We narrowed to 22,524 results for: Sis

1. pHR-mCh-Cry2olig

   Plasmid

   #101222

   Purpose

   Cry2olig(E490G)

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

   Insert

   Cry2olig
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   E490G

   Promoter

   SFFV

   Available since

   Feb. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKS1

   Plasmid

   #84079

   Purpose

   Expresses the non-toxic fragment C of tetanus toxin

   Depositor

   Neil Fairweather

   Article

   Sinha et al Mol Microbiol. 2000 Sep;37(5):1041-51.

   Insert

   Tetanus neurotoxin fragment C
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSGKP-spe

   Plasmid

   #117234

   Purpose

   A sgRNA expression plasmid for genome editing in Klebsiella Pneumoniae

   Depositor

   Quanjiang Ji

   Article

   Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV-GFP-NMHC II-B

   Plasmid

   #11348

   Depositor

   Robert Adelstein

   Article

   Wei et al Mol Biol Cell. 2000 Oct . 11(10):3617-27

   Insert

   NMHC II-B (MYH10 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMCB320

   Plasmid

   #89359

   Purpose

   3rd generation lentiviral sgRNA expression vector with mCherry, puromycin resistance, BlpI+BstXI cloning sites

   Depositor

   Michael Bassik

   Article

   Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

   Insert

   puromycin resistance, mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS41K-mCherry-sfGFP (pJJF001)

   Plasmid

   #173457

   Purpose

   C-SWAT donor for Seamless tagging, contains mCherry-sfGFP tandem fluorescent protein timer

   Depositor

   Anton Khmelinskii

   Article

   Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

   Insert

   mCherry-sfGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   no promoter

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330S-2-PITCh

   Plasmid

   #63670

   Purpose

   Expresses Cas9 nuclease and the PITCh-gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. Mouse 3' Ubf AID GFP PuroR

   Plasmid

   #127901

   Purpose

   HDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Mouse Ubf Gene

   Depositor

   Mark Groudine

   Insert

   Ubf (Ubtf Mouse, Mustard Weed)
   

   Use

   Donor plasmid

   Tags

   GFP, AID, PuroR

   Expression

   Mutation

   Inserting GFP AID 2A Puro into mouse 3' Ubf

   Promoter

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRIS-PITChv2-FBL

   Plasmid

   #63672

   Purpose

   PITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locus

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

   Insert

   EGFP-2A-PuroR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Promoterless

   Available since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV5-hPPT1

    Plasmid

    #85815

    Purpose

    Expression of hPPT in mammalian cells

    Depositor

    Sandra Hofmann

    Article

    Lu et al Mol Genet Metab. 2010 Apr;99(4):374-8. d

    Insert

    human PPT1 (PPT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGFP-Rab8A[Q67L]

    Plasmid

    #24900

    Depositor

    Maxence Nachury

    Article

    Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

    Insert

    hRab8A[Q67L]
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed Glutamine 67 to Leucine

    Promoter

    Available since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGFP-Rab8A[T22N]

    Plasmid

    #24899

    Depositor

    Maxence Nachury

    Article

    Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

    Insert

    hRab8A[T22N]
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed Threonine 22 to Asparagine

    Promoter

    Available since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMTU-DO-HPH

    Plasmid

    #160008

    Purpose

    Low-copy expression vector for K. marxianus, hygromycin selection

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET16B.Pfu

    Plasmid

    #12509

    Purpose

    Bacterial expression of Pfu DNA polymerase

    Depositor

    Sung-Hou Kim

    Article

    Goldman et al Acta Crystallogr D Biol Crystallogr. 199

    Insert

    Pfu DNA polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y218H, Q462R, F533L, K559I and V683A (mutations w…

    Promoter

    Available since

    Sept. 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBabe-c-myc-zeo

    Plasmid

    #17758

    Depositor

    Susan Lindquist

    Article

    Dai et al Cell. 2007 Sep 21. 130(6):1005-18.

    Insert

    c-myc (MYC Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMTU-DO-G418-C4

    Plasmid

    #160342

    Purpose

    High-copy expression vector for K. marxianus, G418 selection

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLminP_Luc2P_RE1

    Plasmid

    #90335

    Purpose

    NFkB - p50/p65 pathway gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    NFKB

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAC-BETAipi

    Plasmid

    #53277

    Purpose

    Contains ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Plant J. 2005 Feb;41(3):478-92.

    Insert

    idi
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    endogenous promoter

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 phiC31

    Plasmid

    #68310

    Purpose

    In vitro transcription vector to generate phiC31 mRNA (i.e. for zebrafish injection).

    Depositor

    Konrad Basler

    Article

    Bischof et al Proc Natl Acad Sci U S A. 2007 Feb 27;10

    Insert

    phiC31
    

    Use

    Rna transcription

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. HyPer7.2DAAO-mito

    Plasmid

    #168304

    Purpose

    Chemogenetic fluorescent H2O2 reporter targeted to the mitochondria

    Depositor

    Thomas Michel

    Article

    Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

    Insert

    HyPer7.2DAAO-mito
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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