We narrowed to 10,454 results for: ESP

1. pMH-SFB-USP7

   Plasmid

   #99393

   Purpose

   Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged USP7

   Depositor

   Michael Huen

   Article

   An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

   Insert

   USP7 (USP7 Human)
   

   Tags

   SFB (S protein-FLAG-Streptavidin binding peptide)

   Expression

   Mammalian

   Available Since

   Aug. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST-DHFR F[1,2]-N (TRP1)

   Plasmid

   #177797

   Purpose

   Gateway destination vector to insert genes of interest having a N-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.

   Depositor

   Christian Landry

   Article

   Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

   Type

   Empty backbone

   Tags

   DHFR F[1,2]

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-MTS-CA-c-Src-FLAG

   Plasmid

   #44654

   Depositor

   Yoshimi Homma

   Article

   Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

   Insert

   v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
   

   Tags

   FLAG tag and mitochondria-targeting sequence (MTS…

   Expression

   Mammalian

   Mutation

   changed Tyr 530 to Phe

   Promoter

   CMV promoter

   Available Since

   May 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pαH-S-RRAR/D614G

   Plasmid

   #164570

   Purpose

   SARS-CoV-2 Spike protein with D614G mutation and Furin site restored (S-RRAR-D614G variant)

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

   Insert

   Spike (S-RRAR-D614G variant)
   

   Tags

   2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

   Expression

   Mammalian

   Mutation

   D614G

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBABE-puro p21

   Plasmid

   #78783

   Purpose

   Retroviral expression of p21

   Depositor

   Jaewhan Song

   Article

   Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

   Insert

   p21 (CDKN1A Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   5'LTR

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV_ER-RE_MLPmin_BC1065-luc2

   Plasmid

   #227111

   Purpose

   Estrogen receptor response element for luciferase and barcode assays; barcode BC1065

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   12x clustered ER response element linked to MLP minimal promoter driving barcode 1065 and a luciferase reporter gene
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. SARS-COV2-NSP1-HA-BASU

   Plasmid

   #154071

   Purpose

   Express SARS-CoV-2 NSP1 gene along with HA and BASU

   Depositor

   Paul Khavari

   Article

   Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

   Insert

   Nsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcsII-pb4-flag

   Plasmid

   #86768

   Purpose

   C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

   Depositor

   Do-Hyung Kim

   Article

   Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

   Insert

   proteasome beta subunit 4 (PSMB4 Human)
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMXs-WT-ATPIF1

   Plasmid

   #85403

   Purpose

   Retroviral vector expressing wild type human ATPIF1

   Depositor

   David Sabatini

   Article

   Chen et al Cell Rep. 2014 Apr 10;7(1):27-34. doi: 1

   Insert

   ATPIF1 (ATP5IF1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Wildtype

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pCAG-mIRFP670nuc-TEV-Xph20-ETWV

    Plasmid

    #133044

    Purpose

    Expresses Xph20-ETWV in mammalian cells with nuclear miRFP670 as reporter

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

    Insert

    Xph20-ETWV
    

    Tags

    NLS, TEV protease + TEV cleavage site, and miRFP6…

    Expression

    Mammalian

    Mutation

    S63K

    Promoter

    CAG

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLJM1-PHD2

    Plasmid

    #223552

    Purpose

    Lentivirus transfer plasmid for expression of full length human PHD2

    Depositor

    Somshuvra Mukhopadhyay

    Article

    Gurol et al Proc Natl Acad Sci U S A. 2024 Jun 25;12

    Insert

    PHD2 (EGLN1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-MTS-KD-c-Src-FLAG

    Plasmid

    #44653

    Depositor

    Yoshimi Homma

    Article

    Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

    Insert

    v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
    

    Tags

    FLAG tag and mitochondria-targeting sequence (MTS…

    Expression

    Mammalian

    Mutation

    changed lysine 298 to methionine

    Promoter

    CMV promoter

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-GG-OCT4-1-5-PGK-Puro

    Plasmid

    #102893

    Purpose

    PiggyBac transposon system construct with 5 concatenated U6 promoter driven transcriptional cassettes for the activation of OCT4. Contains PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    OCT4-gRNAs-1-5-PGK-puro (POU5F1 )
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFastBac1_GI.1_N116C-G193C

    Plasmid

    #162580

    Purpose

    Expresses norovirus GI.1 VP1 protein with mutations N116C-G193C in Sf9 insect cells

    Depositor

    Peter Kwong

    Article

    Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

    Insert

    VP1 protein from human norovirus GI.1
    

    Expression

    Insect

    Mutation

    Asparagine 116 was mutated to Cysteine and Glycin…

    Promoter

    polyhedrin

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. p426_Cas9_gRNA-ARS416d

    Plasmid

    #87386

    Purpose

    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS416d sequence TAGTGCACTTACCCCACGTT in yeast chromosome 4.

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS416d
    

    Use

    CRISPR

    Promoter

    ADH1, pTyrosine

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. mEos3.2trCD45

    Plasmid

    #203753

    Purpose

    Encodes the transmembrane domain from CD45 with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    trCD45 (PTPRC Human)
    

    Tags

    mEos3.2

    Expression

    Mammalian

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAN19-3xFLAG-ccvTIR1-NOSt

    Plasmid

    #108546

    Purpose

    Cloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 with the F79G mutation [ccvTIR1] and NOS terminator

    Depositor

    Keiko Torii

    Article

    Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

    Insert

    TRANSPORT INHIBITOR RESPONSE 1 [F79G] fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)
    

    Use

    Cloning vector

    Tags

    3xFLAG

    Mutation

    Changed F79 to G

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCold I-Hero11

    Plasmid

    #187928

    Purpose

    Bacterial expression of heat-resistant obscure (Hero) protein Hero11

    Depositor

    Yukihide Tomari

    Article

    Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

    Insert

    Hero11 (C19orf53 Human)
    

    Tags

    Flag-6xHis

    Expression

    Bacterial

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. px458_2A_GFP_sgRNA_EIF2AK2

    Plasmid

    #106108

    Purpose

    CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting EIF2AK2

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    gRNA targeting EIF2AK2 (EIF2AK2 Human)
    

    Use

    CRISPR

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. CaMKIV-DCTK75E

    Plasmid

    #126423

    Purpose

    A kinase-dead mutant of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)

    Depositor

    Douglas Black

    Article

    Xie et al Nature. 2001 Apr 19;410(6831):936-9. doi

    Insert

    calcium/calmodulin-dependent protein kinase IV kinase-dead mutant (CAMK4 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed lysine 75 to glutamic acid

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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