We narrowed to 10,134 results for: ada

1. NC14 pGLUE FBXW11

   Plasmid

   #36969

   Depositor

   Randall Moon

   Article

   Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

   Insert

   FBXW11 (FBXW11 Human)
   

   Tags

   CBP tag, HA, and streptag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 10, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pKK-TEV-EGFP

   Plasmid

   #105793

   Purpose

   Expression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 8885998, 8805248). EGFP is not truly monomeric (PMID: 11988576, 22869113)

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-EGFP

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiCRISPRv2_sgCtrl#1

   Plasmid

   #174148

   Purpose

   Lentiviral vector expressing Cas9 and a control sgRNA that targets a safe harbor site

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   Control sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Circular 200,100 (RAB7A)

   Plasmid

   #170118

   Purpose

   AAV vector carrying a guide RNA targeting the human RAB7A mRNA

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 200,100 guide RNA (RAB7A Human)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Human U6

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. CTCF-mAC donor (Neo)

   Plasmid

   #140645

   Purpose

   CTCF tagging with mAID-Clover

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   CTCF (CTCF Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pR26-CMVconst-mBMP7

   Plasmid

   #127375

   Purpose

   Allows for constitutive BMP7 expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.

   Depositor

   Lance Miller

   Article

   Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

   Insert

   BMP7 (Bmp7 Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Promoter

   CMV enhancer/promoter

   Available Since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. qTAG-C-Solo-mStayGold-COX8A

   Plasmid

   #227309

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the COX8A locus. For mitochondria visualization. To be co-transfected with sgRNAplasmid px330-PITCh-COX8A (Addgene #227308)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   COX8A Homology Arms flanking a mStayGold Tag (COX8A Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTwist-SARS-CoV-2 Spike-deltaC18 JN.1.11.1

   Plasmid

   #223272

   Purpose

   Encodes SARS-CoV-2 spike JN.1.11.1 for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike JN.1.11.1 (S Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Mammalian

   Mutation

   See Depositor Comments.

   Promoter

   CAG

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. hU6-pegRNA-EF-1α-Cre Template

   Plasmid

   #196036

   Purpose

   The template UPEC vector designed for cloning of a single pegRNA and subsequent co-expression with Cre.

   Depositor

   Tyler Jacks

   Article

   Ely et al Nat Biotechnol. 2023 May 11. doi: 10.103

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   hU6 and EF-1-alpha

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. hSyn-GtACR2-ChRmine-tandem-eYFP

    Plasmid

    #192580

    Purpose

    AAV-vector for bidirectional optogenetic manipulation of neurons

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    EYFP- soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Promoter

    human synapsin

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. qTAG-C-mNeon-Puro-TOMM20

    Plasmid

    #207790

    Purpose

    Donor template for mNeon-2A-Puro insertion into the C-terminus of the TOMM20 locus. For mitochondria visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TOMM20 (Addgene #207789)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TOMM20 Homology Arms flanking a mNeon-Puro Cassette (TOMM20 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Flag-M4-AR-S650D

    Plasmid

    #171246

    Purpose

    Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser650Asp (AR-S650D)

    Depositor

    Steven Balk

    Article

    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

    Insert

    3xFlag-tagged human androgen receptor Ser650Asp mutant (AR Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AR-Ser650Asp (AR-S650D)

    Promoter

    CMV

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Flag-M4-AR-S650A

    Plasmid

    #171245

    Purpose

    Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser650Ala (AR-S650A)

    Depositor

    Steven Balk

    Article

    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

    Insert

    3xFlag-tagged human androgen receptor Ser650Ala mutant (AR Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AR-Ser650Ala (AR-S650A)

    Promoter

    CMV

    Available Since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCI-neo-RL-ZEB1 200bmutx5

    Plasmid

    #35537

    Depositor

    Greg Goodall

    Article

    Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

    Insert

    3'UTR of ZEB1 (ZEB1 Budding Yeast, Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    5 mutations in miR200b seed regions of 3'UTR…

    Available Since

    May 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKHH030_sgCtrl#1

    Plasmid

    #174142

    Purpose

    Lentiviral vector expressing a control sgRNA that targets a safe harbor site

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-DIO-GRAB_VIP1.0

    Plasmid

    #208680

    Purpose

    Expresses the genetically-encoded fluorescent vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0 in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-SaPE2-P2A-BSD

    Plasmid

    #174818

    Purpose

    Mammalian expression of SaCas9 prime editor 2 with P2A-BSD marker

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    SaPE2-P2A-BSD
    

    Tags

    SV40 bpNLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. qTAG-N-Blast-3xFLAG-LMNB1

    Plasmid

    #207777

    Purpose

    Donor template for Blast-2A-3xFLAG insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    LMNB1 Homology Arms flanking a Blast-3xFLAG Cassette (LMNB1 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 29, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pPB-cT3G-cERP2-NR2F2

    Plasmid

    #192900

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of NR2F2

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    NR2F2 (NR2F2 Human)
    

    Expression

    Mammalian

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. NLS-4LNDD-pcDNA3.1(+)

    Plasmid

    #172313

    Purpose

    Expresses ADAR2 Deaminase Domain (DD) fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS) in mammalian cells

    Depositor

    Joshua Rosenthal

    Article

    Vallecillo-Viejo et al RNA Biol. 2018 Jan 2;15(1):104-114. doi:

    Insert

    Lambda N peptide - human ADAR2 Deaminase Domain
    

    Tags

    6xHis, Flag, and Nuclear Localization Signal of S…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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