We narrowed to 7,534 results for: ski

1. pET28a_DmKHC[1-421]-SNAP-6xHis

   Plasmid

   #196975

   Purpose

   Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal SNAP tag and C-terminal 6xHis tag

   Depositor

   Lukas Kapitein

   Article

   Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015

   Insert

   Kinesin Heavy Chain (Khc Fly)
   

   Tags

   6xHis and SNAP

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-pegRNA-HEK3-CTTins-px330-scaffold

   Plasmid

   #180017

   Purpose

   Transiently expressing a pegRNA to introduce HEK3 CTT insertion in human cells. It has a sgRNA scaffold from px330.

   Depositor

   Dirk Hockemeyer

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   Prime editing pegRNA for HEK3-CTTins, with px330 scaffold (EPHA8 Synthetic)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Human U6

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223_RALA_WT_V5

   Plasmid

   #82988

   Purpose

   Gateway Donor vector containing RALA, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, Matthew Meyerson, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   RALA (RALA Human)
   

   Use

   Gateway entry vector

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-TetOne-FFSS-Cyclin D1 (T286A)

   Plasmid

   #215147

   Purpose

   Expresses FFSS-Cyclin D1 (T286A) in mammalian cells from an inducible lentiviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Lentiviral

   Tags

   FFSS (FLAG-FLAG-STREP-STREP)

   Expression

   Mammalian

   Mutation

   With T286A mutation.

   Promoter

   TRE3GS

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.CAG-FLEX.iAChSnFR-Venus-NULL

   Plasmid

   #137960

   Purpose

   Expresses FLEXed iAChSnFR (non-binding yellow version) under CAG promoter

   Depositor

   Loren Looger

   Article

   Borden et al bioRxiv 2020

   Insert

   iAChSnFR (non-binding yellow variant)
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Mutation

   Y140A binding site mutation

   Promoter

   CAG

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXs-Sox2-17

   Plasmid

   #193353

   Purpose

   Retroviral vector expressing mouse Sox2-17 chimeric transcription factor (super-Sox)

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   Sox2-17 (Sox2 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Sox2-17 is engineered highly cooperative chimeric…

   Promoter

   5'LTR

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223_NFIL3_WT_V5

   Plasmid

   #82985

   Purpose

   Gateway Donor vector containing NFIL3, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, Matthew Meyerson, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   NFIL3 (NFIL3 Human)
   

   Use

   Gateway entry vector

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL3-basic-FTO promoter_WT

   Plasmid

   #108922

   Purpose

   The core promoter region of FTO was inserted into pGL3 basic vector

   Depositor

   Jianjun Chen

   Article

   Su et al Cell. 2018 Jan 11;172(1-2):90-105.e23. d

   Insert

   FTO (FTO Human)
   

   Tags

   NA

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223_CCL2_WT_V5

   Plasmid

   #82935

   Purpose

   Gateway Donor vector containing CCL2, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, Matthew Meyerson, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   CCL2 (CCL2 Human)
   

   Use

   Gateway entry vector

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-PGK-RAP1A-WT-neo

    Plasmid

    #156173

    Purpose

    Lentiviral vector for expression of RAP1A-WT, with neomycin section

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP1A (RAP1A Human)
    

    Use

    Lentiviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    wild type form

    Promoter

    human PGK

    Available Since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-T7-ABE8e-SpCas9-HiFi-P2A-EGFP (LM446)

    Plasmid

    #197506

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HiFi(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpCas9-HiFi-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-HiFi(D10A/R69…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6-pegRNA-LRRK2-G2019S-3a

    Plasmid

    #180432

    Purpose

    Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

    Depositor

    Frank Soldner

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    ncRNA targeting LRRK2-G2019S (LRRK2 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    LRRK2-G2019S

    Promoter

    U6

    Available Since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL3-basic-FTO promoter_Mut

    Plasmid

    #108923

    Purpose

    The core promoter region of FTO with mutant CEBPA binding sites was inserted into pGL3 basic vector

    Depositor

    Jianjun Chen

    Article

    Su et al Cell. 2018 Jan 11;172(1-2):90-105.e23. d

    Insert

    FTO (FTO Human)
    

    Tags

    NA

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCherry-APTX

    Plasmid

    #215128

    Purpose

    Expresses mCherry-APTX in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    APTX (APTX Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQC hGAB.wt V5-His IRES G418

    Plasmid

    #110333

    Purpose

    Mammalian retroviral expression of glutaminase 2 isoform GAB (wild type) with V5 and 6xHis tags

    Depositor

    Sandra Martha Gomes Dias

    Article

    Dias et al Oncogene. 2020 Jan;39(3):690-702. doi: 1

    Insert

    GLS2 Glutaminase 2 (GLS2 Human)
    

    Use

    Retroviral

    Tags

    6xHis and V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-PGK-flag-RAP1B-V12-puro

    Plasmid

    #156168

    Purpose

    Lentiviral vector for expression of flag-RAP1B-V12, with puromycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP1B (RAP1B Bovine)
    

    Use

    Lentiviral

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    changed Glycine 12 to Valine

    Promoter

    human PGK

    Available Since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. p21-mCerulean-NeoR

    Plasmid

    #215080

    Purpose

    Expresses p21-mCerulean in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CDKN1A (CDKN1A Human)
    

    Tags

    mCerulean

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR223_EGFR_p.T790M

    Plasmid

    #82848

    Purpose

    Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    EGFR (EGFR Human)
    

    Use

    Gateway entry vector

    Mutation

    T790M

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-PGK-RAP1A-E63-neo

    Plasmid

    #156174

    Purpose

    Lentiviral vector for expression of RAP1A-E63, with neomycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP1A (RAP1A Human)
    

    Use

    Lentiviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    changed Glutamine 63 to Glutamic acid

    Promoter

    human PGK

    Available Since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR223_PIK3CA_p.E453Q

    Plasmid

    #82844

    Purpose

    Gateway Donor vector containing PIK3CA, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    NM_006218 (PIK3CA Human)
    

    Use

    Gateway entry vector

    Mutation

    E453Q

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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