We narrowed to 13,600 results for: sequence
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Plasmid#65853PurposePuromycin-based homologous recombination-dependent reporter for genome editing with TALENs or CRISPR/Cas9DepositorInsertsplit puromycin N-acetyltransferase coding sequence
UseCRISPR and TALENExpressionMammalianPromoterSV40Available SinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
AiRTX_ctrl
Plasmid#214069PurposeFluorescent reporter system, NMD negative control. Intron present in 5'UTRDepositorInsertTurboRFP
ExpressionMammalianMutationRemnant of linker sequence present at the end of …Available SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiRT
Plasmid#214067PurposeFluorescent reporter system to measure EJC-independent NMD activity. Intron present in 5'UTRDepositorInsertTurboRFP
ExpressionMammalianMutationLinker sequence present between RFP and GFPAvailable SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
p8.9 NdeltaSB D185K/D186L
Plasmid#132931Purposep8.9 NdeltaSB bearing RT-D185K/D186L mutant reverse transcriptase sequenceDepositorInsertgag and pol D185K/D186L
UseLentiviralMutationD185K/D186LAvailable SinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pREP
Plasmid#51491PurposepMDC32 with Rep/RepA coding sequence from the mild bean yellow dwarf virusDepositorInsertRep/RepA
UsePlant expression t-dnaMutationnonePromoter2x35SAvailable SinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
LiRTX_ctrl
Plasmid#214077PurposeLuminescent reporter system, NMD negative control. Intron present in 5'UTRDepositorInsertFirefly luciferase
ExpressionMammalianMutationRemnant of linker sequence present at the end of …Available SinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-72:TFAM-mEGFP
Plasmid#159745PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human TFAMDepositorInsertTFAM Homology Arms with linker-mEGFP (TFAM Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available SinceOct. 13, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pT7-EGFP-C1-HsTAB182_K
Plasmid#146777PurposeMammalian Expression of HsTAB182DepositorInsertHsTAB182 (TNKS1BP1 Human)
ExpressionMammalianMutation2 silent mutations compared to the sequence given…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
SL2 GFP H2B
Plasmid#227717PurposePlasmid contains SL2 GFP H2B, with some nlp-51 homology sequence before, Amp ResistantDepositorInsertSL2 GFP H2B (nlp-51 Nematode)
UseCloning vectorAvailable SinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
MKbpAII
Plasmid#91689PurposeMMTV-LTR promoter/enhancer with rabbit beta-globin intron and bovine growth hormone poly-A sequence to enhance transgene expressionDepositorInsertMMTV LTR cloned into KbpA vector
ExpressionMammalianPromoterMMTV LTRAvailable SinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKT-iRFP-KAN
Plasmid#64687PurposepKT vector encoding yeast-codon optimized infrared fluorescent protein iRFP.DepositorInsertiRFP
ExpressionYeastMutationNucleotide sequence has been yeast-codon optimize…Available SinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pT7 LasI
Plasmid#193631PurposeConstitutive T7 RNAP-mediated expression of LasI 3OC12-HSL synthase for Las quorum sensing system. LasI sequence is codon optimized for E. coli.DepositorInsertLasI
Tags6xHisExpressionBacterialPromoterT7 RNAP promoterAvailable SinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSCB2-sgRNA
Plasmid#129463PurposeTemplate plasmid for guide sequence insertion via inverse PCR. Derived from pSCrhaB2-gRNA (see paper).DepositorInsertpgRNA
UseCRISPRExpressionBacterialPromoterBBa_J23119 (SpeI)Available SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMC-J6-dCas9-10XGP41-J9
Plasmid#202005PurposeMoClo level 0 dCas9-10XGP41 coding sequence moduleDepositorInsertdCas9-10XGP41
UseSynthetic Biology; Moclo cloning vectorTagsGS linkersAvailable SinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTR47m4
Plasmid#104068PurposeLuciferase Reporter for Formaldehyde SensingDepositorInsertsxluxAB
frmR
frm Promoter m4
UseLuciferase and Synthetic BiologyExpressionBacterialMutation23r and 25th nucleotides in sequence mutated from…Available SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
AiRT_RTctrl
Plasmid#214070PurposeFluorescent reporter system, readthrough control, NMD negative control. Intron present in 5'UTRDepositorInsertTurboRFP
ExpressionMammalianMutationLinker sequence present between RFP and GFPAvailable SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2-Stuffer-HepmCherry
Plasmid#192825PurposeContains stuffer sequence into which sgRNAs can be cloned and expresses mCherry from hepatocyte-specific promoterDepositorInsertmCherry
UseCRISPR and LentiviralExpressionMammalianPromoterHepatocyte-specific promoter (HS-CRM8-TTRmin modu…Available SinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL2Basic-EcadK1/EpaIMUT/EboxMUT/Ebox2MUT
Plasmid#19291DepositorInsertE-cadherin
UseLuciferaseExpressionMammalianMutationE-cad promoter sequences from -108 to +125 Ebo…Available SinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLV-mRFP1-Human UTROPHIN_1-261
Plasmid#225052PurposeExpress in mammalian cells the mRFP1 fused to residues 1-261 of Human Utrophin to detect filamentous actin in live, fix cels or tissues.DepositorInsertHuman Utrophin residues 1-261 and RFP1 (UTRN Human, Homo Sapiens)
UseLentiviralTagsHuman UTROPHIN 1-261 and mRFP1ExpressionMammalianMutationSequence is derived from ADDGENE Plasmid #26739-R…PromoterCMV promoter and enhancerAvailable SinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHACK(Gal4)-DONR(T2A-LexAGAD)
Plasmid#194769PurposeTo insert T2A-LexAGAD into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-LexAGAD transgene.DepositorInsertT2A-LexAGAD
UseCRISPRExpressionInsectAvailable SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only