We narrowed to 8,596 results for: Set

1. pMVV098

   Plasmid

   #215304

   Purpose

   Expresses sfGFP under induction with the DAPG Marionette Cassette (PhIFAM + PPhIF-sfGFP) with a BBR1 backbone.

   Depositor

   Brian Pfleger

   Article

   Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Tags

   None

   Expression

   Bacterial

   Promoter

   PPhIF

   Available Since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAEF5

   Plasmid

   #136305

   Purpose

   Plasmid encoding the Cas9 gene and a gRNA expression cassette allowing to clone a single or multiple gRNAs for DSBs induction in yeast. Constructed by Aubin Fleiss

   Depositor

   Gilles Fischer

   Article

   Agier et al Methods Mol Biol. 2021;2196:181-198. doi

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Feb. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTAJAK-71 (pESC-NatMXsyn-USER)

   Plasmid

   #78232

   Purpose

   Empty vector, for the insertion of gRNA expression cassettes into the vector.

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR2B-dCas9-KRAB

   Plasmid

   #115547

   Purpose

   Entry cassette for dCas9-KRAB

   Depositor

   Rene Maehr

   Article

   Genga et al Cell Rep. 2019 Apr 16;27(3):708-718.e10.

   Insert

   dCas9-KRAB
   

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Available Since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS AluYa5rescueA70D

   Plasmid

   #78657

   Purpose

   Generates Alu inserts suitable for rescue

   Depositor

   Astrid Roy-Engel

   Article

   Wagstaff et al PLoS Genet. 2012;8(8):e1002842. doi: 10.

   Insert

   Ya5rescue cassette
   

   Available Since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. MTK8b_004

   Plasmid

   #123892

   Purpose

   Encodes the 3rd gen. 5' HIV LTR cassette as a type 8b part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   5' HIV LTR (3rd gen.)
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAC1750

   Plasmid

   #127984

   Purpose

   AMA1 plasmid with Aspergillus optimized Cpf1, ble selection marker, and USER cassette (PacI/Nt.BbvCI)

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

   Insert

   Cpf1
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   codon optimized for Aspergillus nidulans

   Promoter

   Aspergillus nidulans tef1 promoter

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV GG hUbV-EGFP

   Plasmid

   #216161

   Purpose

   Contains a Golden-Gate cloning cassette to express up to four gRNA and EGFP

   Depositor

   Makiko Iwafuchi

   Article

   Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAL119

   Plasmid

   #21909

   Depositor

   Maria Castro

   Article

   Ali et al Mol Ther. 2004 Dec . 10(6):1071-84.

   Insert

   hCMV promoter expression cassette
   

   Use

   Adenoviral

   Available Since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pgRNA-backbone

    Plasmid

    #172517

    Purpose

    Yeast plasmid encoding a 2-kb gRNA-placeholder flanked by BsmBI/Esp3I restriction sites to facilitate gRNA insertion

    Depositor

    Leonid Kruglyak

    Article

    Schubert et al Elife. 2022 Nov 3;11. pii: 79525. doi: 1

    Insert

    gRNA cassette
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUF-AsCpf1-pre-gRNA

    Plasmid

    #137849

    Purpose

    AsCpf1 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

    Depositor

    Josh Beck

    Article

    Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

    Insert

    AsCpf1 and pre-gRNA cassette
    

    Use

    CRISPR

    Available Since

    April 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. FRP1641_PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1

    Plasmid

    #58441

    Purpose

    Cassette to replace yeast promoters with an inducible promoter containing 3 lexA boxes

    Depositor

    Joerg Stelling

    Article

    Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

    Insert

    PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1
    

    Expression

    Yeast

    Promoter

    PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL4-Gateway-SCP1

    Plasmid

    #71510

    Purpose

    humanSTARR-seq validation vector with Gateway cassette upstream of the firefly luciferase gene and the SCP1 core promoter

    Depositor

    Alexander Stark

    Article

    Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

    Type

    Empty backbone

    Use

    Humanstarr-seq validation vector

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    Jan. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

14. MTK7_014

    Plasmid

    #123881

    Purpose

    Encodes the extended 3rd gen. 3' HIV LTR cassette as a type 7 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    3' HIV LTR (3
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSCL.028

    Plasmid

    #184965

    Purpose

    Test effect of extending -Eco1 a1/a2 in yeast

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: RT and ncRNA(extended), a1/a2 length: 27
    

    Expression

    Yeast

    Mutation

    human codon optimized RT, a1/a2 length extended t…

    Promoter

    Gal7

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDYSCKO-ADE1

    Plasmid

    #120264

    Purpose

    pDYSCKO canavanine co-selection vector with a guide targeting the ADE1 locus

    Depositor

    Christian Landry

    Article

    Despres et al G3 (Bethesda). 2018 Oct 3;8(10):3163-317

    Insert

    pDYSCKO cassette-ADE1 targeting guide
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    SNR52

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUF-LbCpf1-pre-gRNA

    Plasmid

    #137848

    Purpose

    LbCpf1 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

    Depositor

    Josh Beck

    Article

    Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

    Insert

    LbCpf1-NLS-FLAG and gRNA cassette
    

    Use

    CRISPR

    Available Since

    April 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-EEA-g1-PGK-Puro

    Plasmid

    #102908

    Purpose

    PiggyBac transposon system construct for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    EEA-guide1-PGK-Puro
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. MXS_PGK::tTA2-bGHpA

    Plasmid

    #62448

    Purpose

    MXS_chaining vector with PGK::tTA2-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    cassette containing the tetracycline transactivator with PGK promoter
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. MXS_PGK::rtTA3-bGHpA

    Plasmid

    #62446

    Purpose

    MXS_chaining vector with PGK::rtTA3-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    cassette containing the reverse tetracycline transactivator with PGK promoter
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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