We narrowed to 7,351 results for: ski

1. pET28-his6-HA3-CLIP

   Plasmid

   #188942

   Purpose

   T7 expression vector for making his6- and HA3-tagged CLIP protein

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   his6-HA3-CLIP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAH6

   Plasmid

   #41830

   Purpose

   Used as a PCR template for tagging yeast proteins with the SNAP tag by homologous recombination and selection for ClonNAT resistance

   Depositor

   Melissa Moore

   Article

   Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

   Type

   Empty backbone

   Use

   Pcr template

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   T7,TEF

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVG48

   Plasmid

   #164706

   Purpose

   rtTA under the control of PGK1 promoter

   Depositor

   Sahand Jamal Rahi

   Article

   Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

   Insert

   PGK1pr - rtTA - CYC1t
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Vh-HA-DHFR

   Plasmid

   #20217

   Depositor

   Carolyn Bertozzi

   Article

   Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

   Insert

   DHFR (folA E. coli)
   

   Use

   Tags

   HA and Vh

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

5. pAAH2

   Plasmid

   #41826

   Purpose

   Used as a PCR template for tagging yeast proteins with the DHFR tag by homologous recombination and selection for hygromycin resistance.

   Depositor

   Melissa Moore

   Article

   Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

   Type

   Empty backbone

   Use

   Pcr template

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   T7, TEF

   Available since

   Oct. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPtac

   Plasmid

   #166937

   Purpose

   Ptac single-level gene expression controller (SLC)

   Depositor

   Thomas Gorochowski

   Article

   Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

   Insert

   Genetic circuit expressing GFP- PTAC
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PTAC

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pE-01

   Plasmid

   #166930

   Purpose

   Stop codon part (E)

   Depositor

   Thomas Gorochowski

   Article

   Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

   Insert

   STOP CODON TAA and spacer - BLOCK E- Flanked by BsaI sites
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pD-01

   Plasmid

   #166929

   Purpose

   TrrnB and L3S2P21 terminator part (D)

   Depositor

   Thomas Gorochowski

   Article

   Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

   Insert

   DOUBLE TERMINATOR ( TrrnB - L3S2P21) _BLOCK D - Flanked by BsaI sites
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pB-01

   Plasmid

   #166924

   Purpose

   Ptac promoter part (B)

   Depositor

   Thomas Gorochowski

   Article

   Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

   Insert

   PTAC promoter - Block B- Flanked by BsaI restriction sites.
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pA-01

    Plasmid

    #166923

    Purpose

    LacI expression cassette (A)

    Depositor

    Thomas Gorochowski

    Article

    Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

    Insert

    LacI expression cassette - Block A- Flanked by BsaI sites
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAH1

    Plasmid

    #41825

    Purpose

    Used as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for hygromycin resistance.

    Depositor

    Melissa Moore

    Article

    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

    Type

    Empty backbone

    Use

    Pcr template

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    T7, TEF

    Available since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. U6-U57C

    Plasmid

    #169928

    Purpose

    WT U6 with a U57C mutation

    Depositor

    Aaron Hoskins

    Article

    van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

    Insert

    U6 U57C
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SG-3'REL

    Plasmid

    #27257

    Depositor

    Thomas Gilmore

    Article

    Starczynowski et al Oncogene. 2003 Oct 9. 22(44):6928-36.

    Insert

    REL (REL Human)
    

    Use

    Tags

    GAL4

    Expression

    Mammalian

    Mutation

    Contains aa 278-587 of REL

    Promoter

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

14. Vh-HA-3xDHFR

    Plasmid

    #20219

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    3xDHFR (folA E. coli)
    

    Use

    Tags

    HA and Vh

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

15. wt Cus2p

    Plasmid

    #73046

    Purpose

    Wild-type S. cerevisiae Cus2p gene for recombinant expression and crystallization

    Depositor

    Aaron Hoskins

    Article

    Rodgers et al RNA. 2015 Dec 2.

    Insert

    CUS2p (CUS2 Budding Yeast)
    

    Use

    Tags

    6x His tag fused to TEV cleavage site

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    T7

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRS413 Prp5 WT

    Plasmid

    #87599

    Purpose

    Encodes Prp5 WT for expression in yeast

    Depositor

    Aaron Hoskins

    Article

    Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

    Insert

    PRP5 (PRP5 )
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Vh-HA-2xDHFR

    Plasmid

    #20218

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    2xDHFR (folA E. coli)
    

    Use

    Tags

    HA and Vh

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pUC57-Homology Arms-BRD4-HaloTag

    Plasmid

    #228575

    Purpose

    CRISPR donor plasmid to tag human BRD4 with Halo tag; for CRISPR-mediated gene insertion

    Depositor

    Marcin Imielinski

    Insert

    Homology Arms-BRD4-HaloTag (BRD4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX330-U6-BRD4-HaloTag-Guide-hspCas9

    Plasmid

    #228576

    Purpose

    Expression of Halo-tagged human BRD4 gRNA; CRISPR-mediated gene insertion

    Depositor

    Marcin Imielinski

    Insert

    BRD4 gRNA (BRD4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBetaActin-dCer3-C2-dCer3

    Plasmid

    #191328

    Purpose

    Expresses the fluorescently labeled membrane anchor C2 without the chemical dimerization domain FRB as a control for recruiting proteins to plasma membrane

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    dCer3-C2-dCer3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge