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HA-MBP-TEVpCS-ADGRB1 CTF-FLAG

Plasmid

#217681

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRB1 (ADGRB1 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-926

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsXRN1_1174-1706_K

Plasmid

#146788

Purpose

Mammalian Expression of HsXRN1_1174-1706

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

HsXRN1_1174-1706 (XRN1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGRCRE

Plasmid

#129116

Purpose

Expresses cre recombinase in Z. rouxii yeast cells

Depositor

Paolo Giudici

Article

Bizzarri et al Yeast. 2019 Dec;36(12):711-722. doi: 10.

Insert

cre

Use

Cre/Lox

Expression

Yeast

Promoter

S. cerevisiae GAL1 promoter

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-CFL-SN-P2A-GCaMP6f

Plasmid

#181742

Purpose

AAV vector for expressing CFL-SN and GCaMP6f

Depositor

Yasunori Hayashi

Article

Goto et al Science. 2021 Nov 12;374(6569):857-863.

Insert

CFL-SN-P2A-GCaMP6f

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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ACPPiso1-V5

Plasmid

#113052

Purpose

Expresses human prostatic acid phosphatase (isoform 1) in mammalian cells

Depositor

Federico Sesti

Article

Cotella et al FASEB J. 2013 Apr;27(4):1381-93. doi: 10

Insert

prostatic acid phosphatase isoform 1 (ACP3 Human)

Tags

V5

Expression

Mammalian

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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ACPPiso2-V5

Plasmid

#113053

Purpose

Expresses human prostatic acid phosphatase (isoform 2) in mammalian cells

Depositor

Federico Sesti

Article

Cotella et al FASEB J. 2013 Apr;27(4):1381-93. doi: 10

Insert

prostatic acid phosphatase isoform 2 (ACP3 Human)

Tags

V5

Expression

Mammalian

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pHB4

Plasmid

#53462

Purpose

This plasmid contains an ADHpr driven chimeric protein with SNC1, N-terminal GFP and C-terminal SUC2. It contains flanking homology to SUC2, allowing optional integration.

Depositor

Elizabeth Conibear

Article

Burston et al J Cell Biol. 2009 Jun 15. 185(6):1097-11

Insert

yeGFP-Snc1-Suc2

Expression

Yeast

Promoter

ADH

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAD-CMV-Caveolin1-CMV-GFP

Plasmid

#83272

Purpose

Adenoviral expression of Caveolin1 with co-expression of GFP

Depositor

Andrew Brooks

Article

Fernandez-Rojo et al Cell Rep. 2013 Jul 25;4(2):238-47. doi:

Inserts

CAV1 (CAV1 Human)
eGFP

Use

Adenoviral

Promoter

CMV

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pEXP5-NT-AlleyCat

Plasmid

#41715

Depositor

William DeGrado, Ivan Korendovych

Article

Korendovych et al Proc Natl Acad Sci U S A. 2011 Apr 26;10

Insert

mutated C-terminal portion of calmodulin gene (M76-K148)

Tags

Polyhistidine (6×His) region, TEV recognition sit…

Expression

Bacterial

Mutation

F92E

Promoter

T7

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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hyBE4max

Plasmid

#157942

Purpose

Mammalian expression of hyBE4max

Depositor

Dali Li

Article

Zhang et al Nat Cell Biol. 2020 Jun;22(6):740-750. d

Insert

hyBE4max

Use

CRISPR

Expression

Mammalian

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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ssAAV-EF1a-FLuc-WPRE-HgHpA_bac_293

Plasmid

#118412

Purpose

Expresses firefly luciferase under the control of a strong ubiquitous promoter EF1a along with a WPRE cassette. Backbone is compatible with both baculoviral and human production methods of AAV.

Depositor

Nicole Paulk

Article

Rumachik et al Mol Ther Methods Clin Dev. 2020 May 22;1

Insert

Firefly luciferase

Use

AAV, Luciferase, and Synthetic Biology

Expression

Insect and Mammalian

Promoter

EF1a

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pKG1313_pPV3-WPRE

Plasmid

#239703

Purpose

BsaI Golden Gate assembly plasmid encoding the WPRE 3' UTR sequence

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

WPRE 3' UTR

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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JAB2296

Plasmid

#190365

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-PhlFO::GFPint-NLS-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_pro35S-PhlFO::GFPint-NLS-ADHt

Expression

Plant

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2603

Plasmid

#190390

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_5XAmtRO::GFPint-NLS-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_5XAmtRO::GFPint-NLS-ADHt

Expression

Plant

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2098

Plasmid

#190340

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-2XPhlFO::GFPint-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-2XPhlFO::GFPint-ADHt

Expression

Plant

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2192

Plasmid

#190356

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XQacRO-min35S::GFPint-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_6XQacRO-min35S::GFPint-ADHt

Expression

Plant

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-V2_mMSH2

Plasmid

#186156

Purpose

sgRNA targeting murine Msh2 gene

Depositor

David McFadden

Article

Povedano et al Cell Chem Biol. 2019 Sep 19;26(9):1315-1

Insert

Msh2_gRNA (Msh2 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pcryBFP_Umyo18bGFP

Plasmid

#172527

Purpose

alphaA-crystallin promoter (cry) drives GFP expression in the lens. The 600bp unc-45b muscle-specific promoter drives expression of GFP-tagged zebrafish Myo18b.

Depositor

Joachim Berger

Article

Berger et al Hum Mol Genet. 2017 Mar 15;26(6):1146-11

Insert

myo18b

Expression

Mammalian

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA

Plasmid

#162802

Purpose

STELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient expression

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-BsmBI-HA

Use

Piggybac

Tags

HA

Expression

Mammalian

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pET28b-pchA WT

Plasmid

#87102

Purpose

expresses PchA WT, the isochorismate synthase from Pseudomonas aeruginosa

Depositor

Audrey Lamb

Article

Meneely et al Arch Biochem Biophys. 2013 Oct 1;538(1):

Insert

pchA WT

Tags

his-tag

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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