We narrowed to 11,757 results for: pcdna

1. IFI16-(515-710)-HinB (pcDNA3-FLAG)

   Plasmid

   #35063

   Depositor

   Cheryl Arrowsmith

   Article

   Liao et al Structure. 2011 Mar 9;19(3):418-29.

   Insert

   IFI16-(515-710)-HinB (IFI16 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains HinB domain: aa 515-710

   Promoter

   CMV

   Available since

   April 4, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3.1 3xFLAG TPP1 88-544 delta166-172

   Plasmid

   #53551

   Purpose

   mammalian expression of TPP1 full length with 166-172 replaced with "GSSG"

   Depositor

   Kathleen Collins

   Article

   Sexton et al J Biol Chem. 2012 Oct 5;287(41):34455-64

   Insert

   TPP1 (ACD Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   contains aa 88-544, with aa 166-172 replaced wit…

   Promoter

   CMV

   Available since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA5-EGFP-NLS-T2A-mCherry-PTS1

   Plasmid

   #87827

   Purpose

   Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using T2A. NLS and PTS1 can be replaced by proteins of interest.

   Depositor

   Andrea Musacchio

   Article

   Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

   Inserts

   NLS
   PTS1
   

   Use

   Tags

   EGFP and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter, tetracycline operator

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1-TagBFP-P2A-Pre-SPHERE-SF-iGluSnFR

   Plasmid

   #187089

   Purpose

   Glutamate sensor for localization to membrane contatct

   Depositor

   Kotaro Oka

   Article

   Shindo et al bioRxiv 2022

   Insert

   Pre-SPHERE-SF-iGluSnFR
   

   Use

   Tags

   TagBFP-P2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. YFP(159-238)-gamma-2 in pcDNAI/Amp

   Plasmid

   #54472

   Purpose

   A carboxyl-terminal YFP fragment was fused to Ggamma-2. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.

   Depositor

   Catherine Berlot

   Article

   Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

   Insert

   YFP(159-238)-gamma -2 (GNG2 Human, Aequorea victoria)
   

   Use

   Tags

   YFP(159-238) was fused to the amino terminus of G…

   Expression

   Mammalian

   Mutation

   Ggamma-2 was amplified by PCR, which added a BamH…

   Promoter

   CMV

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3_SP-GLP1R-V2R-NTEV-TCS-GV-2xHA

   Plasmid

   #194381

   Purpose

   Split TEV assays

   Depositor

   Michael Wehr

   Article

   Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

   Insert

   SP-GLP1R-V2R-NTEV-TCS-GV-2xHA (GLP1R Synthetic, Human)
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.2 mir-1-1 reporter hsa-mir-133a-1

   Plasmid

   #46676

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   hsa-mir-133a-1 (MIR133A1 Human)
   

   Use

   RNAi

   Tags

   V5 and hsa-mir-1-1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. 5'-3xFLAG Chtop-exon minimal CMV pCDNA5

   Plasmid

   #212080

   Purpose

   LR vector for integration of Chtop-exon into N2a FRT rtTA3 expression cells

   Depositor

   Benjamin Blencowe

   Article

   Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

   Insert

   Chtop-exon (Chtop Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-CAG-LMO1 (GLuc-ChR2-EYFP)

   Plasmid

   #72888

   Purpose

   fusion protein of Gaussia luciferase, Channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Berglund et al PLoS One. 2013;8(3):e59759. doi: 10.1371

   Insert

   Gaussia luciferase, Channelrhodopsin-2, EYFP
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.2 mir-1-1 reporter hsa-mir-122

    Plasmid

    #46673

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    hsa-mir-122 (MIR122 Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA 3.1(-) rat C/EBP beta (LAP)

    Plasmid

    #12558

    Depositor

    Peter Johnson

    Insert

    CCAAT/Enhancer-binding Protein beta (Cebpb Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3_SARS-CoV-Spike[SARS-CoV-2 RBD]

    Plasmid

    #190086

    Purpose

    express chimeric SARS-CoV spike with the SARS-CoV-2 receptor binding domain

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

    Insert

    SARS-CoV S (S Synthetic)
    

    Use

    Tags

    Rhodopsin C9

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1-TagBFP-P2A-Pre-SPHERE-iGABASnFR

    Plasmid

    #187091

    Purpose

    GABA sensor for localization to membrane contatct

    Depositor

    Kotaro Oka

    Article

    Shindo et al bioRxiv 2022

    Insert

    Pre-SPHERE-iGABASnFR
    

    Use

    Tags

    TagBFP-P2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-iRFP-P2A-Post-SPHERE-iGABASnFR

    Plasmid

    #187092

    Purpose

    GABA sensor for localization to membrane contatct

    Depositor

    Kotaro Oka

    Article

    Shindo et al bioRxiv 2022

    Insert

    Post-SPHERE-iGABASnFR
    

    Use

    Tags

    iRFP670-P2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(+)/AUG-nLuc-3XFLAG-CL1/PEST

    Plasmid

    #127315

    Purpose

    Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG and CL1/PEST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-Dakap-Venus-cpVenus-FLARE-AKAR

    Plasmid

    #123338

    Purpose

    Mitochondria-targeted yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    DAKAP-Venus-cpVenus-FLARE-AKAR
    

    Use

    Tags

    N-terminal 30 amino acids from DAKAP1, Venus, and…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-iRFP-P2A-Post-SPHERE-SF-iGluSnFR

    Plasmid

    #187090

    Purpose

    Glutamate sensor for localization to membrane contatct

    Depositor

    Kotaro Oka

    Article

    Shindo et al bioRxiv 2022

    Insert

    Post-SPHERE-SF-iGluSnFR
    

    Use

    Tags

    iRFP670-P2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Nrf1-Myc pcDNA3.1(+)-C-Myc (NAWLVH106-AAAAAA)

    Plasmid

    #169126

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hNrf1 (NFE2L1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NAWLVH106-AAAAAA

    Promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5/FRT/TO_H2B-HaloTag10-T2A-EGFP

    Plasmid

    #175521

    Purpose

    Nuclear expression of HaloTag10 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag10 = HaloTag7-Q165W

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3 Flag FKHR H215R AAA mutant

    Plasmid

    #13510

    Depositor

    Kunliang Guan

    Article

    Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

    Insert

    FKHR H215R AAA (FOXO1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    H215R T24A S256A S319A

    Promoter

    Available since

    Dec. 11, 2006

    Availability

    Academic Institutions and Nonprofits only