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pAAV-EFS-CasRx-pA

Plasmid

#192482

Purpose

To express CasRx

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

EFS

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-0.4CaMKII-CasRx-T2A-mCherry-pA

Plasmid

#192483

Purpose

To express CasRx

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

0.4CaMKIIa

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-CasRx-T2A-mCherry-pA

Plasmid

#192484

Purpose

To express CasRx

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

Ef1a

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-intron-CasRx-pA

Plasmid

#192487

Purpose

To express CasRx

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

CMV

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-hfCas13d-pa

Plasmid

#233037

Purpose

To express hfCas13d from a EF1a promoter

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

hfCas13d

Use

AAV

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pFUGW hUbC-Sga-dCas9-KRAB-T2A-GFP

Plasmid

#227009

Purpose

Lentiviral Expression of dCas9-KRAB from S. gallolyticus fused to the repressive KRAB domain

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xFLAG, SV40 NLS

Promoter

UbC

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pFUGW hUbC-Sin-dCas9-KRAB-T2A-GFP

Plasmid

#227010

Purpose

Lentiviral Expression of dCas9-KRAB from S. iniae fused to the repressive KRAB domain

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xFLAG, SV40 NLS

Promoter

UbC

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pFUGW hUbC-Spa-dCas9-KRAB-T2A-GFP

Plasmid

#227011

Purpose

Lentiviral Expression of dCas9-KRAB from S. parasanguinis fused to the repressive KRAB domain

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xFLAG, SV40 NLS

Promoter

UbC

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pFUGW hUbC-Sub-dCas9-KRAB-T2A-GFP

Plasmid

#227012

Purpose

Lentiviral Expression of dCas9-KRAB from S. uberis fused to the repressive KRAB domain

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xFLAG, SV40 NLS

Promoter

UbC

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF13AS1371D

Plasmid

#224417

Purpose

Mammalian expression of KIF 13a S1371D

Depositor

Marvin Bentley

Article

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

KIF 13a S1371D (Kif13a Mouse)

Tags

GFP

Expression

Mammalian

Mutation

S1371D

Promoter

Chicken Beta actin

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pIN3 (gpdA-cTerm-Citrine2-ptrA)

Plasmid

#188737

Purpose

Expresses Citrine2 for C-terminal tagging

Depositor

Michael Bromley

Article

Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

Insert

gdpA-Citrine2 PtrA

Use

Filamentous fungal expression

Tags

Citrine2

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pIN4 (gpdA-nTerm-Citrine2-ptrA)

Plasmid

#188738

Purpose

Expresses Citrine2 for N-terminal tagging

Depositor

Michael Bromley

Article

Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

Insert

gdpA-Citrine2 PtrA

Use

Filamentous fungal expression

Tags

Citrine2

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pIN5 (gpdA-cTerm-mTurqoise2-ptrA)

Plasmid

#188739

Purpose

Expresses mTurqoise2 for C-terminal tagging

Depositor

Michael Bromley

Article

Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

Insert

gdpA-mTurquoise PtrA

Use

Filamentous fungal expression

Tags

mTurquoise

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pIN6 (gpdA-nTerm-mTurqoise2-ptrA)

Plasmid

#188740

Purpose

Expresses mTurqoise2 for N-terminal tagging

Depositor

Michael Bromley

Article

Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

Insert

gdpA-mTurqoise2 PtrA

Use

Filamentous fungal expression

Tags

mGreenlantern

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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TL1 alphaDdCBE A1 (DddA6-C) - TL1 alphaL_DdCBE 2 (left, DddA11-C)

Plasmid

#221994

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE A1 repeats-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

TALE: Q231R, W232G, S233A | DddA: T1413I

Promoter

CMV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF13AS1371A

Plasmid

#224409

Purpose

Mammalian expression of KIF 13a S1371A

Depositor

Marvin Bentley

Article

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

KIF 13a S1371A (Kif13a Mouse)

Tags

GFP

Expression

Mammalian

Mutation

S1371A

Promoter

Chicken Beta actin

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF13AWLDLE

Plasmid

#224410

Purpose

Mammalian expression of KIF 13a WLDLE

Depositor

Marvin Bentley

Article

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

KIF 13a WLDLE (Kif13a Mouse)

Tags

GFP

Expression

Mammalian

Mutation

WLDLE

Promoter

Chicken Beta actin

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pET28Man

Plasmid

#203399

Purpose

exo-beta mannosidase useful in enzymatic biotransformations of glycans and in coupled assays of various carbohydrate processing enzymes

Depositor

David Vocadlo

Article

Deng et al Bioorg Med Chem. 2013 Aug 15;21(16):4839

Insert

man2A

Tags

H6

Expression

Bacterial

Mutation

None

Promoter

T7

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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Src(YF)-SsrA(Y7I)-mCer3

Plasmid

#220999

Purpose

this is microTag (Y7I mutation in SsrA)-Src(Y529F) with mCerulean3 at C-terminal

Depositor

Klaus Hahn

Article

Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

Insert

Src, SsrA, mCerulean3 (Src Mouse)

Tags

mCerulean3

Expression

Mammalian

Mutation

Y529F in Src, Y7I in SsrA

Promoter

CMV

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMX1463

Plasmid

#208805

Purpose

Expresses plasma-membrane-bound FRB-SBFP2 for rapid relocalization of cytoplasmic proteins tagged with FKBP upon addition of rapamycin

Depositor

Linda Wordeman

Article

Wordeman et al Mol Biol Cell. 2016 Apr 15;27(8):1300-9.

Insert

FRB (CRP Human)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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