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pEAHISMRSATAG

Plasmid

#97004

Purpose

Expressing MRSA TAG protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

DNA-3-methyladenine glycosylase I

Tags

N-ter TEV protease cleavable 6His

Promoter

T7

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP eIF2beta C-term (aa 89-331)

Plasmid

#45901

Depositor

Louise Larose

Article

Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

Insert

eIF2S2 aa89-331 (Eif2s2 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

aa 89-331

Promoter

CMV

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pSV HA eIF2beta (Pro110-113)

Plasmid

#45899

Depositor

Louise Larose

Article

Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

Insert

eIF2beta (Eif2s2 Mouse)

Tags

HA

Expression

Mammalian

Mutation

P110A, P113A

Promoter

SV40

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
pGY037 BSMV-gamma-AmCyan

Plasmid

#234818

Purpose

To express flourescent protein AmCyan from the gamma genome of Barley Stripe Mosaic Virus Vector (T-DNA)

Depositor

Daniel Voytas

Article

Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

Insert

AmCyan

Expression

Plant

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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HiBiT-FZD2

Plasmid

#195840

Purpose

Expresses human FZD2 with an N-terminal HiBiT

Depositor

Gunnar Schulte

Article

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD2 (FZD2 Human)

Tags

HiBiT

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-EBNA3B

Plasmid

#198574

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

EBNA3B (EBNA-3B/EBNA-3C Epstein-Barr virus)

Use

Gateway Cloning

Mutation

None (wildtype)

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-EBNA3A

Plasmid

#198575

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

EBNA3A (EBNA-3A Epstein-Barr virus)

Use

Gateway Cloning

Mutation

None (wildtype)

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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HiBiT-FZD10

Plasmid

#195848

Purpose

Expresses human FZD10 with an N-terminal HiBiT

Depositor

Gunnar Schulte

Article

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD10 (FZD10 Human)

Tags

HiBiT

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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HiBiT-FZD4

Plasmid

#195842

Purpose

Expresses human FZD4 with an N-terminal HiBiT

Depositor

Gunnar Schulte

Article

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD4 (FZD4 Human)

Tags

HiBiT

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pY1

Plasmid

#50597

Purpose

Backbone: pRBS-01, Origin Of Replication: p15a, Selection Markers: Amp, Promoters: PLacUV5,

Depositor

Jay Keasling

Article

Juminaga et al Appl Environ Microbiol. 2012 Jan;78(1):8

Type

Empty backbone

Use

Synthetic Biology; Empty vector

Expression

Bacterial

Promoter

PLacUV5

Available Since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only
HSC1-D4Z4-GiP

Plasmid

#58542

Purpose

Derived from HSC1-GiP where the D4Z4 insulator is cloned upstream of the EF1 alpha promoter

Depositor

James Ellis

Article

Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

Inserts

Enhanced Green Fluorescence Protein, Puromycin resistance gene
D4Z4 repeat, partial

Use

Retroviral

Expression

Mammalian

Promoter

Human EF1-alpha

Available Since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV4-Flag-mIL-17RA/YFP.Hygro

Plasmid

#46862

Purpose

encodes Flag-tagged mouse IL-17RA fused to Yellow Fl. Protein

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

IL-17RA-YFP

Tags

Flag/IL-3 signal sequence and fused to CFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only
pT7-EGFP-C1-HsXRN1_1-1173_K

Plasmid

#146785

Purpose

Mammalian Expression of HsXRN1_1-1173

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

HsXRN1_1-1173 (XRN1 Human)

Expression

Mammalian

Mutation

one silent mutation compared to the sequence give…

Available Since

Nov. 11, 2020

Availability

Academic Institutions and Nonprofits only

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p123shy1-mCherry-HA

Plasmid

#67875

Purpose

encodes shy1 with a C-terminal mCherry-HA tag under control of its endogenous promoter.It's suitable for localization studies in Ustilago maydis.

Depositor

Armin Djamei

Article

Rabe et al Mol Microbiol. 2013 Jul;89(1):179-88. do

Insert

shy1

Use

Expression in ustilago maydis

Tags

HA and mCherry

Promoter

shy1

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV4Neo-murine IL-17RASEFIR-HA

Plasmid

#46863

Purpose

expresses mouse IL-17RA with SEFIR deletion

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

IL-17RA-delta-SEFIR (Il17ra Mouse)

Tags

3x HA

Expression

Mammalian

Mutation

Deletion of SEFIR domain (AA 394-485)

Promoter

CMV

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only
pCMV-AID-PcrA-UGI

Plasmid

#221220

Purpose

Express HACE Editor as a fusion of AID cytidine deaminase and PcrA helicase.

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

AID-PcrA-UGI

Expression

Mammalian

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_CnCas12f1

Plasmid

#204996

Purpose

CnCas12f1 heterologous expression plasmid

Depositor

Quanjiang Ji

Article

Su et al Nat Chem Biol. 2024 Feb;20(2):180-189. d

Insert

CnCas12f1

Use

CRISPR

Promoter

T7

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG1.1-TSSK2/3xFLAG

Plasmid

#199625

Purpose

Expression vector of mouse TSSK2. 3xFLAG tag at C-terminus.

Depositor

Masahito Ikawa

Article

Shimada et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

Insert

Tssk2 mRNA (Tssk2 Mouse)

Tags

3xFLAG

Promoter

CAG

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pEF6 Vps54-V5-6His

Plasmid

#175098

Purpose

V5 tagged Vps54 is expressed in mammalian cells

Depositor

Juan Bonifacino

Article

Perez-Victoria et al Mol Biol Cell. 2008 Jun;19(6):2350-62. d

Insert

Vps54 (Vps54 Mouse)

Tags

V5

Expression

Mammalian

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19/mlcR25-mEGFP

Plasmid

#154295

Purpose

Fluorescent reporter for conony screening without an inducer (e.g. IPTG); TED module (Plac-lacO-mlcR25-mEGFP)

Depositor

Shigehiko Yumura

Article

Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

Insert

25-bp fragment of the 3′ end of mlcR (mlcR25); mEGFP

Expression

Bacterial

Available Since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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