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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 9,642 results for: Ada;

Showing: 6461 - 6480 of 9642 results

  2. pNOC_dCas9_sgRNA NC

    Plasmid
    #176259
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI and
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Expression
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Promoter
    Available since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    176259-plasmid-map-sequence-id-346581

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  3. pAVA-LGF2(fs)-Gal11p(fs)

    Plasmid
    #127483
    Purpose
    Negative control for the AVA-seq system. LGF2 (with one nucleotide insertion) (lambda CI associated) and Gal11p (with one nucleotide insertion) (RNAp associated) interaction
    Depositor
    Joel Malek
    Article
    Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1
    Insert
    LGF2 (frame shift)-Gal11p (frame shift) (GAL11 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    One nucleotide inserted between lambda CI and LGF…
    Promoter
    Available since
    Nov. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    127483-plasmid-map-sequence-id-262924

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  5. pAAV-UBC-S3-RAB_EKARev

    Plasmid
    #160727
    Purpose
    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S3 protein scaffold.
    Depositor
    Edward Boyden
    Article
    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31
    Insert
    S3-RAB_EKARev
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Human ubiquitin C (UBC) promoter
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160727-plasmid-map-sequence-id-315764

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  7. pKK-EGFP-TEV

    Plasmid
    #105776
    Purpose
    Expression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 8885998, 8805248). EGFP is not truly monomeric (PMID: 11988576, 22869113)
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    EGFP-TEV
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105776-plasmid-map-sequence-id-203365

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  8. pAAV-EFS-DIO-GRAB_CRF1.0

    Plasmid
    #208662
    Purpose
    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in a cre-dependent manner
    Depositor
    Yulong Li
    Article
    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.
    Insert
    GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    EFS
    Available since
    Dec. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    208662-plasmid-map-sequence-id-417680

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  15. pKK-mTagBFP-TEV

    Plasmid
    #105772
    Purpose
    Expression of your protein of interest in fusion with blue fluorescent protein at the N-terminus (cleavable by TEV) (PMID: 18940671, 25378625). Useful for multi-channel live cell imaging.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    mTagBFP-TEV
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105772-plasmid-map-sequence-id-203361

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Showing: 6461 - 6480 of 9642 results