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pLVX-EF1alpha-SARS-CoV-2-orf8-2xStrep-IRES-Puro

Plasmid

#141390

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf8 (ORF8 SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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GFP-Ub KO

Plasmid

#11934

Depositor

Nico Dantuma

Article

Bergink et al Genes Dev. 2006 May 15. 20(10):1343-52.

Insert

Ubiquitin KO (RBX1 Human)

Tags

GFP

Expression

Mammalian

Mutation

All 7 lysines of ubiquitin mutated to arginines.

Available Since

Aug. 7, 2006

Availability

Academic Institutions and Nonprofits only

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VCP(R155H)-EGFP

Plasmid

#23972

Depositor

Nico Dantuma

Article

Tresse et al Autophagy. 2010 Feb 22. 6(2):.

Insert

Valosin containing protein (VCP Human)

Tags

6xHis, EGFP, and HA

Expression

Mammalian

Mutation

R155H

Available Since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

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pCGN-ATF6 (1-373)

Plasmid

#27173

Purpose

For mammalian expression of the cytoplasmic domain of human ATF6with an HA tag.

Depositor

Ron Prywes

Article

Wang et al J Biol Chem. 2000 Sep 1. 275(35):27013-2

Insert

ATF6 (ATF6 Human)

Tags

HA

Expression

Mammalian

Mutation

Contains only aa 1-373 (please see depositor comm…

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV10-hFIP200

Plasmid

#24300

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

FIP200 (RB1CC1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP7b

Plasmid

#104484

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

Douglas Kim, GENIE Project

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Insert

jGCaMP7b

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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SaBE4-Gam

Plasmid

#100809

Purpose

Expresses SaBE4-Gam in mammalian cells

Depositor

David Liu

Article

Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

Insert

SaBE4-Gam

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag C/EBPa

Plasmid

#66978

Purpose

Expression plasmid for mouse C/EBPa; adds Flag tag to the N-terminus of protein

Depositor

Christopher Vakoc

Article

Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

Insert

C/EBP alpha (Cebpa Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

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MSCV-BCL6-IRES-GFP

Plasmid

#31391

Depositor

Hilda Ye

Article

Yu et al Blood. 2005 Feb 15. 105(4):1777-84.

Insert

B-cell lymphoma 6 (BCL6 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pLV-EBFP2-nuc

Plasmid

#36085

Depositor

Pantelis Tsoulfas

Type

Empty backbone

Use

Lentiviral

Tags

NLS

Expression

Mammalian

Mutation

Replace EYFP of the vector pEYFP-Nuc from Clontec…

Available Since

June 1, 2012

Availability

Academic Institutions and Nonprofits only

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