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HA-NFAT1(4-460)-GFP

Plasmid

#11107

Purpose

Expresses mouse NFAT1 (amino acids 4-460) with HA and GFP tags in mammalian cells

Depositor

Anjana Rao

Article

Aramburu et al Science. 1999 Sep 24. 285(5436):2129-33.

Insert

NFAT1 (Nfatc2 Mouse)

Tags

3x HA and GFP

Expression

Mammalian

Mutation

Contains amino acids 4-460 of NFAT1; L78P compare…

Promoter

CMV

Available Since

May 31, 2006

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Nst-MCS

Plasmid

#80487

Purpose

donor vector for AAVS1 targeting (neomycin selection) with multiple cloning site

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Type

Empty backbone

Use

Donor vector (human)

Expression

Mammalian

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs-KDM4D

Plasmid

#188234

Purpose

Express human KDM4D in mammalian cells

Depositor

Young Tang

Article

Su et al Int J Mol Sci. 2021 Sep 28;22(19). pii:

Insert

Lysine demethylase 4D (KDM4D Human)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pFD116

Plasmid

#124769

Purpose

pFD116 carries dcas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector

Depositor

David Bikard

Article

Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

Inserts

Ptet
dcas9
PpflB sgRNA BsaI

Use

CRISPR

Available Since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_N_Strep_BIRC6

Plasmid

#197972

Purpose

Mammalian cell expression of wt Strep-BIRC6

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

BIRC6 (BIRC6 Human)

Tags

Strep

Expression

Mammalian

Mutation

L1332V

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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GST-TACC3-His6

Plasmid

#69106

Purpose

For bacterial expression of recombinant human TACC3 tagged with GST and His.

Depositor

Stephen Royle

Article

Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

Insert

Transforming Acidic Coiled Coil protein 3 (TACC3 Human)

Tags

GST and His6

Expression

Bacterial

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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Stat3-C Flag pRc/CMV

Plasmid

#8722

Depositor

Jim Darnell

Article

Bromberg et al Cell 1999 Aug 6;98(3):295-303.

Insert

Stat3-C (Stat3 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

A662C and N664C. Residues changed to Cys renders…

Available Since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

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pBS human IRS-1

Plasmid

#11359

Depositor

Ronald Kahn

Article

Araki et al Diabetes. 1993 Jul . 42(7):1041-54.

Insert

IRS-1 (IRS1 Human)

Expression

Bacterial

Available Since

March 1, 2006

Availability

Academic Institutions and Nonprofits only

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pJT11

Plasmid

#98308

Purpose

Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Citrus limon limonene synthase LIS1 under the control of GAL1/GAL2 promoter

Depositor

Claudia Vickers

Article

Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(

Insert

TRPL3ClLIS1>TRPL41B

Expression

Yeast

Available Since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pJT2

Plasmid

#98306

Purpose

Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoter

Depositor

Claudia Vickers

Article

Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(

Insert

PTEF2>ScERG20N127W>TRPL3>PTEF1>AcNES1>TRPL41B

Expression

Yeast

Mutation

ERG20 N127W

Available Since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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