Addgene

We narrowed to 2,857 results for: human cdnas

Showing: 641 - 660 of 2857 results

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pcDNA5-FRT-GFP-mCherry-3pGW

Plasmid

#53965

Purpose

Bidirectional construct in the pcDNA5/FRT backbone with GFP and mCherry driven by the same CMV promoter along with a Gateway cloning site downstream mCherry

Depositor

Saeed Tavazoie

Article

Oikonomou et al Cell Rep. 2014 Apr 10;7(1):281-92. doi:

Inserts

GFP
mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TetO-U1-70K-fSNAPc

Plasmid

#106987

Purpose

open reading frame of U1-70K inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

Depositor

Melissa Moore

Article

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

U1-70K (SNRNP70 Human)

Use

Tags

fSNAP

Expression

Mutation

Promoter

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-hygro-eGFP-HEC1-8A/S55D

Plasmid

#114032

Purpose

Inducible expression of eGFP-tagged HEC1-8A/S55D in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-HEC1-8A/S55D (NDC80) (NDC80 Human)

Use

Tags

Expression

Mammalian

Mutation

Hec1 - 8A/S55D

Promoter

Available since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-WT

Plasmid

#215898

Purpose

Expression of TNRC6A-CIM1 domain

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Use

Tags

myc-GFP

Expression

Mammalian

Mutation

contain aa1224-1528

Promoter

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-GFP-TNRC6A-SD-CIM2-WT

Plasmid

#215899

Purpose

Expression of TNRC6A-CIM2 domain

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Use

Tags

myc-GFP

Expression

Mammalian

Mutation

contain aa1529-1709

Promoter

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-puro-eGFP-HEC1-d80

Plasmid

#114025

Purpose

Inducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-d80-HEC1 (NDC80) (NDC80 Human)

Use

Tags

Expression

Mammalian

Mutation

Hec1 - 1 to 80 deleted

Promoter

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-hygro-eGFP-HEC1-d80

Plasmid

#114030

Purpose

Inducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-d80-HEC1 (NDC80) (NDC80 Human)

Use

Tags

Expression

Mammalian

Mutation

Hec1 - 1 to 80 deleted

Promoter

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-hygro-eGFP-HEC1-8A/S15D

Plasmid

#114031

Purpose

Inducible expression of eGFP-tagged HEC1-8A/S15D in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-HEC1-8A/S15D (NDC80) (NDC80 Human)

Use

Tags

Expression

Mammalian

Mutation

Hec1 - 8A/S15D

Promoter

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-NL63-D14

Plasmid

#172666

Purpose

encodes NL63 spike protein, for pseudovirus production

Depositor

David Nemazee

Article

Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

Insert

HCNV63gp2 (HCNV63gp2 human coronavirus NL63)

Use

Lentiviral and Retroviral

Tags

NA

Expression

Mammalian

Mutation

c-terminal deletion of 14aa

Promoter

CMV

Available since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TopBP1-mCer

Plasmid

#211414

Purpose

mammalian expression of human TopBP1 C-terminally fused to mCerulean

Depositor

Helmut Pospiech

Insert

TopBP1 (TOPBP1 Human)

Use

Tags

mCerulean

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TopBP1-mVen

Plasmid

#211415

Purpose

mammalian expression of human TopBP1 C-terminally fused to mVenus

Depositor

Helmut Pospiech

Insert

TopBP1 (TOPBP1 Human)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RecQL4-mVen

Plasmid

#211403

Purpose

mammalian expression of human RecQL4 C-terminally fused to mVenus

Depositor

Helmut Pospiech

Insert

RecQL4 (RECQL4 Human)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pY120 (pcDNA3.1-huFbCpf1)

Plasmid

#92296

Purpose

Expression of humanized FbCpf1

Depositor

Feng Zhang

Insert

huFbCpf1

Use

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2017

Availability

Academic Institutions and Nonprofits only

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pY126 (pcDNA3.1-huBoCpf1)

Plasmid

#92302

Purpose

Expression of humanized BoCpf1

Depositor

Feng Zhang

Insert

huBoCpf1

Use

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pY123 (pcDNA3.1-huCMaCpf1)

Plasmid

#92299

Purpose

Expression of humanized CMaCpf1

Depositor

Feng Zhang

Insert

huCMaCpf1

Use

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pY114 (pcDNA3.1-huPgCpf1)

Plasmid

#92273

Purpose

Expression of humanized PgCpf1

Depositor

Feng Zhang

Insert

huPgCpf1

Use

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pY122 (pcDNA3.1-huCPbCpf1)

Plasmid

#92298

Purpose

Expression of humanized CPbCpf1

Depositor

Feng Zhang

Insert

huCPbCpf1

Use

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RecQL4-mCer

Plasmid

#211402

Purpose

mammalian expression of human RecQL4 C-terminally fused to mCerulean

Depositor

Helmut Pospiech

Insert

RecQL4 (RECQL4 Human)

Use

Tags

mCerulean

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(+)-CMV-bArrestin2-TEV

Plasmid

#107245

Purpose

Vector for preparation of stable cell lines expressing Barrestin2-TEV fusion protein in lieu of the HTLA cell line.

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

ARRB2

Use

Tags

TEV(NIA), tobacco etch virus NIA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3−3xFLAG−AGO2(D669A)

Plasmid

#220461

Purpose

Express catalytically-dead mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

Human AGO2 (AGO2 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Aspartate 669 to alanine

Promoter

CMV

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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