We narrowed to 10,016 results for: ADA

1. AT2G33170 X049_pECIA14

   Plasmid

   #114901

   Purpose

   Prey vector AT2G33170 X049_pECIA14 should be used with bait vector AT2G33170 X049_pECIA2.

   Depositor

   Youssef Belkhadir

   Article

   Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

   Insert

   AT2G33170 (AT2G33170 Mustard Weed)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Metallothionein (Copper-Inducible)

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. AT1G74360 X038_pECIA14

   Plasmid

   #114880

   Purpose

   Prey vector AT1G74360 X038_pECIA14 should be used with bait vector AT1G74360 X038_pECIA2.

   Depositor

   Youssef Belkhadir

   Article

   Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

   Insert

   AT1G74360 (AT1G74360 Mustard Weed)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Metallothionein (Copper-Inducible)

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCG_batCoV RaTG13 N C-V5-tag

   Plasmid

   #179987

   Purpose

   Mammalian expression vector for RaTG13 N, V5-tagged. Sequence codon-optimized.

   Depositor

   Konstantin Sparrer

   Article

   Hayn et al Cell Rep. 2021 May 18;35(7):109126.

   Insert

   RaTG13 N
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   human codon-optimized

   Promoter

   Available Since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-GfABC1D-SaCas9-WPRE3-pA

   Plasmid

   #203540

   Purpose

   SaCas9 expression in astrocytes

   Depositor

   Ben Deverman

   Article

   Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

   Insert

   SaCas9
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-GfABC1D-NLS-GFP-WPRE-synpA-L1-R2

   Plasmid

   #203541

   Purpose

   gRNA expression in astrocytes

   Depositor

   Ben Deverman

   Article

   Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

   Insert

   NLS-GFP, 2x U6-RNA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5FRT/TO-FAF1-Strep-HA

   Plasmid

   #113486

   Purpose

   Expression of human FAF1 with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   FAF1 (FAF1 Human)
   

   Use

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   wildtype without stop codon

   Promoter

   CMV

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. BcLOV4 fusion tools screening plasmid #2

   Plasmid

   #174512

   Purpose

   Cloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP in a pcDNA3.1 backbone.

   Depositor

   Brian Chow

   Article

   Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

   Insert

   plasmid B [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP
   

   Use

   Tags

   3xFLAG and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. PLL5.0-Anillin ShRNA-Cherry-AnillinFL

   Plasmid

   #187271

   Purpose

   Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin full length

   Depositor

   Alpha Yap

   Article

   Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

   Insert

   Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   U6, CMV

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKK-MBP-TEV

   Plasmid

   #105771

   Purpose

   Expression of your protein of interest in fusion with MBP at the N-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   MBP-TEV

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EFS-DIO-GRAB_NTS1.0

    Plasmid

    #208671

    Purpose

    Expresses the genetically-encoded fluorescent neurotensin (NTS) sensor GRAB_NTS1.0 in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based neurotensin (NTS) sensor GRAB_NTS1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. qTAG-C-Solo-mStayGold-CLTC

    Plasmid

    #227313

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the CLTC locus. For clathrin heavy chain visualization. To be co-transfected with sgRNA px330-PITCh-CLTC (Addgene #227312)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    CLTC Homology Arms flanking a mStayGold Tag (CLTC Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTwist-SARS-CoV-2 Spike-deltaC18 BA.2

    Plasmid

    #212487

    Purpose

    Encodes SARS-CoV-2 variant BA.2 Spike for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike BA.2 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments.

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL3-noSV40-humanCOL2A1enhancer

    Plasmid

    #173961

    Purpose

    Firefly luciferase enhancer reporter plasmid.

    Depositor

    Joanna Wysocka

    Article

    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

    Insert

    Enhancer region from COL2A1 gene
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    None

    Promoter

    SV40 promoter

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-UBC-S3-ExRaiAKAR

    Plasmid

    #160724

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-AKAR (PKA indicator), V5 tag, and S3 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S3-ExRaiAKAR
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-UBC-S4-ExRaiCKAR

    Plasmid

    #160725

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-CKAR (PKC indicator), OLLAS tag, and S4 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S4-ExRaiCKAR
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-GRAB_CRFmut

    Plasmid

    #208659

    Purpose

    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) control sensor GRAB_CRFmut in neurons

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKK-TEV-BirA-FLAG

    Plasmid

    #105786

    Purpose

    Expression of your protein of interest in fusion with BirA-FLAG at the C-terminus (cleavable by TEV). Search for protein partners using the BioID (PMID: 15707685, 22412018).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-BirA-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLentiCrisprV2 sgRNA hPER2c-term #1

    Plasmid

    #179454

    Purpose

    Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

    Depositor

    Achim Kramer

    Article

    Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

    Insert

    sgRNA targeting human PER2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLentiCrisprV2 sgRNA hPER2c-term #2

    Plasmid

    #179455

    Purpose

    Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

    Depositor

    Achim Kramer

    Article

    Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

    Insert

    sgRNA targeting human PER2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLentiCrisprV2 sgRNA hPER2c-term #3

    Plasmid

    #179456

    Purpose

    Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

    Depositor

    Achim Kramer

    Article

    Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

    Insert

    sgRNA targeting human PER2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge