We narrowed to 16,131 results for: form

1. actc1b-mKate2-rab7b

   Plasmid

   #109656

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to rab7b

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   rab7b (rab7b Zebrafish)
   

   Use

   Zebrafish plasmids

   Tags

   mKate2

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. KG802: pMVP (L3-L2) V5 epitope tag + polyA

   Plasmid

   #121755

   Purpose

   pMVP L3-L2 entry plasmid, contains V5 epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   V5 epitope tag + polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGex-3x Flag MKK3

   Plasmid

   #14814

   Depositor

   Roger Davis

   Article

   Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

   Insert

   MKK3 (MAP2K3 Human)
   

   Tags

   Flag

   Expression

   Bacterial

   Available Since

   July 25, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. rGSKmyc-R85-pXT7

   Plasmid

   #15493

   Depositor

   Sergei Sokol

   Article

   Dominguez et al Proc Natl Acad Sci U S A. 1995 Aug 29. 9

   Insert

   GSK-3 beta R85 (Gsk3b Rat)
   

   Use

   Xenopus rna expression

   Tags

   myc

   Mutation

   R85: K85R, dominant negative

   Available Since

   Aug. 1, 2007

   Availability

   Academic Institutions and Nonprofits only

5. actc1b-mKate2-rab8a

   Plasmid

   #109657

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to rab8a

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   rab8a (rab8a Zebrafish)
   

   Use

   Zebrafish plasmids

   Tags

   mKate2

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLib6.4-Aalb_726

   Plasmid

   #176661

   Purpose

   Expression of sgRNA under Ae. albopictus U6 (AALF029726) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Expression

   Insect

   Available Since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLib6.4-Aaeg_763

   Plasmid

   #176658

   Purpose

   Expression of sgRNA under Ae. Aegypti U6 (AAEL017763) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Expression

   Insect

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. 5xtetO (tet3) Gene Desert

   Plasmid

   #131339

   Purpose

   gRNA to insert 5xTet operator sequence into a gene desert on chromosome 5.

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   Desert-gRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUPD_OsU3 (GB1184)

   Plasmid

   #68209

   Purpose

   Provides the O. sativa U3 RNA polIII promoter as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   OsU3 promoter
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSG5-Myr-FLAG-p110β-DM

    Plasmid

    #55719

    Purpose

    eukaryotic expression of myristoylation tag (Myr-FLAG) p110b RBD double mutant S205D, K224A

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    p110 beta
    

    Tags

    myristoylation tag (Myr-FLAG)

    Expression

    Mammalian

    Mutation

    S205D, K224A

    Promoter

    T7

    Available Since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pCLC3sFlag

    Plasmid

    #52422

    Purpose

    mammalian expression of flag tagged rat CLC3 short transcript

    Depositor

    Steven Weinman

    Article

    Shimada et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    CLC3 (Clcn3 Rat)
    

    Tags

    flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPAtUbq10 (GB0223)

    Plasmid

    #68174

    Purpose

    Provides the Arabidopsis thaliana polyubiquitin 10 promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtUbq10 promoter
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. JN402: pMVP (R4-R3) Firefly Luciferase

    Plasmid

    #121741

    Purpose

    pMVP R4-R3 entry plasmid, contains Firefly Luciferase for 3- or 4-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Firefly Luciferase
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. KW701: pMVP (L5-L4) AID

    Plasmid

    #121723

    Purpose

    pMVP L5-L4 entry plasmid, contains AID for 4-component MultiSite Gateway Pro assembly. Allows fusion of minimal N-term Auxin Induced Degron (AID) domain (activated by auxin) to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    AID (open)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-RELΔ424-490

    Plasmid

    #27267

    Depositor

    Thomas Gilmore

    Article

    Starczynowski et al Oncogene. 2007 Apr 26. 26(19):2685-94.

    Insert

    REL (REL Human)
    

    Expression

    Mammalian

    Mutation

    Deletion of transactivation domain I (aa 425-490)

    Available Since

    Feb. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

16. II901: pMVP (L5-L4) FKBP DD

    Plasmid

    #121720

    Purpose

    pMVP L5-L4 entry plasmid, contains FKBP DD for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term FKBP degron domain (stabilized by SHLD1) to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    FKBP Degron Domain (open)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. UAS-Xbp1-GFP

    Plasmid

    #80627

    Purpose

    expression of Xbp1-GFP (RA isoform) in Drosophila

    Depositor

    Pedro Domingos, Herman Steller

    Article

    Ryoo et al EMBO J. 2007 Jan 10;26(1):242-52. Epub 2

    Insert

    Xbp1 (Xbp1 Fly)
    

    Tags

    EGFP

    Expression

    Insect

    Mutation

    RA isoform

    Promoter

    UAS

    Available Since

    July 28, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pCellFree_G03 CSNK2A1

    Plasmid

    #67085

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CSNK2A1 (CSNK2A1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pGSTag Flag Jnk3a1(2nd methione)

    Plasmid

    #15746

    Depositor

    Roger Davis

    Article

    Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

    Insert

    Jnk3a1-2nd M (MAPK10 Human)
    

    Expression

    Bacterial

    Available Since

    March 6, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pEM01

    Plasmid

    #135414

    Purpose

    Expression of Hygromycin resistance from CMV promoter

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    CMVpr hph ADH1t
    

    Expression

    Bacterial and Yeast

    Promoter

    CMVpr

    Available Since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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