We narrowed to 57,330 results for: plasmid
-
Plasmid#85581PurposeReplicating base vector for constructing cpf1/CRISPR editing plasmidsDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by
-
pMXs-Oct3/4
Plasmid#13366PurposeRetroviral expression of mouse Oct3/4DepositorInsertPOU domain, class 5, transcription factor 1 (Pou5f1 Mouse)
UseRetroviralExpressionMammalianMutationNoneAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFD5_w
Plasmid#112645PurposeUbiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.DepositorInsertU6:3-tRNA-sgRNA expression cassette
ExpressionInsectAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMXs-hKLF4
Plasmid#17219PurposeRetroviral expression of human KLF4DepositorAvailable sinceFeb. 8, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lentiGuide-Hygro-mTagBFP2
Plasmid#99374PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A mTagBFP2 from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
Hygro-P2A-mTagBFP2
UseCRISPR and LentiviralExpressionMammalianPromoterEF-1a and hU6Available sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-GFAP-hM4D(Gi)-mCherry
Plasmid#50479PurposeGi-coupled hM4D DREADD fused with mCherry under the control of GFAP promoterDepositorHas serviceAAV5InserthM4D(Gi)-mCherry
UseAAVTagsmCherryMutationSee supplemental documents for DREADD mutationsPromoterGFAPAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Tol2-elavl3-GCaMP6s
Plasmid#59531PurposeExpressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backboneDepositorInsertsAvailable sinceSept. 12, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pdCas9
Plasmid#46569PurposeExpresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.DepositorPublicationInsertstracrRNA
dcas9
CRISPR array
UseCRISPRExpressionBacterialMutationD10A, H840AAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lenti sgRNA(MS2)_zeo backbone
Plasmid#61427Purpose3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorHas serviceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterU6 and EF1AAvailable sinceDec. 15, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCBC-DT1T2
Plasmid#50590PurposeCRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sitesDepositorInsertT1, U626t plus, U629p, T2
UseCRISPR; Pcr templateExpressionPlantAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MSCV Puro
Plasmid#68469PurposeRetroviral plasmid with multiple cloning site and puromycin resistanceDepositorInsertPuromycin Resistance
UseRetroviralExpressionMammalianPromoterpGKAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUCmini-iCAP-PHP.B
Plasmid#103002Purposenon-standard AAV2 rep-AAV-PHP.B cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.B VP1 gene
UseAAVExpressionMammalianPromoterp41Available sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUCM-AAVS1-TO-hNGN2
Plasmid#105840PurposeDonor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuronDepositorInsertsUseCRISPR and TALENPromoterCAG, EF-1alpha, and TRE3GAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET51b(+)_EGFP_NbALFA
Plasmid#136626PurposePlasmid encoding the bacterial expression of NbALFA for ALFA-tag as an EGFP-fusionDepositorInsertEGFP-NbALFA
TagsHisx10 and StrepExpressionBacterialPromoterT7Available sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ERmoxGFP
Plasmid#68072Purposemammalian expression of ER localized moxGFPDepositorInsertmoxGFP
TagsKDEL ER retention sequence and bovine prolactin s…ExpressionMammalianPromoterCMVAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLJM1-FIRE-pHLy
Plasmid#170775PurposeRatiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cellsDepositorInsertLAMP1 (LAMP1 Human)
UseLentiviralTagsmCherry and mTFP1ExpressionMammalianMutation84 nucleotide Signal Sequence switched to the beg…PromoterCMVAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pECE-HA-AMPKalpha2 wt
Plasmid#31654DepositorInsertprotein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2 Human)
TagsHA (YDVPDYASL)ExpressionMammalianAvailable sinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
phU6-gRNA
Plasmid#53188PurposeExpresses the S. pyogenes sgRNA from the human U6 promoterDepositorHas serviceCloning Grade DNATypeEmpty backboneUseCRISPRExpressionMammalianPromoterhuman U6Available sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB-TRE-EGFP-EF1a-rtTA
Plasmid#104454PurposePiggyBac plasmid expressing EGFP under the control of a doxycycline-inducible promotorDepositorInsertsenhanced green fluorescent protein
reverse tetracycline-controlled transactivator 3
UsePiggybacExpressionMammalianAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ230 (LbCpf1)
Plasmid#86210PurposeLbCpf1 Gateway entry plasmidDepositorInsertLbCpf1
UseCRISPR; Gateway compatible lbcpf1 entry cloneTags5' and 3' NLSExpressionPlantAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by