We narrowed to 13,360 results for: Mpl;

1. pCu-GFP-Atg17(416) (I325D)

   Plasmid

   #121412

   Purpose

   Expressing GFP-Atg17

   Depositor

   Daniel Klionsky

   Article

   Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

   Insert

   CUP1p-GFP-ATG17 ORF-CYC1 terminator
   

   Expression

   Yeast

   Mutation

   Isoleucine 325 to Aspartic acid

   Promoter

   CUP1 promoter

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCu-GFP-Atg17(416) (F317D)

   Plasmid

   #121411

   Purpose

   Expressing GFP-Atg17

   Depositor

   Daniel Klionsky

   Article

   Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

   Insert

   CUP1p-GFP-ATG17 ORF-CYC1 terminator
   

   Expression

   Yeast

   Mutation

   Phenylalanine 317 to Aspartic acid

   Promoter

   CUP1 promoter

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCu-GFP-Atg17(416) (L105D)

   Plasmid

   #121410

   Purpose

   Expressing GFP-Atg17

   Depositor

   Daniel Klionsky

   Article

   Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

   Insert

   CUP1p-GFP-ATG17 ORF-CYC1 terminator
   

   Expression

   Yeast

   Mutation

   Leucine 105 to Aspartic acid

   Promoter

   CUP1 promoter

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCu-GFP-Atg17(416)

   Plasmid

   #121408

   Purpose

   Expressing GFP-Atg17

   Depositor

   Daniel Klionsky

   Article

   Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

   Insert

   CUP1p-GFP-ATG17 ORF-CYC1 terminator
   

   Expression

   Yeast

   Promoter

   CUP1 promoter

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGAD-Atg17(L313D I314K)

   Plasmid

   #121390

   Purpose

   For Yeast-two-hybrid analysis

   Depositor

   Daniel Klionsky

   Article

   Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

   Insert

   ATG17
   

   Expression

   Yeast

   Mutation

   Leucine 313 to Aspartic acid; Isoleucine 314 to L…

   Promoter

   ADH1 promoter

   Available Since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCu-Atg17(416) (L105D)

   Plasmid

   #121405

   Purpose

   Expressing Atg17

   Depositor

   Daniel Klionsky

   Article

   Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

   Insert

   CUP1p-ATG17 ORF-CYC1 terminator
   

   Expression

   Yeast

   Mutation

   Leucine 105 to Aspartic acid

   Promoter

   CUP1 promoter

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCu-Atg17(416) (F317D)

   Plasmid

   #121406

   Purpose

   Expressing Atg17

   Depositor

   Daniel Klionsky

   Article

   Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

   Insert

   CUP1p-ATG17 ORF-CYC1 terminator
   

   Expression

   Yeast

   Mutation

   Phenylalanine 317 to Aspartic acid

   Promoter

   CUP1 promoter

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCu-Atg17(416) (I325D)

   Plasmid

   #121407

   Purpose

   Expressing Atg17

   Depositor

   Daniel Klionsky

   Article

   Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

   Insert

   CUP1p-ATG17 ORF-CYC1 terminator
   

   Expression

   Yeast

   Mutation

   Isoleucine 325 to Aspartic acid

   Promoter

   CUP1 promoter

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBMN mCherry-NBR1(F562A/Y731A/ΔUBD)

   Plasmid

   #119692

   Purpose

   For mammalian expression of LIR double mutant NBR1 without its UBD

   Depositor

   Michael Lazarou

   Article

   Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

   Insert

   NBR1 (NBR1 Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   F562A/Y731A & UBD deletion (1-906 ), See depo…

   Available Since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBMN iRFP670-OPTN(F178A/D474N)

    Plasmid

    #119694

    Purpose

    For mammalian expression of iRFP-tagged LIR/UBD double mutant OPTN

    Depositor

    Michael Lazarou

    Article

    Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

    Insert

    OPTN (OPTN Human)
    

    Use

    Retroviral

    Tags

    iRFP670

    Expression

    Mammalian

    Mutation

    F178A/D474N

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pACEBAC1-CoREST-C

    Plasmid

    #109159

    Purpose

    Encodes a human C-terminal CoREST fragment with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system

    Depositor

    Monika Golas

    Article

    Inui et al Protein Sci. 2017 May;26(5):997-1011. do

    Insert

    human C-terminal CoREST, sequence-optimized for insect cells (RCOR1 Human)
    

    Tags

    3xFLAG

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Jan. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJL15

    Plasmid

    #117430

    Purpose

    Expression of ARL2

    Depositor

    Richard Kahn

    Article

    Clark et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

    Insert

    ARL2 (ARL2 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRPL28exon1-(Nhe/Mfe) SpHIS5 (pNTI619)

    Plasmid

    #115432

    Purpose

    Vector for expressing RPL28 with mutagenized exon2

    Depositor

    Nicholas Ingolia

    Article

    McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

    Inserts

    RPL28 (RPL28 Budding Yeast)
    HIS5
    

    Expression

    Yeast

    Mutation

    exon 2 deleted, with MfeI / NheI cloning site

    Promoter

    Ashbya gospii TEF1 and endogenous

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFL I3

    Plasmid

    #113514

    Purpose

    Expression of I3 in insect cells (Baculovirus system)

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    I3 (PPP1R11 Human)
    

    Tags

    6*His

    Expression

    Insect

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC19_4xT790_28bp

    Plasmid

    #114720

    Purpose

    4 x 28 bp repeats of the EGFR WT allele used as SiMREPS analyte standard

    Depositor

    Nils Walter

    Article

    Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

    Insert

    EGFR (EGFR Human)
    

    Expression

    Bacterial

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC19_T790_28bp

    Plasmid

    #114722

    Purpose

    28 bp fragment of the WT EGFR allele used as SiMREPS analyte standard

    Depositor

    Nils Walter

    Article

    Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

    Insert

    EGFR (EGFR Human)
    

    Expression

    Bacterial

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCIG1_UL136_19kDa_EE

    Plasmid

    #74951

    Purpose

    Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 19kda isoform of UL136. Also expresses EGFP.

    Depositor

    Felicia Goodrum

    Article

    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

    Insert

    UL136 (UL136 Human Cytomegalovirus strain TB40/E)
    

    Use

    Lentiviral

    Tags

    Glu-Glu tag (EYMPME)

    Expression

    Mammalian

    Mutation

    delta 1-127

    Promoter

    CMV, lentiviral LTR

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCIG1_UL136_23kDa_Myc

    Plasmid

    #74949

    Purpose

    Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 23kda isoform of UL136. Also expresses EGFP.

    Depositor

    Felicia Goodrum

    Article

    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

    Insert

    UL136 (UL136 Human Cytomegalovirus strain TB40/E)
    

    Use

    Lentiviral

    Tags

    HA Tag (YPYDVPDYA)

    Expression

    Mammalian

    Mutation

    delta 1-99 M128A

    Promoter

    CMV, Lentiviral LTR

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA3-biotin-myc- mCE

    Plasmid

    #82475

    Purpose

    Expresses mRNA capping enzyme containing N terminal biotin and myc tag

    Depositor

    Daniel Schoenberg

    Article

    Mukherjee et al PLoS Biol. 2014 Aug 19;12(8):e1001933. d

    Insert

    Biotin (Rngtt Mouse)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a_6HT-TBP6.7(WT)

    Plasmid

    #112720

    Purpose

    Wild-type TBP6.7 was one of the first evolved TAR-binding proteins to bind with exceptional affinity. It was this protein that was used to obtain co-crystals with WT TAR RNA.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(WT)
    

    Tags

    6His-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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