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Parvalbumin scFv [L114/3]

Plasmid

#182072

Purpose

Mammalian Expression of Parvalbumin scFv. Derived from hybridoma L114/3.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Parvalbumin (Rattus norvegicus) (Pvalb Mouse)

Tags

HA, His

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Fon/Von GCaMP 6m

Plasmid

#137165

Purpose

Intersectional viral expression of GCaMP6M in cells expressing Cre AND Flp AND Vcre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Insert

3x-GCaMP6M

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

Removed RSET tag

Promoter

Ef1a

Available Since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-MOG [L94/54]

Plasmid

#190296

Purpose

Mammalian Expression Plasmid of anti-MOG (Rat). Derived from hybridoma L94/54.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-MOG (Rattus norvegicus) recombinant Mouse monoclonal antibody (Mog Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Fon-bREACHes-EYFP

Plasmid

#137145

Purpose

Intersectional viral expression of bREACHes-EYFP in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Insert

Con/Fon-bREACHes-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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Pan-Neurofascin scFv [L11A/41]

Plasmid

#206722

Purpose

Mammalian Expression of Pan-Neurofascin scFV. Derived from hybridoma L11A/41 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Pan-Neurofascin (Rattus norvegicus) recombinant scFV (Nfasc Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-OptoFGFR1-Y766F

Plasmid

#59777

Purpose

Expresses optoFGFR1 with mutation in PLC-gamma binding phosphotyrosine site of FGFR1

Depositor

Won Do Heo

Article

Kim et al Chem Biol. 2014 Jul 17;21(7):903-12. doi

Insert

OptoFGFR1-Y766F (FGFR1 Human, Mustard Weed)

Tags

mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-CDreV

Plasmid

#140133

Purpose

can be used to generate AAV virus that will express fusion protein of split Dre (C-terminal) and fungal photoreceptor Vivid (VVD) monomer

Depositor

Hongkui Zeng

Article

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Insert

CDreV

Use

AAV

Promoter

EF1a

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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RRL.sin.cPPT.SFFV/Ace2.WPRE (MT136)

Plasmid

#145842

Purpose

Lentiviral vector genome to ectopically express human Ace2

Depositor

Caroline Goujon

Article

Rebendenne et al J Virol. 2021 Jan 29. pii: JVI.02415-20.

Insert

human Ace2 (ACE2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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human rSHIP2 (18-893)

Plasmid

#128568

Purpose

Expression of human SHIP2 in bacteria

Depositor

Christophe Erneux

Article

Pesesse et al FEBS Lett. 1998 Oct 23;437(3):301-3.

Insert

SHIP2 (INPPL1 Human)

Tags

His Xpress (N-terminal)

Expression

Bacterial

Mutation

SHIP2 coding sequence from amino acid 18 (A) till…

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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Sumo3 PolQM1 deltains1

Plasmid

#78461

Purpose

Inducible expression in E. coli

Depositor

Sylvie Doublie, Susan Wallace

Article

Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

Insert

Polymerase theta (POLQ Human)

Tags

His and SUMO3

Expression

Bacterial

Mutation

delta 1-1791; delta 2149-2170; Q2513R (please see…

Promoter

T7

Available Since

July 28, 2016

Availability

Academic Institutions and Nonprofits only
NCKX4 scFv [N414/25]

Plasmid

#190553

Purpose

Mammalian Expression of NCKX4 scFV. Derived from hybridoma N414/25.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

NCKX4 (Homo sapiens) recombinant scFV (Slc24a4 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-ALDOA_sgRNA2

Plasmid

#201591

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

ALDOA (ALDOA Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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HOXB6 (human) FLAG pMSCV

Plasmid

#8534

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB6 (HOXB6 Human)

Use

Retroviral

Tags

FLAG and IRES-GFP

Expression

Mammalian

Mutation

contains improved ribosomal binding site and FLAG…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pSUMO-HsSyx3

Plasmid

#179288

Purpose

E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + SUMO + TEV protease cleavage site

Depositor

Debra Hansen

Article

Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

Insert

Syx

Tags

His6 + SUMO + TEV protease cleavage site

Expression

Bacterial

Mutation

residues 393-792 from accession number NP_0010361…

Promoter

T7

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAF138

Plasmid

#135923

Purpose

All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mCitrine fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).

Depositor

Andrew S. Flies

Article

Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

Insert

TNFRSF9 fused to mCitrine

Use

Transposon

Tags

mCitrine

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Synaptotagmin-6 scFv [N270/47]

Plasmid

#190544

Purpose

Mammalian Expression of Synaptotagmin-6 scFV. Derived from hybridoma N270/47.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Synaptotagmin-6 (Mus musculus) recombinant scFV (Syt6 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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TrpV1 scFv [N221/17]

Plasmid

#182082

Purpose

Mammalian Expression of TrpV1 scFv. Derived from hybridoma N221/17.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting TrpV1 (Rattus norvegicus) (Trpv1 Mouse)

Tags

Sortase, His

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pMT-ubb-Avi-Cerulean-RanGAP

Plasmid

#79881

Purpose

Ubiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)

Tags

Avi

Expression

Bacterial

Promoter

Ubiquitin promoter

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Coff/Fon-bREACHes-EYFP

Plasmid

#137147

Purpose

Intersectional viral expression of bREACHes-EYFP in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Insert

Coff/Fon-bREACHes-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-TrpV3 [N15/4R]

Plasmid

#177501

Purpose

Mammalian Expression Plasmid of anti-TrpV3 (Rat). Derived from hybridoma N15/4.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-TrpV3 (Rattus norvegicus) recombinant mouse monoclonal antibody (Trpv3 Rat)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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