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pDEST-CMV-polysite

Plasmid

#122843

Purpose

Gateway destination vector for mammalian expression without tag, or for adding custom tags by conventional cloning. Distinct multiple cloning sites 5' and 3' of gateway cassette.

Depositor

Robin Ketteler

Publication

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Gateway Cloning

Expression

Mammalian

Promoter

CMV

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3-RAB-EKARev

Plasmid

#118474

Purpose

ddRFP-based single-color biosensor for monitoring ERK activity.

Depositor

Jin Zhang

Publication

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-EKARev

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available since

April 30, 2019

Availability

Academic Institutions and Nonprofits only

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CLYBL-TO-hNIL-BSD-mApple

Plasmid

#124230

Purpose

Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2-ISL1-LHX3 for differentiation of iPSCs into lower motor neurons. Contains a floxed BSD-NLS-mApple selection cassette.

Depositor

Michael Ward

Insert

NGN2, ISL1, LHX3 (NEUROG2, ISL1, LHX3 Human)

Expression

Mammalian

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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SrtA-PDGFR Tm

Plasmid

#121168

Purpose

pMP71 vector expressing Tomato - P2A - flag - SrtA - PDGFR Tm

Depositor

Gabriel Victora

Publication

Pasqual et al Nature. 2018 Jan 25;553(7689):496-500. d

Insert

SrtA-PDGFR

Use

Retroviral

Tags

Tomato and flag

Expression

Mammalian

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSP2-96-merTetO-EFS-BLaR

Plasmid

#118713

Purpose

Contains an optimized 96-mer TetO repeat for imaging of desired loci

Depositor

Huimin Zhao

Publication

Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

Inserts

Blasticidin resistance gene
EFS-NS promoter

Expression

Mammalian

Promoter

EFS-NS

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLNCX2-mEGFP-VPS4A

Plasmid

#116924

Purpose

expresses mEGFP-VPS4A in mammalian cells

Depositor

Sanford Simon

Publication

Bleck et al Proc Natl Acad Sci U S A. 2014 Aug 19;11

Insert

VPS4A (VPS4A Human)

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Mutation

silent mutation in position 4059 C to T

Promoter

CMV

Available since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pHR CD19-mMegf10 CAR

Plasmid

#113013

Purpose

for production of lentivirus encoding the CD19 CAR-Pmegf10

Depositor

Ron Vale

Publication

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

CD19 CAR-P Megf10 intracellular domain (CD19, Megf10 Human, Synthetic, Mouse)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti6.3/TO/CD9-GFP

Plasmid

#104402

Purpose

Expresses CD9 with mEmerald tag (NOT GFP) at the N terminal

Depositor

Jens Gruber

Publication

Boker et al Mol Ther. 2018 Feb 7;26(2):634-647. doi:

Insert

CD9 (CD9 Human)

Use

Lentiviral

Tags

mEmerald

Promoter

CMV

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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LLP252 pEF1a-NLS-scFvGCN4-DNMT3a

Plasmid

#100941

Purpose

Single chain variable fragment antibody GCN4 fused to DNMT3a, binds SunTag, with 3xTy1 tag

Depositor

Ryan Lister

Publication

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

scFvGCN4, sfGFP, GB1, DNMT3a catalytic domain (DNMT3A Human, Synthetic)

Tags

1xHA and 3xTy1

Expression

Mammalian

Mutation

D10A, H840A for dCas9

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pDNA2.0 6H-TEV-KRAS

Plasmid

#111849

Purpose

Expresses wild type human KRAS (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.

Depositor

Kenneth Westover

Publication

Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.

Insert

KRAS (KRAS Human)

Tags

6xHis-tag and TEV protease cleavage sequence

Expression

Bacterial

Promoter

T5

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV-CWB-cyan pre-eGRASP(p32)

Plasmid

#111579

Purpose

An AAV vector that expresses cyan pre-eGRASP(p32) under the CaMKIIa promoter.

Depositor

Bong-Kiun Kaang

Publication

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

cyan pre-eGRASP(p32)

Use

AAV

Promoter

CaMKIIa

Available since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDawn-sfGFP

Plasmid

#107741

Purpose

Expresses superfolder GFP under the control of a blue-light transcriptional regulator

Depositor

Ingmar Riedel-Kruse

Publication

Jin et al Proc Natl Acad Sci U S A. 2018 Apr 3;115

Insert

superfolder GFP

Expression

Bacterial

Promoter

pLambda

Available since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAG-Archon1-KGC-EGFP-ER2-WPRE

Plasmid

#108423

Purpose

Archon1 fluorescent voltage reporter in mammalian expression plasmid

Depositor

Edward Boyden

Publication

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

Archon1-KGC-EGFP-ER2

Expression

Mammalian

Promoter

CAG

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pGP-AAV-syn-jGCaMP7b-WPRE

Plasmid

#104489

Purpose

AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter

Depositor

Douglas Kim, GENIE Project

Publication

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Has service

AAV1 and AAV Retrograde

Insert

jGCaMP7b

Use

AAV

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

Synapsin

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pcDNA3.1 Flag-HIF1B (#930)

Plasmid

#99916

Purpose

mammalian expression

Depositor

James Brugarolas

Publication

Chen et al Nature. 2016 Nov 3;539(7627):112-117. do

Insert

HIF1B (ARNT Human)

Tags

FLAG

Expression

Mammalian

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCold-I-Dsup

Plasmid

#90021

Purpose

To express His-tagged Dsup in bacteria

Depositor

Takekazu Kunieda

Publication

Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

Insert

Dsup

Tags

His

Expression

Bacterial

Promoter

cspA

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDONR223_PIK3CA_p.H1047R

Plasmid

#82824

Purpose

Gateway Donor vector containing PIK3CA, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PIK3CA (PIK3CA Human)

Use

Gateway Cloning

Mutation

H1047R

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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TetO-FUW-pgk-puro

Plasmid

#85747

Purpose

tetracyline-inducible lentiviral vector with no insert for use in mammalian cells

Depositor

Emily Dykhuizen

Publication

Chowdhury et al PLoS One. 2016 Apr 21;11(4):e0153718. do

Type

Empty backbone

Use

Lentiviral

Tags

none

Promoter

CMV

Available since

March 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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sgOpti

Plasmid

#85681

Purpose

Lentiviral vector to express sgRNAs. The cloning site is BsmBI.

Depositor

Eric Lander, David Sabatini

Publication

Fulco et al Science. 2016 Sep 29. pii: aag2445.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

28 Articles

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Lenti-AsCpf1-Blast

Plasmid

#84750

Purpose

Expresses human codon-optimized AsCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

Depositor

Hyongbum Kim

Publication

Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

Insert

AsCpf1

Use

CRISPR and Lentiviral

Tags

NLS-3xHA (C terminal on insert)

Expression

Mammalian

Promoter

EFS-NS

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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