
    Rebecca Shapiro
    Article
    Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    122378-plasmid-map-sequence-id-241243

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  7. pET-28b-T7-henAsCas12a(E174R/S542R/K548R)-NLS(nucleoplasmin)-6xHis (AAS1885)

    Plasmid
    #114072
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized enAsCas12a(E174R/S542R/K548R) with C-terminal NLS and His-tag
    Depositor
    Benjamin Kleinstiver, Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a (E174R/S542R/K548R) with C-terminal NLS and His-tag
    Use
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R, S542R, and K548R
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114072-plasmid-map-sequence-id-220522

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  8. pET-28b-T7-hAsCas12a-enRR(E174R/S542R/K607R)-NLS(nucleoplasmin)-6xHis (AAS1902)

    Plasmid
    #114077
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-enRR(E174R/S542R/K607R) with C-terminal NLS and His-tag
    Depositor
    Benjamin Kleinstiver, Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-enRR (E174R/S542R/K607R) with C-terminal NLS and His-tag
    Use
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R/S542R/K607R
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114077-plasmid-map-sequence-id-220525

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  9. pET-28b-T7-hAsCas12a(E174R/S542R)-NLS(nucleoplasmin)-6xHis (AAS1880)

    Plasmid
    #114071
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a(E174R/S542R) with C-terminal NLS and His-tag
    Depositor
    Benjamin Kleinstiver, Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a (E174R/S542R) with C-terminal NLS and His-tag
    Use
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R and S542R
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114071-plasmid-map-sequence-id-220509

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  10. pET-28b-T7-hAsCas12a-RR(S542R/K607R)-NLS(nucleoplasmin)-6xHis (AAS1916)

    Plasmid
    #114076
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-RR(S542R/K607R) with C-terminal NLS and His-tag
    Depositor
    Benjamin Kleinstiver, Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-RR (S542R/K607R) with C-terminal NLS and His-tag
    Use
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    S542R and K607R
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114076-plasmid-map-sequence-id-220531

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  11. pET-28b-T7-hAsCas12a-RVR(S542R/K548V/N552R)-NLS(nucleoplasmin)-6xHis (AAS1897)

    Plasmid
    #114074
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-RVR(S542R/K548V/N552R) with C-terminal NLS and His-tag
    Depositor
    Benjamin Kleinstiver, Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-RVR (S542R/K548V/N552R) with C-terminal NLS and His-tag
    Use
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    S542R, K548V and N552R
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114074-plasmid-map-sequence-id-220510

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  19. pJEP324-pAAV-FullH1TO-SaCa9sgRNAi(emx1sg1)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA

    Plasmid
    #113701
    Purpose
    Dox-inducible H1 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1. Followed by an EFS driven GFP-KASH in a separate reading frame.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 gRNA Cassete
    GFP
    Use
    AAV and CRISPR
    Tags
    KASH
    Expression
    Mutation
    Promoter
    Available since
    Feb. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    113701-plasmid-map-sequence-id-232036

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  20. pJEP325-pAAV-FullH1TO-SaCa9sgRNAi(CREB)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA

    Plasmid
    #113702
    Purpose
    Dox-inducible H1 driven SaCas9 gRNA expression cassette with a gRNA targeting CREB. Followed by an EFS driven GFP-KASH in a separate reading frame.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 gRNA Cassete
    GFP
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    KASH
    Expression
    Mutation
    Promoter
    Available since
    Feb. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    113702-plasmid-map-sequence-id-229517

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Showing: 6421 - 6440 of 6905 results