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pLKO_HSV.NWS.S_mCherry-NLS

Plasmid

#178273

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

HSV.NWS.S and Nuclear Localization Signal

Expression

Mutation

Promoter

eF1a

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-Drosha1-390

Plasmid

#62521

Purpose

C-terminal aa391-1374 of Drosha is deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with aa 391-1374 deleted (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 391-1374

Promoter

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only
VP16-ER beta (short)

Plasmid

#11353

Depositor

Donald McDonnell

Article

Chang et al Mol Cell Biol. 1999 Dec . 19(12):8226-39

Insert

ER beta short (ESR2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2006

Availability

Academic Institutions and Nonprofits only
PET-SpCas9-HF1-plus-NLS-6xHis

Plasmid

#207381

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

SpCas9-HF1-plus

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N497A, Q695A, Q926A, amino acids 1005-1013 replac…

Promoter

T7

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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PET-B-SpCas9-HF1-NLS-6xHis

Plasmid

#207385

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-SpCas9-HF1

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N497A, R661A, Q695A, Q926A, amino acids 1005-1013…

Promoter

T7

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pHS0578 CRISPR associated IscB non-coding locus in pColA-Duet

Plasmid

#176535

Purpose

Non-protein coding locus components from CRISPR-associated IscB

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

non-coding locus components from Delaware Bay metagenome CRISPR-associated IscB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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EF1a_DIO_R133C-HaloTag

Plasmid

#164064

Purpose

Double-Floxed Inverted Open reading frame for mouseMeCP2(alpha) carrying R133C mutation

Depositor

Nathaniel Heintz

Article

Piccolo et al Elife. 2019 Dec 23;8. pii: 51449. doi: 1

Insert

MeCP2 Isoform 1 R133C (Mecp2 Mouse)

Use

AAV

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Ctdnep1_D67E-2xFlag

Plasmid

#196519

Purpose

Expression of Ctdnep1_D67E-2xFlag

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Ctdnep1_D67E-2xFlag (CTDNEP1 Human)

Use

Tags

2xFlag

Expression

Mammalian

Mutation

D67E mutation (phosphastase dead)

Promoter

CMV promoter; T7 promoter

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+CeGFP-Ctdnep1WTsiR

Plasmid

#196517

Purpose

Expression of Ctdnep1WT-GFP

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Ctdnep1WTsiR (CTDNEP1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Silent mutations for siRNA resistance

Promoter

CMV promoter; T7 promoter

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCWXPGR-pTF-DVL2deltaDIX

Plasmid

#114276

Purpose

TET-inducible expression of DVL2 protein with a deletion of its DIX-domain

Depositor

Patrick Salmon

Article

Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

Insert

DVL2 (Dvl2 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

deletion of DIX domain

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-Klf4

Plasmid

#136613

Purpose

Dox-inducible lentiviral vector expressing mouse Klf4

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Klf4 (Klf4 Mouse)

Use

Lentiviral

Tags

Expression

Bacterial

Mutation

Promoter

tetO-miniCMV (dox-inducible)

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-APPL1-R146A/K152A/R154A

Plasmid

#59767

Purpose

Expresses APPL1 Endosomal Localization Mutant

Depositor

Donna Webb

Article

Broussard et al Mol Biol Cell. 2012 Apr;23(8):1486-99. d

Insert

APPL1 (APPL1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

R146A/K152A/R154A

Promoter

CMV

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pJWK_VD_01

Plasmid

#158102

Purpose

5'UTR stem-loop luciferase reporter: 0kcal/mol

Depositor

Ted Dawson

Article

Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

Insert

5'UTR synthetic stem-loop 0kcal/mol

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-sCreDIO hChR2(H134R)-EYFP

Plasmid

#55642

Purpose

sCre-Dependent ChR2-EYFP

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

hChR2(H134R)-EYFP

Use

AAV; Scre-dependent

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Aug. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pYPQ239-RR

Plasmid

#108862

Purpose

FnCpf1 Gateway entry plasmid with N623R and K687R mutations

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

Insert

FnCpf1-RR (FnCpf1 with N623R and K687R mutations)

Use

CRISPR; Gateway compatible cpf1 entry clone

Tags

NLS

Expression

Plant

Mutation

N623R and K687R, Cpf1 is rice codon optimized

Promoter

Available since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV_UdgX-EE-UdgX-NG-nCas9-RBMX

Plasmid

#163571

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-EE-UdgX-NG-nCas9-RBMX

Use

CRISPR

Tags

Expression

Mammalian

Mutation

nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

Promoter

Available since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-LC3-K51A

Plasmid

#155265

Purpose

Mammalian expression of rat LC3 fused to EGFP

Depositor

David Rubinsztein

Article

Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Tags

pEGFP

Expression

Mammalian

Mutation

Lysine 51 to Alanine

Promoter

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-LC3-R70A

Plasmid

#155264

Purpose

Mammalian expression of rat LC3 fused to EGFP

Depositor

David Rubinsztein

Article

Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Tags

pEGFP

Expression

Mammalian

Mutation

Arginine 70 to Alanine

Promoter

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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Rat Alpha2 delta-1 mutMIDAS pMT2

Plasmid

#58730

Purpose

expression of rat alpha2 delta-1 with MIDAS site mutations in mammalian cells

Depositor

Annette Dolphin

Article

Hoppa et al Nature. 2012 May 13;486(7401):122-5. doi

Insert

alpha2 delta-1 (Cacna2d1 Rat)

Use

Tags

Expression

Mammalian

Mutation

MIDAS site mutations (D259VSGS to AVAGA)

Promoter

SV40

Available since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only

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OCT4 GFP puro Donor 3

Plasmid

#22211

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

SA-OCT-GFP-2APuro-PA (POU5F1 Human)

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

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