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pORE303N_CEN1

Plasmid

#194439

Purpose

CRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout CEN1

Depositor

Chung-Jui Tsai

Article

Ortega et al New Phytol. 2022 Nov 16. doi: 10.1111/np

Insert

gRNA targeting CEN1

Use

CRISPR

Expression

Plant

Promoter

MtU6

Available Since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF

Plasmid

#177267

Purpose

CRISPR vector

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pRnc-gGFP129

Plasmid

#189537

Purpose

Expresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-129-GFP

Depositor

Marc Ostermeier

Article

Hauk et al CRISPR J. 2022 Dec 9. doi: 10.1089/crisp

Inserts

RNase III
gUTR-129-GFP

Expression

Bacterial

Mutation

N/A

Promoter

J23119 and pBAD

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pGEM-T-Easy-HySp5-557

Plasmid

#193000

Purpose

Plasmid to generate an ISH probe for Hydra Sp5

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Sp5

Use

Cloning vector

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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ZfSp5a-377

Plasmid

#192997

Purpose

Plasmid that encodes for Zebrafish Sp5

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Zebrafish Sp5

Tags

HA

Expression

Mammalian

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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HySp5-227

Plasmid

#192996

Purpose

Plasmid that encodes for Hydra Sp5, lacking the SP box and DNA binding domain

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Sp5

Tags

HA

Expression

Mammalian

Mutation

no SP box and DNA binding domain

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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ZfSp5l1

Plasmid

#192999

Purpose

Plasmid that encodes for Zebrafish Sp5-like

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Zebrafish Sp5-like

Tags

HA

Expression

Mammalian

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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HySp5-337

Plasmid

#192994

Purpose

Plasmid that encodes for Hydra Sp5, lacking the DNA binding domain

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Sp5

Tags

HA

Expression

Mammalian

Mutation

no DNA binding domain

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pETM20-avi-9S-S2 [rpoA]

Plasmid

#176223

Purpose

Expression of TRX-AviTag-9S-S2 [rpoA]

Depositor

Ian Small

Article

Bernath-Levin et al Synth Biol (Oxf). 2021 Dec 23;7(1):ysab0

Insert

TRX-Avi-9S-S2 [rpoA]

Use

Synthetic Biology

Tags

TRX-His-AviTag

Promoter

T7

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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rtTA-sg-CMV51

Plasmid

#188484

Purpose

gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 51.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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ppwd1.473.646.W601A

Plasmid

#137660

Purpose

Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

Depositor

Tara Davis, Melissa Jurica

Article

Adams et al Biochem J. 2015 Jul 15;469(2):223-33. do

Insert

ppwd1 (PPWD1 Human)

Tags

(His)6-thrombin

Expression

Bacterial

Mutation

473-646-W601A

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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KBB439

Plasmid

#185093

Purpose

NMA111-GFP wild type under control of GAL1 promoter (complements nma111 deletion)

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Tags

GFP

Expression

Yeast

Mutation

GAL1::NMA111-GFP

Promoter

GAL1

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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KBB462

Plasmid

#185095

Purpose

NMA111-GFP with NLS1 and NLS2 mutated under control of GAL1 promoter

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Tags

GFP

Expression

Yeast

Mutation

GAL1::nma111Dnls1Dnls2-GFP

Promoter

GAL1

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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KBB394

Plasmid

#185086

Purpose

NMA111 with both nuclear localization signals mutated to alanines fused to GFP (mutagenesis of KBB280)

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Tags

GFP

Expression

Yeast

Mutation

Nma111 KKR 9-11 AAA; KRK 28-30 AAA

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP.N3-myc-SLC35A2

Plasmid

#186285

Purpose

transient expression of N-terminal myc tagged SLC35A2 isoform c/UGT1 in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Li et al Glycobiology. 2019 Jun 1;29(6):490-503.

Insert

SLC35A2 (SLC35A2 Human)

Tags

MYC

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP humanAPOBEC1 L173A/G227A

Plasmid

#167171

Purpose

Expresses human APOBEC1 L173A/G227A in mammalian cells

Depositor

Silvestro Conticello

Article

Chieca et al bioRxiv 2021

Insert

humanAPOBEC1 (APOBEC1 Human)

Expression

Mammalian

Mutation

changed Leucine 173 to Alanine; changed Glycine 2…

Promoter

CMV promoter

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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Soat2_Luc

Plasmid

#177623

Purpose

Cloned human Soat2 promoter containing exon1 and 2 coupled to luciferase

Depositor

Thomas Burris

Article

Griffett et al ACS Chem Biol. 2022 Apr 13. doi: 10.1021

Insert

human Soat2

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pBM13g-sd4U

Plasmid

#179689

Purpose

Bacterial expression of base editor

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

[sd4U] UGI-TALE3-DddA-G1397-N (S1330I + A1341V + N1342S + E1370K + T1380I)

Expression

Bacterial

Mutation

S1330I A1341V N1342S E1370K T1380I

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pJH2108

Plasmid

#179252

Purpose

Punc-4::TWK-18(gf)-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of TWK-18-wCherry

Depositor

Mei Zhen

Article

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

TWK-18 (twk-18 Nematode)

Tags

wCherry

Expression

Worm

Mutation

M280I

Promoter

Punc-4

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pJH2106

Plasmid

#179250

Purpose

Pnmr-1::TWK-18(gf)-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of TWK-18-wCherry

Depositor

Mei Zhen

Article

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

TWK-18 (twk-18 Nematode)

Tags

wCherry

Expression

Worm

Promoter

Pnmr-1

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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