We narrowed to 8,358 results for: BLI

1. pBACgus4x/cdc37/LRRK2 1867-2186_C2024S/C2025S

   Plasmid

   #40366

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Baculovirus

   Tags

   HisTev

   Expression

   Mutation

   aa1867-2186, C2024S/C2025S

   Promoter

   polH

   Available since

   Oct. 1, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pTRE2_CMV_Mex3a_t2a_mCherry

   Plasmid

   #223244

   Purpose

   tetO responsive expression of Mex3a in mammalian cells with t2a cleaved mCherry

   Depositor

   Stavros Lomvardas

   Insert

   Mex3a (Mex3a Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   amino acids conserved, underlying DNA sequence re…

   Promoter

   CMV

   Available since

   May 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLEX_307-SARS-CoV-2-NSP3-V5

   Plasmid

   #214429

   Purpose

   Lentiviral-mediated stable expression of SARS-CoV-2 NSP3 in mammalian cells

   Depositor

   Mohan Babu

   Insert

   SARS-CoV-2 NSP3 (part of SARS-CoV-2 ORF1ab) (ORF1ab )
   

   Use

   Lentiviral

   Tags

   V5 tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLD-puro-Cc-IFIT5_WT-VA

   Plasmid

   #191221

   Purpose

   Lentiviral expression of human IFIT5_WT in mammalian cells

   Depositor

   Mohan Babu

   Insert

   interferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)
   

   Use

   Lentiviral

   Tags

   VA tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLD-puro-Cc-SARS-CoV-2_NSP3-VA

   Plasmid

   #191214

   Purpose

   Lentiviral expression of SARS-CoV-2 NSP3 in mammalian cells

   Depositor

   Mohan Babu

   Insert

   Non structural protein 3 (ORF1ab SARS CoV-2)
   

   Use

   Lentiviral

   Tags

   VA tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLD-puro-Cc-SARS-CoV-2_NSP16-VA

   Plasmid

   #191215

   Purpose

   Lentiviral expression of SARS-CoV-2 NSP16 in mammalian cells

   Depositor

   Mohan Babu

   Insert

   Non structural protein 16 (ORF1ab SARS CoV-2)
   

   Use

   Lentiviral

   Tags

   VA tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG426-GAL-SoxN-A22-EGFP

   Plasmid

   #181731

   Purpose

   Galactose inducible expression of SoxN-A22-EGFP

   Depositor

   Alejandro Chavez

   Insert

   SoxN-A22-EGFP (SoxN Fly)
   

   Use

   Tags

   3xFLAG-EGFP

   Expression

   Yeast

   Mutation

   extended repeat of 22 Ala residues replacing nati…

   Promoter

   Available since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti Puro HA-Ubiquitin-KO

   Plasmid

   #236292

   Purpose

   Lentiviral vector expressing HA-tagged ubiquitin KO mutant

   Depositor

   Melina Fan, Addgene Research Program

   Insert

   Ubiquitin KO (UBB Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Single ubiquitin coding sequence containing KO mu…

   Promoter

   CMV

   Available since

   April 15, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pLenti Puro HA-Ubiquitin-WT

   Plasmid

   #236289

   Purpose

   Lentiviral vector expressing HA-tagged wild type ubiquitin

   Depositor

   Melina Fan, Addgene Research Program

   Insert

   Ubiquitin (UBB Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Single ubiquitin coding sequence (full length UBB…

   Promoter

   CMV

   Available since

   April 10, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pCAGGS-FLAG-BubR1-D911A

    Plasmid

    #200815

    Purpose

    expresses BubR1-D911A in mammalian cells

    Depositor

    Hung-Chun Chang

    Insert

    BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Change Aspartic acid 911 to Alanine

    Promoter

    CAG

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. 1993_pCCLsin.PPT.hPGK.KaedeCETN1.PRE

    Plasmid

    #231804

    Purpose

    Label centrosomes with lentivirus by encoding a fusion protein consisting of the photo-convertible protein Kaede fused to Centrin 1 (CETN1), an integral component of centrosomes.

    Depositor

    Annette Oxenius

    Insert

    Kaede-CETN1 (Cetn1 Mouse)
    

    Use

    Lentiviral

    Tags

    Kaede

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. hSyn-eGFP-DDHD2

    Plasmid

    #232463

    Purpose

    Expresses GFP tagged DDHD2 lipase in neurons

    Depositor

    Timothy Ryan

    Insert

    DDHD2 (DDHD2 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCJ1022

    Plasmid

    #228762

    Purpose

    Plasmid expressing Cas9 and 2 gRNAs for mouse Pkd2. Use for disruption of mouse Pkd2 in cultured cells.

    Depositor

    Bradley Yoder

    Insert

    Pkd2 gRNAs (Pkd2 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDGB3_alpha1_CBS-FRT-OCS-BeYDVRepRepA-tNos (GB4646)

    Plasmid

    #225438

    Purpose

    Transcriptional unit for copper- and flipase-regulated BeYDV Rep/RepA. OCSt, flanked by FRT sites, is embedded in the 5'UTR of miniDFR.

    Depositor

    Diego Orzaez

    Insert

    CBS-FRT-OCS-BeYDVRepRepA-tNos
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDGB3_alpha2_BeYDV geminino 1.0 (GB4480)

    Plasmid

    #225436

    Purpose

    BeYDV LIR1:2nd intron+eGFP CDS+Ter35S+p35S+1st intron:LIR1 in alpha2. Deconstructed replicon that needs BeYDV Rep for circularization and, thus, replication and eGFP transcription.

    Depositor

    Diego Orzaez

    Insert

    BeYDV geminino 1.0
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUPD2_miniDFR-FRT-OCSterm-FRT-miniDFR (GB4642)

    Plasmid

    #225430

    Purpose

    OCS terminator flanked by two flippase recognition target (FRT) sites located at position 112 of the SlDFR minimal promoter

    Depositor

    Diego Orzaez

    Insert

    miniDFR-FRT-OCSterm-FRT-miniDFR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEC2796 (pSpy0C4)

    Plasmid

    #220280

    Purpose

    integrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the sagB locus via homologous recombination

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSN993

    Plasmid

    #219961

    Purpose

    Expresses CMT2A mutant version of human KIF1Bß in C. elegans

    Depositor

    Shinsuke Niwa

    Insert

    unc-104 promoter::human KIF1Bß(Q98L) (KIF1B Human, Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSM045

    Plasmid

    #217399

    Purpose

    KmR, pBTRcK broad host range vector with BTE1(C12-specific fatty acid thioesterase codon-optimised for C. necator) under Ptrc

    Depositor

    Brian Pfleger

    Article

    Mishra et al J Ind Microbiol Biotechnol. 2024 Feb 16:

    Insert

    BTE1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PTrc

    Available since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLPC-C-SF-TAP-DEST-TLK1- M566T

    Plasmid

    #218831

    Purpose

    Expresses C-terminal Strep-FLAG-tagged M566T human TLK1 variant 5 in a retrovirus vector.

    Depositor

    Travis Stracker

    Insert

    TLK1 M566T (TLK1 Human)
    

    Use

    Tags

    FLAG-STREP

    Expression

    Mammalian

    Mutation

    M566T

    Promoter

    CMV

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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