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pRS416 Gal-RNQ1 1-153-YFP

Plasmid

#18690

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Rnq1 1-153

Tags

YFP

Expression

Yeast

Mutation

NonPrion domain of Rnq1

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pMPRAduo:minP:GFP

Plasmid

#193739

Purpose

MPRAduo ORF (minP:GFP) donor

Depositor

Ryan Tewhey

Article

Mouri et al Cell Genom. 2022 Dec 16;3(1):100234. doi

Insert

GFP

Expression

Mammalian

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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11K-cyto-->R GLUT1-GFP

Plasmid

#200096

Purpose

human GLUT1 with K-->R mutations for all 11 cytosolic lysines and a C-terminal GFP tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

GLUT1 (SLC2A1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

K-->R mutations of amino acids 6, 7, 225, 229,…

Promoter

CMV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2282

Plasmid

#190363

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::AmtR-ERF4(RD)-19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::AmtR-ERF4(RD)-19St

Expression

Plant

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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Affibody EGFR:1907.BirA*-pET301/NT-DEST

Plasmid

#174691

Purpose

Plasmid encoding for the bacterial expression of a bispecific coding for the anti-EGFR affibody ZEGFR:1907 fused to the mutated form of BirA enzyme, BirA*

Depositor

Jean Gariepy

Article

Alwash et al ACS Pharmacol Transl Sci. 2022 Feb 1;5(2

Insert

Anti-EGFR affibody ZEGFR:1907 fused to mutated form of BirA, BirA*

Tags

HIS tag

Expression

Bacterial

Mutation

R118G mutation in BirA

Promoter

T7 promoter

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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CoV-NSP3-c001

Plasmid

#173082

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

rep

Tags

mCerulean

Expression

Bacterial

Mutation

E1000-K1173, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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mILK-R255A/R349A-EFGP

Plasmid

#176899

Purpose

mouse ILK with R255A and R349A point mutations cloned into pEGFP-N1

Depositor

Sara Wickström

Article

Martin et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

Insert

Integrin-linked kinase (Ilk Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Arginine 255 to Alanine and Arginine 349 to Alani…

Promoter

CNV

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMHE:hTRAAK(Q258K)

Plasmid

#133077

Purpose

X. laevis expression vector. It will generate the human TRAAK channel

Depositor

Dan Minor

Article

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1), Q258K (Kcnk2 Mouse)

Mutation

Q258K

Promoter

T7

Available Since

Oct. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pSU313

Plasmid

#169695

Purpose

Template plasmid for generating C-terminal 1xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.

Depositor

Nara Figueroa

Article

Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

Insert

1xFLAG epitope tag adjacent to FRT-flanked cat (CamR) gene

Tags

1xFLAG tag

Expression

Bacterial

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pSU314

Plasmid

#169696

Purpose

Template plasmid for generating C-terminal hemagglutinin (1xHA) epitope tag fusions to bacterial genes by "Lambda red" recombineering.

Depositor

Nara Figueroa

Article

Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

Insert

1xHA epitope tag adjacent to FRT-flanked cat (CamR) gene

Tags

1xHA tag

Expression

Bacterial

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAS1_4xh7SKPylT_EF1_GFP*_Intein_DD

Plasmid

#162812

Purpose

STELLA plasmid for amber suppression of C-terminally labelled GFP

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

GFP(C-TAG)-Intein-DD

Use

Piggybac

Expression

Mammalian

Mutation

GFP(Opal247,Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pSec7-Halox2

Plasmid

#115425

Purpose

Express Sec7-Halox2 at the endogenous locus via pop-in pop-out mutagenesis

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

Sec7-Halox2

Tags

HaloTag

Expression

Yeast

Promoter

Endogenous

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-vps10-104

Plasmid

#115426

Purpose

Generate the vps10-104 allele via pop-in pop-out mutagenesis

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

vps10-104

Expression

Yeast

Mutation

domain 1 deletion

Promoter

Endogenous

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-Erg11-iGFPx3

Plasmid

#115422

Purpose

Express Erg11-iGFPx3 at the endogenous locus via pop-in pop-out mutagenesis

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

Erg11-iGFPx3

Tags

iGFP

Expression

Yeast

Promoter

Endogenous

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac204-pOst1-ESCargo

Plasmid

#115420

Purpose

Expresses a regulatable secretory cargo for yeast at the TRP1 locus

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

pOst1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Expression

Yeast

Promoter

TPI1

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pLMB684

Plasmid

#83779

Purpose

Xylose biosensor plasmid

Depositor

Philip Poole

Article

Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

Insert

upstream region of RL2720 (rbsC)

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMDC84-SEIPIN1

Plasmid

#96979

Purpose

Express GFP-tagged Arabidopsis SEIPIN1 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN1 (AT5G16460 Mustard Weed)

Tags

6xHis and GFP

Expression

Plant

Promoter

35S

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pMDC43-SEIPIN3

Plasmid

#96978

Purpose

Express GFP-tagged Arabidopsis SEIPIN3 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN3 (AT2G34380 Mustard Weed)

Tags

GFP

Expression

Plant

Promoter

35S

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pLMB687

Plasmid

#83781

Purpose

Sucrose biosensor plasmid

Depositor

Philip Poole

Article

Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

Insert

Region upstream pRL120556 containing all of pRL120555

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pYFP-ErbB2ΔC776

Plasmid

#66947

Purpose

Mammalian expression of rat ErbB2 ΔC776 tagged with YFP

Depositor

Mads Lerdrup, Bo van_Deurs

Article

Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E

Insert

ErbB2 (Erbb2 Rat)

Tags

6xHis, V5 Tag, and YFP

Expression

Mammalian

Mutation

Deletion of C776 (see depositor comment below on …

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only