Addgene

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pUlaR

Plasmid

#216327

Purpose

T7 promoter driving expression of ulaR

Depositor

Mark Styczynski

Article

Piorino et al ACS Synth Biol. 2022 Nov 18;11(11):3592-

Insert

L-Ascorbate-6-P-responsive transciptional repressor

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pBad_UlaABC

Plasmid

#216328

Purpose

Arabinose-inducible PBad promoter driving expression of ulaA, ulaB, and ulaC

Depositor

Mark Styczynski

Article

Piorino et al ACS Synth Biol. 2022 Nov 18;11(11):3592-

Insert

UlaABC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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INT_Cargo_PgyrA_katG_dadA6

Plasmid

#212142

Purpose

Cargo plasmid for integrating PgyrA expression cassette of catalase-peroxidase and D-amino acid dehydrogenase into the genome. Plasmid can be removed by incubating cells at 37 C.

Depositor

Kang Zhou

Insert

catalase-peroxidase, D-amino acid dehydrogenase (katG katG: E. coli, dadA6: Cupriavidus necator)

Use

Tags

Expression

Bacterial

Mutation

Promoter

PgyrA

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAW188

Plasmid

#113178

Purpose

E. coli expression vector for untagged E. coli Cas1 R131A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Use

Tags

Expression

Bacterial

Mutation

R131A

Promoter

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAW167

Plasmid

#113187

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 R40E

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Use

Tags

Expression

Bacterial

Mutation

R40E

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW156

Plasmid

#113185

Purpose

E. coli expression vector for untagged E. coli Cas1 K259E + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Use

Tags

Expression

Bacterial

Mutation

K259E

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW186

Plasmid

#113184

Purpose

E. coli expression vector for untagged E. coli Cas1 R163A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Use

Tags

Expression

Bacterial

Mutation

R163A

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW151

Plasmid

#113183

Purpose

E. coli expression vector for untagged E. coli Cas1 R146A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Use

Tags

Expression

Bacterial

Mutation

R146A

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW150

Plasmid

#113182

Purpose

E. coli expression vector for untagged E. coli Cas1 S143A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Use

Tags

Expression

Bacterial

Mutation

S143A

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW174

Plasmid

#113181

Purpose

E. coli expression vector for untagged E. coli Cas1 R138A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Use

Tags

Expression

Bacterial

Mutation

R138A

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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