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pJUMP19-B0015_T

Plasmid

#127038

Purpose

Basic Part T- Terminator; B0015. Terminator.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part B0015_T

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP18-Histag_N

Plasmid

#127033

Purpose

Basic Part N- N-terminus; polyhistidine tag

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part His6_N

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP18-cI-ts_O

Plasmid

#127030

Purpose

Basic Part O- ORF; cI; transcription factor from lambda phage; thermosensitive.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part cI-ts_o

Use

Synthetic Biology

Expression

Bacterial

Mutation

cI thermosensitive mutation

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP18-cI_O

Plasmid

#127029

Purpose

Basic Part O- ORF; cI; transcription factor from lambda phage.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part cI_o

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP18-lacZ_O

Plasmid

#127027

Purpose

Basic Part O- ORF; lacZ alpha-peptide; blue/white screening.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part lacZ_o

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP18-lacI_O

Plasmid

#127024

Purpose

Basic Part O- ORF; lacI; transcription factor

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part LacI_o

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP18-arsR_O

Plasmid

#127022

Purpose

Basic Part O- ORF; arsR; transcription factor

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part arsR_o

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP18-araC_O

Plasmid

#127020

Purpose

Basic Part O- ORF; araC; transcription factor

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part araC_o

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP18-amilCP_O

Plasmid

#127019

Purpose

Basic Part O- ORF; amilCP; blue chromoprotein

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part amilCP_O

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP18-sfGFP_O

Plasmid

#127018

Purpose

Basic Part O- ORF; sfGFP; fluorescent protein

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part sfGFP_o

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP18-RBS-pET_R

Plasmid

#127013

Purpose

Basic Part R- RBS; pET vector Ribosome Binding Site.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part RBS-pET_R

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP18-B0034_R

Plasmid

#127012

Purpose

Basic Part R- RBS; B0034m Ribosome Binding Site.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part B0034m_R

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP18-B0033_R

Plasmid

#127011

Purpose

Basic Part R- RBS; B0033m Ribosome Binding Site.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part B0033m_R

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP18-B0032_R

Plasmid

#127010

Purpose

Basic Part R- RBS; B0032m Ribosome Binding Site.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part B0032m_R

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP19-Pmer_P

Plasmid

#127008

Purpose

Basic Part P- Promoter (non-constitutive); Pmer; metal sensitive; controlled by merR

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part Pmer_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP19-Pars_P

Plasmid

#127007

Purpose

Basic Part P- Promoter (non-constitutive); Pars; metal sensitive; controlled by arsR

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part Pars_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP19-Pci_P

Plasmid

#127006

Purpose

Basic Part P- Promoter (non-constitutive); Pci; controlled by cI.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part Pci_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP19-Ptet_P

Plasmid

#127005

Purpose

Basic Part P- Promoter (non-constitutive); Ptet; induced by tetracycline/analogue; controlled by TetR

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part Ptet_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP19-J23119s_P

Plasmid

#127001

Purpose

Basic Part P- Promoter (constitutive); J23119 (1bp short on 3').

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part J23119s_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP19-J23106_P

Plasmid

#126999

Purpose

Basic Part P- Promoter (constitutive); J23106.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part J23106_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP19-J23103_P

Plasmid

#126998

Purpose

Basic Part P- Promoter (constitutive); J23103.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part J23103_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
YSBE415: pTOP_ACA1_Vc_HOG1 terminator

Plasmid

#122434

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; C-terminal fragment of Venus protein

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

C-terminal fragment of Venus protein

Use

Tagging proteins in cryptococcus neoformans

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
YSBE416: pTOP_CAC1_Vn_HOG1 terminator

Plasmid

#122435

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; N-terminal fragment of Venus protein

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

N-terminal fragment of Venus protein

Use

Tagging proteins in cryptococcus neoformans

Available Since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCuHA-ATG13[3SD](426)

Plasmid

#122077

Purpose

Expressing HA-ATG13[3SD]

Depositor

Daniel Klionsky

Article

Chew et al Autophagy. 2015;11(6):891-905. doi: 10.1

Insert

ATG13

Tags

3HA

Expression

Yeast

Mutation

Serine 646 to aspartic acid; Serine 649 to aspart…

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pClodANL-FGF2NBAA

Plasmid

#107873

Purpose

Bacterial expression of FGF2 minimum export mutant-NanoLuc

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Insert

FGF2-minimal export

Use

Luciferase

Tags

NanoLuc

Expression

Bacterial

Mutation

C77A, C95A, K128Q, R129Q, K134Q

Promoter

pro D

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS930

Plasmid

#123054

Purpose

Beta-estradiol inducible DNA binding domain

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Z3E [3a]

Expression

Yeast

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGFP-ATG13[3SD](315)

Plasmid

#122074

Purpose

Expressing GFP-Atg13[3SD]

Depositor

Daniel Klionsky

Article

Chew et al Autophagy. 2015;11(6):891-905. doi: 10.1

Insert

ATG13

Tags

GFP

Expression

Yeast

Mutation

Serine 646 to aspartic acid; Serine 649 to aspart…

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGFP-ATG13[3SA](315)

Plasmid

#122073

Purpose

Expressing GFP-Atg13[3SA]

Depositor

Daniel Klionsky

Article

Chew et al Autophagy. 2015;11(6):891-905. doi: 10.1

Insert

ATG13

Tags

GFP

Expression

Yeast

Mutation

Serine 646 to alanine; Serine 649 to alanine; Ser…

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF704

Plasmid

#121658

Purpose

U6-sgRNA EFS-ProCas9-Flavi-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

Cas9(C)

Use

Lentiviral

Promoter

U6

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF712

Plasmid

#121663

Purpose

U6-sgRNA EF1a-ProCas9-Flavi-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

Cas9(C)

Use

Lentiviral

Promoter

U6

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge