We narrowed to 10,632 results for: t7

1. pKP2085

   Plasmid

   #167606

   Purpose

   Express ColicinB-V5-TEV-T7-mature MBP

   Depositor

   Kathleen Postle

   Insert

   cba-malE translational fusion
   

   Expression

   Bacterial

   Mutation

   cba ORF fused to V5 tag, TEV protease site, T7 ta…

   Available Since

   July 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET29b-H6_Streptavidin_sfGFP

   Plasmid

   #124296

   Purpose

   His6-tagged Streptavidin GFP fusion protein

   Depositor

   Mark Arbing

   Insert

   His6 tag - TEV protease site - T7 tag - Streptavidin (15-159) - superfolder GFP
   

   Tags

   His6 and T7 tags

   Expression

   Bacterial

   Available Since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pH6-TEV-mCherry

   Plasmid

   #132430

   Purpose

   Expresses His6-tagged mCherry in E. coli using T7 promoter

   Depositor

   Mark Arbing

   Insert

   mCherry
   

   Tags

   N-terminal His6 tag with TEV protease site

   Expression

   Bacterial

   Mutation

   Codon optimized

   Promoter

   T7

   Available Since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Click editor (CE1) mRNA expression vector - T7-5'UTR-PCV2-nCas9-EcKlenow-3'UTR-polyA (CJT472)

   Plasmid

   #217808

   Purpose

   Expression vector for mRNA production of the click editor construct (CE1; PCV2-nCas9-EcKlenow)

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   5'UTR-PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(exo-)-BPNLS-3'UTR
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   T7

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYZ205

   Plasmid

   #104586

   Purpose

   Express SNAP-6His-CBD

   Depositor

   Yinhua Zhang, New England Biolabs

   Insert

   SNAP-6His-CBD
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 4, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. Rich-pT7GG

   Plasmid

   #108443

   Purpose

   This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pT7TS-rich. The vector is compatible for Golden-gate assembly

   Depositor

   Richard Bélanger

   Type

   Empty backbone

   Use

   Xenopus oocyte expression system

   Promoter

   T7

   Available Since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. p15TV-L

   Plasmid

   #26093

   Purpose

   SGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Tags

   6xHis and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   Sept. 6, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. p70a-T7RNAP

   Plasmid

   #105683

   Purpose

   Expresses T7 polymerase in cell-free TXTL.

   Depositor

   Chase Beisel

   Article

   Maxwell et al Methods. 2018 Jul 1;143:48-57. doi: 10.1

   Insert

   T7 polymerase
   

   Expression

   Bacterial

   Promoter

   OR2-OR1-Pr (bacteriophage Lambda with one mutatio…

   Available Since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. p2,0_3E5E_eGFP

   Plasmid

   #69359

   Purpose

   Encodes for Ebola Minigenome with eGFP reporter gene under control of a T7 RNA polymerase promoter

   Depositor

   Elke Mühlberger

   Article

   Schmidt et al J Infect Dis. 2011 Nov;204 Suppl 3:S861-

   Insert

   3E5E eGFP minigenome
   

   Use

   T7

   Promoter

   T7

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET30b_T7Sav

    Plasmid

    #138588

    Purpose

    T7-tagged streptavidin with native C-terminus controlled by PT7 promoter; KanR, pBR322 ori

    Depositor

    Markus Jeschek

    Article

    Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10

    Insert

    T7-tagged streptavidin variant with native C-terminus
    

    Use

    Synthetic Biology

    Tags

    T7-tag

    Expression

    Bacterial

    Promoter

    PT7

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET:GACT:GFP

    Plasmid

    #60734

    Purpose

    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACT' between -11 and -8.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJin-264

    Plasmid

    #125717

    Purpose

    ABA-inducible split T7 gRNA for GFP vector using RNAPN d5-19

    Depositor

    Bryan Dickinson

    Article

    Pu et al ACS Chem Biol. 2018 Feb 16;13(2):431-437

    Insert

    PT7-gRNA GFP expression, PYL-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-ABI
    

    Expression

    Mammalian

    Available Since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET:GACG:GFP

    Plasmid

    #60735

    Purpose

    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACG' between -11 and -8.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET:GCAT:GFP

    Plasmid

    #60736

    Purpose

    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GCAT' between -11 and -8.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET:ACAT:GFP

    Plasmid

    #60740

    Purpose

    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'ACAT' between -11 and -8.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET:CCCT:GFP

    Plasmid

    #60738

    Purpose

    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CCCT' between -11 and -8.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJin-239

    Plasmid

    #125716

    Purpose

    ABA-inducible split T7 gRNA for GFP vector using RNAPN N-29-1

    Depositor

    Bryan Dickinson

    Article

    Pu et al ACS Chem Biol. 2018 Feb 16;13(2):431-437

    Insert

    pT7-gRNA GFP expression, PYL-linker-T7 RNAPC, T7 RNAPN (N-29-1)-linker-ABI
    

    Expression

    Mammalian

    Available Since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZB260_pT7-STR-H3U3

    Plasmid

    #228506

    Purpose

    Bacterial Hybrid Reporter Plasmid, pT7-HIS3/URA3

    Depositor

    Timothy Whitehead

    Article

    Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

    Insert

    HIS3/URA3 operon
    

    Use

    Synthetic Biology

    Promoter

    T7

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET:CACT:GFP

    Plasmid

    #60737

    Purpose

    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CACT' between -11 and -8.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pETM6-G6-vioABE-4A6-vioC

    Plasmid

    #73439

    Purpose

    Deoxyviolacein pathway (vioABEC) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE) and 4A6 (vioC) for orthogonal flux redirection.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    PG6-vioABE-P4A6-vioC
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    PG6 and P4A6 (orthogonal T7-lac variants)

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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