We narrowed to 2,718 results for: fas

1. VCP(wt)-EGFP

   Plasmid

   #23971

   Depositor

   Nico Dantuma

   Article

   Tresse et al Autophagy. 2010 Feb 22. 6(2):.

   Insert

   Valosin containing protein (VCP Human)
   

   Tags

   6xHis, EGFP, and HA

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Feb. 26, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-EGFP-RhoA-T19N

   Plasmid

   #12967

   Depositor

   Gary Bokoch

   Article

   Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

   Insert

   RhoA DN (RHOA Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   T19N Dominant negative

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-EGFP-Cdc42-T17N

   Plasmid

   #12976

   Depositor

   Gary Bokoch

   Article

   Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

   Insert

   Cdc42 DN (CDC42 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   T17N Dominant negative

   Promoter

   CMV

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-EGFP-Cdc42-Q61L

   Plasmid

   #12986

   Depositor

   Gary Bokoch

   Article

   Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

   Insert

   Cdc42 constitutively active (CDC42 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Q61L Constitutively active

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. lenti U6-sgRNA/EF1a-mCherry

   Plasmid

   #114199

   Purpose

   Lentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoter

   Depositor

   Jeremy Day

   Article

   Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

   Insert

   sgRNA scaffold
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-EGFP-Rac1-Q61L

   Plasmid

   #12981

   Depositor

   Gary Bokoch

   Article

   Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

   Insert

   Rac1 constitutively active (RAC1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Q61L Constitutively active

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. lenti EF1a-FLAG-dCas9-VPR

   Plasmid

   #114195

   Purpose

   Lentivirus compatible dCas9-VPR construct driven by the EF1a promoter

   Depositor

   Jeremy Day

   Article

   Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

   Insert

   dCas9-VPR
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-EGFP-Rac1-T17N

   Plasmid

   #12982

   Depositor

   Gary Bokoch

   Article

   Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

   Insert

   Rac1 DN (RAC1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   T17N Dominant negative

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA

   Plasmid

   #61592

   Purpose

   A human codon-optimized Cas9 from Staphylococcus aureus (SaCas9).

   Depositor

   Feng Zhang

   Article

   Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

   Has Service

   AAV6 and AAV8

   Insert

   hSaCas9
   

   Use

   AAV and CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Available Since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. VCP(R155H)-EGFP

    Plasmid

    #23972

    Depositor

    Nico Dantuma

    Article

    Tresse et al Autophagy. 2010 Feb 22. 6(2):.

    Insert

    Valosin containing protein (VCP Human)
    

    Tags

    6xHis, EGFP, and HA

    Expression

    Mammalian

    Mutation

    R155H

    Available Since

    Feb. 26, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

    Plasmid

    #42229

    Purpose

    This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. lenti SYN-dCas9-KRAB-MeCP2

    Plasmid

    #155365

    Purpose

    Expresses dCas9-KRAB-MeCP2 fusion driven by human SYN promoter

    Depositor

    Jeremy Day

    Article

    Duke et al Front Genome Ed. 2020 Sep 15;2:9. doi: 1

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR and Lentiviral

    Tags

    3x FLAG and 7x His

    Expression

    Mammalian

    Promoter

    SYN

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA

    Plasmid

    #61594

    Purpose

    A catalytically inactive SaCas9 (dSaCas9) with the D10A and N580A mutations.

    Depositor

    Feng Zhang

    Article

    Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

    Insert

    hSaCas9
    

    Use

    AAV and CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    D10A, N580A

    Available Since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. lenti CAG-FLAG-dCas9-VPR

    Plasmid

    #114197

    Purpose

    Lentivirus compatible dCas9-VPR construct driven by the CAG promoter

    Depositor

    Jeremy Day

    Article

    Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

    Insert

    dCas9-VPR
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. VCP(DK0)-EGFP

    Plasmid

    #23974

    Depositor

    Nico Dantuma

    Article

    Tresse et al Autophagy. 2010 Feb 22. 6(2):.

    Insert

    Valosin containing protein (VCP Human)
    

    Tags

    6xHis, EGFP, and HA

    Expression

    Mammalian

    Mutation

    E305QE578Q

    Available Since

    March 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-EGFP-Cdc42-wt

    Plasmid

    #12975

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    Cdc42 (CDC42 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FZ-NbPDS

    Plasmid

    #184268

    Purpose

    pAIDE containing a full-length CPSMV RNA2 cDNA modified to accomodate an NbPDS fragment.

    Depositor

    Feng Qu

    Article

    Zaulda et al Plant Methods. 2022 Oct 28;18(1):116. do

    Insert

    CPSMV RNA2 cDNA, modified to permit foreign gene insertion between MP and L-CP
    

    Expression

    Plant

    Mutation

    The protease processing site between MP and L-CP …

    Promoter

    CaMV 35S with duplicated enhancer

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

    Plasmid

    #42337

    Purpose

    Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. VCP(A232E)-EGFP

    Plasmid

    #23973

    Depositor

    Nico Dantuma

    Article

    Tresse et al Autophagy. 2010 Feb 22. 6(2):.

    Insert

    Valosin containing protein (VCP Human)
    

    Tags

    6xHis, EGFP, and HA

    Expression

    Mammalian

    Mutation

    A232E

    Available Since

    Feb. 26, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJC-repo1.9kb-TALE4-VP64-P10

    Plasmid

    #104612

    Purpose

    Expresses TALE4 under the control of a 1.9kb repo enhancer element

    Depositor

    Tudor Fulga

    Article

    Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

    Insert

    1.9kb repo enhancer element (repo Fly)
    

    Use

    TALEN

    Expression

    Insect

    Promoter

    hsp70

    Available Since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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