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Addgene

We narrowed to 52,749 results for: plasmid

Showing: 1601 - 1650 of 52749 results
  1. pLV-SFFV-mCherry-WPRE-UbC-Emerald

    Plasmid
    #211797
    Purpose
    Lentiviral vector plasmid expressing mCherry under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Inserts
    mCherry
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. PTNA-c200

    Plasmid
    #205114
    Purpose
    PTN protein expression
    Depositor
    Insert
    PTN (PTN Human)
    Use
    Tags
    AviTag-6His and PTPRSsp
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. Kv1.1/RBG4

    Plasmid
    #196943
    Purpose
    Plasmid expressing an antigen targeting the Kv1.1 potassium channel subunit
    Depositor
    Insert
    Kv1.1 (Kcna1 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. KChIP2/RBG4; 9QM3/RBG4

    Plasmid
    #196944
    Purpose
    Plasmid expressing an antigen targeting the KChIP2b potassium channel subunit
    Depositor
    Insert
    KChIP2 (Kcnip2 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. BAF53b + GFP/p-Actin-IRES

    Plasmid
    #197297
    Purpose
    Plasmid expressing an antigen targeting BAF53b
    Depositor
    Insert
    BAF53b (Actl6b Mouse)
    Use
    Tags
    GFP (GFP observed)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 1, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. pCEV-G2-Km ymTurquoise2

    Plasmid
    #193958
    Purpose
    TEF1 promoter controlled expression plasmid with G418 resistance for expressing ymTurquoise2 in budding yeast
    Depositor
    Insert
    ymTurquoise2
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. Kir2.1-HA/GW1

    Plasmid
    #197342
    Purpose
    Plasmid expressing an antigen targeting the Kir2.1 potassium channel subunit
    Depositor
    Insert
    Kir2.1 (Kcnj2 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 24, 2023
    Availability
    Academic Institutions and Nonprofits only
  8. Sur2A flag/pCDNA3

    Plasmid
    #197295
    Purpose
    Plasmid expressing an antigen targeting Sur2A
    Depositor
    Insert
    Sur2A (Abcc9 Mouse)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
  9. mDlx-Aion-citrine

    Plasmid
    #192572
    Purpose
    AAV-vector for optogenetic long-term silencing of GABAergic neurons
    Depositor
    Insert
    Aion
    Use
    AAV
    Tags
    Citrine
    Expression
    Mammalian
    Mutation
    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…
    Promoter
    minimal Dlx
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  10. CaMKII-somBiPOLES-ChroME2s-mCerulean

    Plasmid
    #192582
    Purpose
    AAV-vector for bidirectional optogenetic manipulation of excitatory neurons with blue shifted excitation spectrum
    Depositor
    Insert
    somBiPOLES
    Use
    AAV
    Tags
    soma-targeting motif from Kv2.1 channel
    Expression
    Mammalian
    Mutation
    Promoter
    CaMKIIa(0.4)
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  11. CaMKII-somBiPOLES-ChroME2f-mCerulean

    Plasmid
    #192581
    Purpose
    AAV-vector for bidirectional optogenetic manipulation of excitatory neurons with blue shifted excitation spectrum
    Depositor
    Insert
    somBiPOLES
    Use
    AAV
    Tags
    soma-targeting motif from Kv2.1 channel
    Expression
    Mammalian
    Mutation
    Promoter
    CaMKIIa(0.4)
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  12. hSyn-Aion-mKate2

    Plasmid
    #192574
    Purpose
    AAV-vector for optogenetic long-term silencing of neurons
    Depositor
    Insert
    Aion
    Use
    AAV
    Tags
    mKate2
    Expression
    Mammalian
    Mutation
    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…
    Promoter
    human synapsin
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  13. pSuper-si-CS

    Plasmid
    #187854
    Purpose
    a human siRNA-expressing plasmid targeting CS
    Depositor
    Insert
    si-CS (CS Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
  14. pSuper-si-MAPRE1

    Plasmid
    #187855
    Purpose
    a human siRNA-expressing plasmid targeting MAPRE1
    Depositor
    Insert
    si-MAPRE1 (MAPRE1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
  15. pmr101A-cPr-mNeonGreen (ErmR)

    Plasmid
    #177208
    Purpose
    Plasmid with p15A origin for constitutive mNeonGreen expression in E. coli
    Depositor
    Insert
    mNeonGreen
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Yeast codon optimized
    Promoter
    Synthetic constitutive promoter
    Available since
    March 16, 2022
    Availability
    Academic Institutions and Nonprofits only
  16. pLV V5-Senp2

    Plasmid
    #175118
    Purpose
    Lentiviral expression of V5-tagged mouse Senp2
    Depositor
    Insert
    Senp2 (Senp2 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  17. RDXB-c004

    Plasmid
    #175479
    Purpose
    Protein expression in bacterial cells. FERM domain, M1-A321.
    Depositor
    Insert
    RDX (RDX Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  18. pSBbi-RB-PE2

    Plasmid
    #173902
    Purpose
    SB-transposon with constitutive expression of SpCas9 PE2
    Depositor
    Insert
    PE2
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
  19. pNSU299_MET16

    Plasmid
    #166091
    Purpose
    Plasmid for constituive spCas9 and tet-inducible MET16 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Insert
    MET16 (MET16 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    May 14, 2021
    Availability
    Academic Institutions and Nonprofits only
  20. HCP1

    Plasmid
    #166103
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination.
    Depositor
    Insert
    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
  21. pNSU299_CPR1_1

    Plasmid
    #166074
    Purpose
    Plasmid for constituive spCas9 and tet-inducible CPR1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Insert
    CPR1 (CPR1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
  22. pNSU299_RRT8

    Plasmid
    #166080
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RRT8 for double stranded break formation in yeast.
    Depositor
    Insert
    Promoter of RRT8 (RRT8 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
  23. pNSU299_CPR1_2

    Plasmid
    #166071
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of an sgRNA targeting an intergenic site near CPR1 for double stranded break formation in yeast.
    Depositor
    Insert
    Intergenic region near CPR1
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
  24. pNSU299_GAL7

    Plasmid
    #166089
    Purpose
    Plasmid for constituive spCas9 and tet-inducible GAL7 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Insert
    GAL7 (GAL7 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
  25. pNSU299_GLK1

    Plasmid
    #166090
    Purpose
    Plasmid for constituive spCas9 and tet-inducible GLK1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Insert
    GLK1 (GLK1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  26. pNSU299_LHS1

    Plasmid
    #166092
    Purpose
    Plasmid for constituive spCas9 and tet-inducible LHS1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Insert
    LHS1 (LHS1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  27. pNSU299_RPL22A

    Plasmid
    #166079
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RPL22A for double stranded break formation in yeast.
    Depositor
    Insert
    Promoter of RPL22A (RPL22A Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  28. pNSU299_YBL055C

    Plasmid
    #166077
    Purpose
    Plasmid for constituive spCas9 and tet-inducible YBL055C targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Insert
    YBL055C (near PTC3) (YBL055C Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
  29. pNSU299_YNR071C

    Plasmid
    #166081
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of YNR071C for double stranded break formation in yeast.
    Depositor
    Insert
    Promoter of YNR071C (YNR071C Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
  30. pNSU299_SLA1

    Plasmid
    #166075
    Purpose
    Plasmid for constituive spCas9 and tet-inducible SLA1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Insert
    SLA1 (SLA1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
  31. pNSU299_PDR12

    Plasmid
    #166093
    Purpose
    Plasmid for constituive spCas9 and tet-inducible PDR12 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Insert
    PDR12 (PDR12 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
  32. pNSU299_TDH2

    Plasmid
    #166082
    Purpose
    Plasmid for constituive spCas9 and tet-inducible TDH2 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Insert
    TDH2 (TDH2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
  33. pNSU299_LYS9

    Plasmid
    #166085
    Purpose
    Plasmid for constituive spCas9 and tet-inducible LYS9 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Insert
    LYS9 (LYS9 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
  34. pANY4-pR18/pL18-ccdB

