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pCIS.MTRIA-PAF

Plasmid

#184616

Purpose

For expression of a fluorescent sensor for platelet-activating factor in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-PAF

Expression

Mammalian

Promoter

CAG promoter

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-UTS

Plasmid

#184620

Purpose

For expression of a fluorescent sensor for urotensin II in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-UTS

Expression

Mammalian

Promoter

CAG promoter

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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His8OphMADeltaC6-18mer.

Plasmid

#189671

Purpose

His8 tagged OphMADeltaC6 with TEV cleavable C-terminus to generate truncated 18merTr and 15mer peptides

Depositor

Markus Künzler, James Naismith

Article

Emmanuel et al bioRxiv 2022.07.21.500988

Insert

His8OphMA∆C6-TEV18mer

Tags

His8 tagged

Expression

Bacterial

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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LDB221

Plasmid

#185427

Purpose

Bacterial expression of Nup1 C-terminal residues 777-1076 as GST fusion

Depositor

Ken Belanger, Laura Davis

Article

Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25

Insert

NUP1

Tags

GST

Expression

Bacterial

Mutation

Nup1 C-terminal residues 777-1076

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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LDB203

Plasmid

#185424

Purpose

Bacterial expression of N-terminus of NUP1 nucleoporin as GST-Nup1-N fusion

Depositor

Ken Belanger, Laura Davis

Article

Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25

Insert

NUP1

Tags

GST

Expression

Bacterial

Mutation

Nup1 N-terminal domain only

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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LDB205

Plasmid

#185425

Purpose

Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion

Depositor

Ken Belanger, Laura Davis

Article

Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25

Insert

NUP1

Tags

GST

Expression

Bacterial

Mutation

Nup1 C-terminal domain only

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP231-Lenti-TRE3G-Flag-GluN2B(E1479Q)-WPRE-pA

Plasmid

#164796

Purpose

pLenti vector backbone designed to express Flag Tagged-GluN2B(E1479Q) from a TRE3G promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

GluN2B

Use

Lentiviral

Tags

Flag

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP226-Lenti-CMV-Flag-GluN2B-WRPE-pA

Plasmid

#84275

Purpose

pLenti vector backbone designed to express Flag-GluN2B from a CMV promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

GluN2B

Use

Lentiviral

Tags

Flag

Promoter

CMV

Available Since

July 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-LTB

Plasmid

#184605

Purpose

For expression of a fluorescent sensor for leukotriene B4 in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-LTB

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-NTS

Plasmid

#184614

Purpose

For expression of a fluorescent sensor for neurotensin in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-NTS

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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UPATrap-VC-ORF2

Plasmid

#110653

Purpose

transposon containing BiFC VC cassette

Depositor

Chris Lord

Article

Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

Insert

Venus C-terminus cassette

Expression

Mammalian

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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UPATrap-VC-ORF1

Plasmid

#110652

Purpose

transposon containing BiFC VC cassette

Depositor

Chris Lord

Article

Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

Insert

Venus C-terminus cassette

Expression

Mammalian

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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UPATrap-VC-ORF0

Plasmid

#110651

Purpose

transposon containing BiFC VC cassette

Depositor

Chris Lord

Article

Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

Insert

Venus C-terminus cassette

Expression

Mammalian

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-NMB

Plasmid

#184610

Purpose

For expression of a fluorescent sensor for neuromedin B in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-NMB

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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KBB101

Plasmid

#185064

Purpose

Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion truncated after the last FXFG repeat

Depositor

Ken Belanger

Article

Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25

Insert

NUP1

Tags

GST

Expression

Bacterial

Mutation

Nup1 C-terminal region, truncated

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pGWT35S-KON206-MXMT

Plasmid

#182729

Purpose

Transient expression of KON206-MXMT in plant cell

Depositor

Yutaka Kodama

Article

Fujii et al Biotechniques. 2018 Apr;64(4):153-161. d

Insert

KON206

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGWT35S-KOC169-MXMT

Plasmid

#182728

Purpose

Transient expression of KOC169-MXMT in plant cell

Depositor

Yutaka Kodama

Article

Fujii et al Biotechniques. 2018 Apr;64(4):153-161. d

Insert

KOC169

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGWT35S-KON154-MXMT

Plasmid

#182725

Purpose

Transient expression of KON154-MXMT in plant cell

Depositor

Yutaka Kodama

Article

Fujii et al Biotechniques. 2018 Apr;64(4):153-161. d

Insert

KON154

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pGWT35S-KOC155-MXMT

Plasmid

#182726

Purpose

Transient expression of KOC155-MXMT in plant cell

Depositor

Yutaka Kodama

Article

Fujii et al Biotechniques. 2018 Apr;64(4):153-161. d

Insert

KOC155

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pGWT35S-KON168-MXMT

Plasmid

#182727

Purpose

Transient expression of KON168-MXMT in plant cell

Depositor

Yutaka Kodama

Article

Fujii et al Biotechniques. 2018 Apr;64(4):153-161. d

Insert

KON168

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-Lyn-nTVMVpmut-tevs-AP4-tevs-P3-cTVMVp

Plasmid

#179672

Purpose

Membrane-bound (Lyn) TEVP-inducible caged-cTVMVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Caged cTVMVP

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-p450-hcvs-B2AD2-26Sfur-SEAP

Plasmid

#179647

Purpose

HCVP-inducible p450 SEAP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-SEAP-26Sfur-B2AD-SpyTag-tvmvs-KKMP

Plasmid

#179648

Purpose

TVMVP-inducible single TM SEAP RELEASE construct with SpyTag

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-nTVMVp-AP4-tevs-P3-tevs-cTVMVPmut-CAAX

Plasmid

#179662

Purpose

Membrane-bound (CAAX) TEVP-inducible caged-nTVMVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Caged-nTVMVP

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-GFP-ECL-3TM-hcvs-KKMP

Plasmid

#179641

Purpose

HCVP-inducible membrane-bound GFP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Membrane-bound GFP RELEASE

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-SEAP-26sFur-B2AD-hcvs-tevs-KKMP

Plasmid

#179643

Purpose

OR Gate (TEVP/HCVP) SEAP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJEP206-AAV-1.3CaMKII-MCS-pA.

Plasmid

#78319

Purpose

AAV vector backbone containing a 1.3CaMKIIa promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA)

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Type

Empty backbone

Use

AAV

Promoter

1.3CaMKIIa

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP225-Lenti-CMV-Flag-GluN2A-WPRE-pA

Plasmid

#84274

Purpose

pLenti vector backbone designed to express Flag-GluN2A from a CMV promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Flag-GluN2A

Use

Lentiviral

Tags

Flag

Promoter

CMV

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJEP202-Lenti-TRE3G-GFPGluN2A-WPRE-pA

Plasmid

#164776

Purpose

pLenti vector backbone designed to express GFP-GluN2A from a TRE3G promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

GluN2A

Use

Lentiviral

Tags

GFP

Promoter

1.3αCaMKII

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP210-AAV-0.4CaMKII P-MCS-pA

Plasmid

#63084

Purpose

AAV2 vector backbone containing a 0.4CaMKIIa promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA)

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Type

Empty backbone

Use

AAV

Promoter

0.4αCaMKII

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge