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Showing: 6601 - 6620 of 18117 results

  1. pLenti-CMV-eGFP-hTau E14-WPRE

    Plasmid
    #233042
    Purpose
    To Express a GFP human Tau E14 fusion protein from a CMV promoter
    Depositor
    Jonathan Ploski
    Insert
    eGFP-hTau E14 fusion (MAPT Synthetic, Human)
    Use
    Lentiviral
    Tags
    EGFP
    Available Since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    233042-plasmid-map-sequence-id-463775

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  2. pcDNA3.1-EF1A-mNeonGreen2x-SREBP1-P2A-Puro

    Plasmid
    #228401
    Purpose
    Encodes a N-terminal truncated version of SREBP1 lacking part of the transcriptional activation domain and fused to a tandem of mNeongreen to visualize the import of SREBP1
    Depositor
    Dirk Daelemans
    Article
    Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:
    Insert
    SREBP1 (SREBF1 Human)
    Tags
    mNeonGreen2x
    Expression
    Mammalian
    Mutation
    deleted N terminal activation domain (amino acids…
    Promoter
    EF1a
    Available Since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    228401-plasmid-map-sequence-id-457257

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  3. pAJE-Ma pylRS nitroY/haloY-F5

    Plasmid
    #225684
    Purpose
    Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control during Ma Pyl tRNA-synthetase validation.
    Depositor
    Ryan Mehl
    Inserts
    M. alvus PylRS 3-NY/HaloY F5
    M. alvus Pyl-tRNA(6)
    Use
    Methanomethylophilus alvus
    Mutation
    CTG125CTT, N166A, V168S, A223C, W239R
    Promoter
    GlnS and Lpp
    Available Since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    225684-plasmid-map-sequence-id-450701

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  5. pJAT75

    Plasmid
    #204306
    Purpose
    Flippase under heat shock control. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthparts
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-EGFP
    Use
    CRISPR
    Promoter
    ie1
    Available Since
    Aug. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    204306-plasmid-map-sequence-id-406175

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  6. pJAT25

    Plasmid
    #204310
    Purpose
    For generating targeting inversions. C-terminal split GFP, expressed in abdomen and mouthparts.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-GFPc
    Use
    CRISPR
    Promoter
    ie1
    Available Since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    204310-plasmid-map-sequence-id-405854

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  7. pL1P4 ZmUbiP:GRF-GIF:NosT

    Plasmid
    #198048
    Purpose
    MoClo Golden Gate Level 1 Position 4. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.
    Depositor
    Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing
    Insert
    ZmUbiP::TaGRF4-GIF1::NosT
    Use
    Synthetic Biology
    Promoter
    Zea mays (maize) ubiquitin promoter
    Available Since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    198048-plasmid-map-sequence-id-389382

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  8. pL1P5 ZmUbiP:GRF-GIF:NosT

    Plasmid
    #198046
    Purpose
    MoClo Golden Gate Level 1 Position 5. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.
    Depositor
    Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing
    Insert
    ZmUbiP::TaGRF4-GIF1::NosT
    Use
    Synthetic Biology
    Promoter
    Zea mays (maize) ubiquitin promoter
    Available Since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    198046-plasmid-map-sequence-id-383687

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  9. p663-UBC-miniTurbo-V5-CDK20_IDG-K

    Plasmid
    #189883
    Purpose
    Gateway destination clone of CDK20 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    CDK20 (CDK20 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    189883-plasmid-map-sequence-id-374321

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  10. p663-UBC-miniTurbo-V5-TTBK2_IDG-K

    Plasmid
    #189881
    Purpose
    Gateway destination clone of TTBK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    TTBK2 (TTBK2 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    189881-plasmid-map-sequence-id-374318

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  11. p663-UBC-miniTurbo-V5-NEK4_IDG-K

    Plasmid
    #189875
    Purpose
    Gateway destination clone of NEK4 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    NEK4 (NEK4 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    189875-plasmid-map-sequence-id-374316

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  12. p663-UBC-miniTurbo-V5-NEK8_IDG-K

    Plasmid
    #189877
    Purpose
    Gateway destination clone of NEK8 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    NEK8 (NEK8 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Oct. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    189877-plasmid-map-sequence-id-374131

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  14. pDTFendoNA2

    Plasmid
    #174731
    Purpose
    Expresses DT386F-endoNA2 fusion protein for selective elimination of polysialic acid–containing cells. Truncated diphtheria toxin fused to noncatalytic endosialidase via a furin-cleavable linker.
    Depositor
    Jukka Finne
    Article
    Lehti et al Mol Cancer Ther. 2021 Jul 26. pii: 1535-
    Insert
    DT386F
    Expression
    Bacterial
    Mutation
    Deleted amino acids 412-560 (the receptor binding…
    Available Since
    Sept. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    174731-plasmid-map-sequence-id-343522

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  15. p663-UBC-miniTurbo-V5-CDK19_IDG-K

    Plasmid
    #158175
    Purpose
    Gateway destination clone of CDK19 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-CDK19 (CDK19 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Sept. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    158175-plasmid-map-sequence-id-311145

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  16. p667-UBC-TLK2-V5-TurboID_IDG-K

    Plasmid
    #135250
    Purpose
    Gateway destination clone of TLK2 (human) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    TLK2-V5-TurboID (TLK2 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-TurboID
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Jan. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    135250-plasmid-map-sequence-id-269116

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  17. pCWXPGR-pTF-ERK:MEK

    Plasmid
    #114291
    Purpose
    TET-inducible expression of ERK:MEK fusion protein
    Depositor
    Patrick Salmon
    Insert
    rERK2 and hMEK1 proteins (Mapk1 Human, Rat)
    Use
    Lentiviral
    Mutation
    rat ERK2 fused to human MEK1 with a linker in bet…
    Available Since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114291-plasmid-map-sequence-id-218696

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  18. SMARCA4A

    Plasmid
    #74664
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SMARCA4A (SMARCA4 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  19. AKAP13

    Plasmid
    #73232
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    AKAP13 (AKAP13 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    contains amino acids 1976-2211
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 6601 - 6620 of 18117 results