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pPV-C1-crRNA-cloning-EF1a-BA

Plasmid

#204623

Purpose

piggyBac vector for crRNA cloning and expression. Blasticidin selectable.

Depositor

Akitsu Hotta

Article

Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.17_GagZip-MCP-dpol-P2A-eGFP

Plasmid

#205532

Purpose

Express HIV GagZip-MCP-dpol-P2A-eGFP

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

Gag-MCP-dpol

Expression

Mammalian

Mutation

Nucleocapsid replaced with leucine zipper, pol fr…

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.98_PB-EPN1-MCP_Myc-T2A-eGFP-PGK-Blast

Plasmid

#205543

Purpose

Express EPN1-MCP-Myc-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN1-MCP-Myc

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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CMV EGFP-PPM1H-H153D

Plasmid

#200432

Purpose

Mammalian expression of H153D mutation in PPM1H

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

PPM1H protein phosphatase, Mg2+/Mn2+ dependent 1H

Tags

EGFP

Expression

Mammalian

Mutation

H153D

Promoter

CMV

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-6-AmCyan-P2A-NptII-9

Plasmid

#197742

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

AmCyan-P2A-NptII bicistronic CDS

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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1K245-->R GLUT1-GFP

Plasmid

#200095

Purpose

1K245--> GLUT1 mutant with C-terminal GFP tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

GLUT1 (SLC2A1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

K245R

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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TXNIP-py-FLAG

Plasmid

#200092

Purpose

TXNIP py motif mutant with C-terminal 3xFLAG tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

TXNIP (TXNIP Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

py motifs mutated (aa331-334 and 375-378)

Promoter

CMV

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL1954

Plasmid

#195689

Purpose

Expression/purification of 6xHis-TEV-MlaD(F118A/L123A)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaD

Expression

Bacterial

Mutation

6xHis-TEV-MlaD(F118A/L123A)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL1936

Plasmid

#195692

Purpose

Complementation of MlaF-MlaE-MlaDdelta153-183-MlaC-MlaB

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE-MlaDdelta153-183-MlaC-MlaB

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL1935

Plasmid

#195693

Purpose

Complementation of MlaF-MlaE-MlaDdelta173-183-MlaC-MlaB

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE-MlaDdelta173-183-MlaC-MlaB

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL1953

Plasmid

#195694

Purpose

Complementation of MlaF-MlaE-MlaD(F118A/L123A)-MlaC-MlaB

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE-MlaD(F118A/L123A)-MlaC-MlaB

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL1933

Plasmid

#195695

Purpose

Complementation of MlaF-MlaE-MlaD(F118A)-MlaC-MlaB

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE-MlaD(F118A)-MlaC-MlaB

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL1934

Plasmid

#195696

Purpose

Complementation of MlaF-MlaE-MlaD(L123A)-MlaC-MlaB

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE-MlaD(L123A)-MlaC-MlaB

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2707

Plasmid

#195636

Purpose

Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

sfGFP-MlaA

Expression

Bacterial

Mutation

6xHis-TEV-sfGFP-MlaA (227-251)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2753

Plasmid

#195637

Purpose

Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; I241N)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

sfGFP-MlaA

Expression

Bacterial

Mutation

6xHis-TEV-sfGFP-MlaA (227-251; I241N)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2754

Plasmid

#195638

Purpose

Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; L245N)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

sfGFP-MlaA

Expression

Bacterial

Mutation

6xHis-TEV-sfGFP-MlaA (227-251; L245N)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2755

Plasmid

#195639

Purpose

Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; I248N)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

sfGFP-MlaA

Expression

Bacterial

Mutation

6xHis-TEV-sfGFP-MlaA (227-251; I248N)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2756

Plasmid

#195640

Purpose

Expression/purification of 6xHis-TEV-sfGFP-MlaA(227-251; delta238-251)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

sfGFP-MlaA

Expression

Bacterial

Mutation

6xHis-TEV-sfGFP-MlaA(227-251; delta238-251)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2757

Plasmid

#195641

Purpose

Expression/purification of 6xHis-TEV-sfGFP-MlaA(227-251; delta244-251)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

sfGFP-MlaA

Expression

Bacterial

Mutation

6xHis-TEV-sfGFP-MlaA(227-251; delta244-251)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2766

Plasmid

#196028

Purpose

Complementation of MlaF-MlaE-MlaDdelta141-183-MlaC-MlaB

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE-MlaDdelta141-183-MlaC-MlaB

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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