We narrowed to 8,404 results for: APP

1. LCV2_LacZ_sgRNA_2

   Plasmid

   #155093

   Purpose

   lentiviral plasmid expressing Cas9 and gRNA targeting LacZ

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   LacZ_sgRNA_2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. TVBB C-term-mNeongreen-Puro

   Plasmid

   #169228

   Purpose

   Targeting vector backbone to support a knock-in of Linker-mNeongreen-P2A-Puro at the C-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked mNeongreen-P2A-Puro
   

   Use

   Targeting vector backbone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. TVBB C-term-mTurquoise2-Hygro

   Plasmid

   #169225

   Purpose

   Targeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Hygro at the C-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked mTurquoise2-P2A-Hygro
   

   Use

   Targeting vector backbone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. anti-Notch1_E6-pBIOCAM5

   Plasmid

   #39344

   Depositor

   John McCafferty

   Article

   Falk et al Methods. 2012 Sep;58(1):69-78. doi: 10.1

   Insert

   scFv-Fc-fusion specific for Notch1 (clone B9) (Notch1, NOTCH1 Mouse, Human)
   

   Use

   Tags

   3xFLAG, 6xHis, and human Fc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKK-TEV-MBP

   Plasmid

   #105787

   Purpose

   Expression of your protein of interest in fusion with MBP at the C-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-MBP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pWZLblasti-HA-ERK2 GOF

   Plasmid

   #53174

   Purpose

   Expresses HA tagged human ERK2 R67SD321N from blasticidin resistance retroviral vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   ERK2 (MAPK1 Mustard Weed, Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   R67S/D321N

   Promoter

   Available since

   June 24, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pDONR223 SARS-CoV2 TEV-ORF9B

   Plasmid

   #154425

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   ORF9B
   

   Use

   Gateway-compatible entry vector, with insert of o…

   Tags

   Expression

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   July 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pAAV-EF1a-FDIO-ArchT-GFP

   Plasmid

   #124640

   Purpose

   AAV-mediated FLPo-dependent expression of ArchT-GFP under EF1a promoter

   Depositor

   David Dupret

   Article

   Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

   Insert

   ArchT
   

   Use

   AAV; Flp/frt

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223-ZNF706

   Plasmid

   #88037

   Purpose

   Donor Vector containing ZNF706 transcription factor, part of the Human TFome Collection

   Depositor

   Marc Vidal

   Article

   Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

   Insert

   ZNF706 (ZNF706 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Last nucleotide of stop codon removed to allow fo…

   Promoter

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDH-EF1a-extGluc-NGFR

    Plasmid

    #104832

    Purpose

    extGluc luciferase for invivo Imaging, NGFR for flourescent detection or separation

    Depositor

    Irmela Jeremias

    Article

    Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

    Inserts

    extGLuc
    NGFR
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLC-BFP-FADD

    Plasmid

    #75166

    Purpose

    LentiCRISPR-BFP with sgRNA targeting human FADD

    Depositor

    Beat Bornhauser

    Article

    McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

    Insert

    FADD sgRNA (FADD Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. EF1a_MYC deltaNLS_P2A_Hygro_Barcode

    Plasmid

    #120506

    Purpose

    Barcoded lentiviral vector to express c-MYC deltaNLS in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYC H84NLS (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deletion of nuclear localization signal sequence

    Promoter

    EF1a

    Available since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR223-ZNF692

    Plasmid

    #88701

    Purpose

    Donor Vector containing ZNF692 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF692 (ZNF692 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKK-mRuby3-TEV

    Plasmid

    #105780

    Purpose

    Expression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 26879144). mRuby3 is brighter than mCherry and has shifted ex and em spectra.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    mRuby3-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCEP4-myc-CXCR4

    Plasmid

    #98946

    Purpose

    Mammalian expression plasmid for N-terminal myc-tagged human CXCR4

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CXCR4 (CXCR4 Human)
    

    Use

    Tags

    Signal/leader sequence from Influenza A virus HA …

    Expression

    Mammalian

    Mutation

    Full-length, wildtype

    Promoter

    CMV

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. p2XStrep-ATIC

    Plasmid

    #125685

    Purpose

    expresses human ATIC protein with an N-terminal 2XStrep affinity tag

    Depositor

    Stephen Benkovic

    Article

    Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

    Insert

    ATIC (ATIC Human)
    

    Use

    Tags

    2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKK-TEV-BirA-FLAG

    Plasmid

    #105786

    Purpose

    Expression of your protein of interest in fusion with BirA-FLAG at the C-terminus (cleavable by TEV). Search for protein partners using the BioID (PMID: 15707685, 22412018).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-BirA-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. EF1a_MYC Thr58Ala_P2A_Hygro_Barcode

    Plasmid

    #120513

    Purpose

    Barcoded lentiviral vector to express MYC Thr58Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYC Thr58Ala (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Point mutation changing Threonine to Alanine at a…

    Promoter

    EF1a

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKK-TEV-EGFP

    Plasmid

    #105793

    Purpose

    Expression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 8885998, 8805248). EGFP is not truly monomeric (PMID: 11988576, 22869113)

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKK-TEV-mCherry

    Plasmid

    #105796

    Purpose

    Expression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 15558047). mCherry descends from the first truly monomeric red FP mRFP1.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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