    Plasmid
    #164899
    Purpose
    Two valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pR18/pL18-ccdB.
    Depositor
    Insert
    ccdB cassette
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
  35. pLL1271

    Plasmid
    #129282
    Purpose
    Expression plasmid with pLL1270 as backbone and alpha-Xylp(Ga0256695_ 1211)
    Depositor
    Insert
    alpha-Xylp_1211 (Ga0256695_ 1211)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Clostridium thermocellum DSM1313 Cellobiose Phosp…
    Available since
    March 19, 2020
    Availability
    Academic Institutions and Nonprofits only
  36. pZSCAN18-donor

    Plasmid
    #113821
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZSCAN18 with GFP
    Depositor
    Insert
    ZSCAN18 homology arms flanking EGFP-IRES-Neo cassette (ZSCAN18 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 11, 2019
    Availability
    Academic Institutions and Nonprofits only
  37. FUW-hTie1-ECD-FLAG

    Plasmid
    #119235
    Purpose
    Plasmid encodes FLAG-tagged extracellular domain of human Tie1 gene cloned in lentiviral vector.
    Depositor
    Insert
    TIE1-ECD-FLAG (TIE1 Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mutation
    Contains extracellular domain (ECD) only
    Promoter
    P hUbC
    Available since
    Dec. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
  38. pZNF561-donor

    Plasmid
    #113801
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF561 with GFP
    Depositor
    Insert
    ZNF561 homology arms flanking EGFP-IRES-Neo cassette (ZNF561 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
  39. pZNF700-donor

    Plasmid
    #112385
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF700 with GFP
    Depositor
    Insert
    ZNF700 homology arms flanking EGFP-IRES-Neo cassette (ZNF700 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
  40. pZNF169-donor

    Plasmid
    #112376
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF169 with GFP
    Depositor
    Insert
    ZNF169 homology arms flanking EGFP-IRES-Neo cassette (ZNF169 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  41. pZFP1-donor

    Plasmid
    #112374
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZFP1 with GFP
    Depositor
    Insert
    ZFP1 homology arms flanking EGFP-IRES-Neo cassette (ZFP1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  42. pZNF227-donor

    Plasmid
    #112368
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF227 with GFP
    Depositor
    Insert
    ZNF227 homology arms flanking EGFP-IRES-Neo cassette (ZNF227 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  43. pOTX1-donor

    Plasmid
    #112364
    Purpose
    CRISPR donor plasmid to tag human transcription factor OTX1 with GFP
    Depositor
    Insert
    OTX1 homology arms flanking EGFP-IRES-Neo cassette (OTX1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  44. pZNF175-donor

    Plasmid
    #112347
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF175 with GFP
    Depositor
    Insert
    ZNF175 homology arms flanking EGFP-IRES-Neo cassette (ZNF175 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  45. pZNF354C-donor

    Plasmid
    #112339
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF354C with GFP
    Depositor
    Insert
    ZNF354C homology arms flanking EGFP-IRES-Neo cassette (ZNF354C Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
  46. pZNF347-donor

    Plasmid
    #112326
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF347 with GFP
    Depositor
    Insert
    ZNF347 homology arms flanking EGFP-IRES-Neo cassette (ZNF347 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
  47. STK10

    Plasmid
    #74659
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    STK10 (STK10 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 10, 2016
    Availability
    Academic Institutions and Nonprofits only
  48. pRP-iSL(MCS1+2)

    Plasmid
    #69245
    Purpose
    Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a random RRNA spacer locus in T. brucei.
    Depositor
    Type
    Empty backbone
    Use
    Expression in trypanosoma brucei
    Tags
    Expression
    Mutation
    Promoter
    RRNA promoter
    Available since
    Oct. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
  49. AAV-sCAG-eGFP-ACAP1

    Plasmid
    #203800
    Purpose
    AAV vector plasmid expressing human ACAP1 fused to eGFP under a short variant of the CAG (sCAG) promoter
    Insert
    ACAP1 (ACAP1 Human)
    Use
    AAV
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 1601 - 1650 of 52749 results