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tetO-ESRRA

Plasmid

#170683

Purpose

doxycycline-inducible overexpression of ESRRA

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

ESRRA (ESRRA Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Esrrb

Plasmid

#71152

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Esrrb

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

Estrogen related receptor, beta (Esrrb Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

tetO

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pQCXIH BRGI-Flag

Plasmid

#19148

Depositor

Joan Massague

Article

Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

Insert

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only
CRHR2-DuET

Plasmid

#213216

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

CRHR2 (CRHR2 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMYC BRGI aa597-1407

Plasmid

#19146

Depositor

Joan Massague

Article

Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

Insert

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)

Tags

Myc

Expression

Mammalian

Mutation

human BRG1 aa 597-1407 (PCR product)

Available Since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only

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pNL3.1-Erg

Plasmid

#182706

Purpose

Express Erg enhancer in mammalian cells

Depositor

Robert Rowe

Article

Wang et al Cell Rep. 2022 Apr 5;39(1):110587. doi:

Insert

Enhancer of Erythroblast transformation-specific-Related Gene (Erg Mouse)

Use

Luciferase

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMYC BRGI aa1400-1648

Plasmid

#19145

Depositor

Joan Massague

Article

Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

Insert

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)

Tags

Myc

Expression

Mammalian

Mutation

human BRG1 aa 1400-1648 (PCR product)

Available Since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only
MSCV-shCasp2-IRES-GFP

Plasmid

#52061

Purpose

stable knockdown of Caspase-2 in mammalian cells

Depositor

Tyler Jacks, Trudy Oliver

Article

Oliver et al Mol Cell. 2011 Jul 8;43(1):57-71. doi: 1

Insert

Caspase-2 shRNA (CASP2 Human)

Use

Retroviral

Tags

IRES-EGFP

Expression

Mammalian

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
pBS L30 Ruby3-Rab7A

Plasmid

#135651

Purpose

Rab7A fused to the red fluorescent protein Ruby3 in N-ter under control of a weak L30 promoter.

Depositor

Raphael Gaudin

Article

Deffieu et al Sci Adv. 2021 Jan 8;7(2). pii: 7/2/eaba7

Insert

Rab7A (RAB7A Human)

Tags

Ruby3

Expression

Mammalian

Promoter

L30

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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BAM2 X050_pECIA14

Plasmid

#114904

Purpose

Prey vector BAM2 X050_pECIA14 should be used with bait vector BAM2 X050_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT3G49670 (BAM2 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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KHBD00054

Plasmid

#34216

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG2275 (Jra Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pEMS1707

Plasmid

#29295

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple235 (TRH Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Available Since

Nov. 4, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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AP2u2-CMV-S_protein

Plasmid

#226456

Purpose

Mammalian expression of S protein of SARS-CoV2 under CMV promoter, tagged with 6His and released extracellular environment after expression for protein purification

Depositor

Urartu Seker

Article

Ozcelik et al ACS Pharmacol Transl Sci. 2024 Mar 21;7(

Insert

Spike Protein

Tags

6xHis Tag, Foldon Domain, and Thrombin Cleavage S…

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-EF1aL-E690Kmut_ABCA3-GFP

Plasmid

#188549

Purpose

Lentiviral vector allowing for EF1aL-driven constitutive expression of E690K mutant human ABCA3:GFP fusion protein

Depositor

Darrell Kotton

Article

Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

Insert

ABCA3:GFP (ABCA3 Human)

Use

Lentiviral

Tags

GFP

Mutation

amino acid E690->K690 (ABCA3 gene)

Promoter

EF1aL

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-EF1aL-W308Rmut_ABCA3-GFP

Plasmid

#188550

Purpose

Lentiviral vector allowing for EF1aL-driven constitutive expression of W308R mutant human ABCA3:GFP fusion protein

Depositor

Darrell Kotton

Article

Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

Insert

ABCA3 (ABCA3 Human)

Use

Lentiviral

Tags

GFP

Mutation

amino acid W308->R308 (ABCA3 gene)

Promoter

EF1aL

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_FKBP-V5-PRRX1

Plasmid

#215024

Purpose

AAV vector for knocking in an N-terminal tag (FKBP12F36V and V5) for human PRRX1

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

paired related homeobox 1 (PRRX1 Human)

Use

AAV

Tags

FKBP-V5

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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GRPR-DuET

Plasmid

#213306

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

GRPR (GRPR Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pT22UbGfpnE4PCh(#301)

Plasmid

#184066

Purpose

cyclofen-inducible GFP nuclear relocation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Zhang et al Chembiochem. 2018 Jun 18;19(12):1232-123

Insert

eGFP-nls-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Ubi

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMIR-Report-SPRED1-3'UTR (mut - miR-21 site1)

Plasmid

#62580

Purpose

Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The upstream miR-21 (site 1) binding site was mutated.

Depositor

Michael Ruppert

Article

Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

Insert

SPRED1 (SPRED1 Human)

Use

Luciferase

Mutation

miR-21 binding site 1 mutant (TAAGCTA --> TAGA…

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only
pMIR-Report-SPRED1-3'UTR (mut - miR-21 site 2)

Plasmid

#62581

Purpose

Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The downstream miR-21 binding site (site 2) was mutated.

Depositor

Michael Ruppert

Article

Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

Insert

SPRED1 (SPRED1 Human)

Use

Luciferase

Mutation

miR-21 binding site 2 mutant (TAAGCTA --> TAGA…

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only