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4xHRE_miniTK-sfGFP-CMV_dsRed Exp

Plasmid

#117404

Purpose

sfGFP expressed from hypoxia-inducible miniTK promoter and dsRed expressed from consitutive CMV promoter

Depositor

Yvonne Chen

Article

Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

Insert

SuperFolder GFP

Expression

Mammalian

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Laccase2 Sense

Plasmid

#69892

Purpose

Expresses the Drosophila laccase2 exon 2 circular RNA

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

Laccase2 (stw Fly)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pBS ZfHoxd3a

Plasmid

#27534

Depositor

Victoria Prince

Article

Prince et al Development. 1998 Feb . 125(3):393-406.

Insert

Hoxd3a (hoxd3a Zebrafish)

Use

Cloning vector

Available Since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only
Mix.2-lux M-p53BE

Plasmid

#20894

Depositor

Stefano Piccolo

Article

Cordenonsi et al Cell. 2003 May 2. 113(3):301-14.

Insert

-290 Mix.2 M-p53BE promoter luciferase

Expression

Mammalian

Mutation

point mutation of the p53 binding element (p53BE)…

Available Since

April 22, 2009

Availability

Academic Institutions and Nonprofits only
pRSM15

Plasmid

#101640

Purpose

Encodes the fluorescent protein EG-4 for constitutive expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

EG-4

Tags

6XHis

Expression

Bacterial

Available Since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

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Ube2i

Plasmid

#31420

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Ubiquitin Conjugating Enzyme (UBE2I Human)

Tags

HA

Expression

Yeast

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
hopscotch

Plasmid

#37284

Depositor

Norbert Perrimon

Article

Binari et al Genes Dev. 1994 Feb 1;8(3):300-12.

Insert

hopscotch (hop Fly)

Expression

Bacterial

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pCVD447

Plasmid

#78569

Purpose

E. coli E2348/69 LEE region (Type 3 Secretion System) cloned into pBluescriptKS.

Depositor

James Kaper

Article

Jarvis et al Proc Natl Acad Sci U S A. 1995 Aug 15;92

Insert

lee operon

Use

Cloning vector

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only
pSG 415

Plasmid

#63862

Purpose

Detect translation initiation from AUC start codon (E. coli lacZ)

Depositor

Albert Dahlberg

Article

O'Connor et al Nucleic Acids Res. 1997 Mar 15;25(6):118

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

AUC start codon

Promoter

PtacI

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only
pSG 414

Plasmid

#63861

Purpose

Detect translation initiation from UUG start codon (E. coli lacZ)

Depositor

Albert Dahlberg

Article

O'Connor et al Nucleic Acids Res. 1997 Mar 15;25(6):118

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

UUG start codon

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pSG 431

Plasmid

#63866

Purpose

Detect translation initiation from GUG start codon (E. coli lacZ)

Depositor

Albert Dahlberg

Article

O'Connor et al Nucleic Acids Res. 1997 Mar 15;25(6):118

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

GUG start codon

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pET12a-AL-09 FL H87Y C214S

Plasmid

#47087

Depositor

Marina Ramirez-Alvarado

Article

Levinson et al Sci Rep. 2013;3:1278. doi: 10.1038/srep0

Insert

AL-09 FL H87Y C214S (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Mutation

H87Y C214S

Promoter

T7

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pCR8-DJ1-D149A

Plasmid

#29355

Depositor

Mark Cookson

Article

Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

Insert

DJ1 (PARK7 Human)

Use

Entry vector

Mutation

Asp149Ala

Available Since

March 31, 2011

Availability

Academic Institutions and Nonprofits only
pTTSTOP

Plasmid

#41751

Depositor

Jeff Gelles

Article

Thoresen et al Biochemistry. 2008 Sep 9;47(36):9514-21.

Insert

kinesin heavy chain (Khc Fly)

Expression

Bacterial

Mutation

residues 1-401

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pEN_TmiR_G13-L

Plasmid

#25762

Purpose

Entry vector with TRE promoter driving mouse G alpha 13 miR30-based shRNA.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 13 miR-shRNA

Use

Entry vector

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO V5 DNAJB3

Plasmid

#19525

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DnaJ (Hsp40) homolog, subfamily B, member 3 (DNAJB3 Mouse)

Tags

V5

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pM2_J23102_R1_LuxR

Plasmid

#250887

Purpose

LuxR regulator protein expressed under BBa_J23102 promoter; part of BNeu AS4, AS8, AS9 and AS14; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

LuxR

Use

Synthetic Biology

Promoter

BBa_J23102

Available Since

March 5, 2026

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-HNF1A-PURO

Plasmid

#251734

Purpose

Overexpression construct expressing HNF1A-PURO and ORF-BC

Depositor

Andrea Califano

Insert

HNF1A (HNF1A Human)

Use

Lentiviral

Available Since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2_RFP_2xRecEnhancedEdited_1xBoxB_EYFP

Plasmid

#250546

Purpose

In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of a flourenscent reporter containing a box-b hairpin without two editible stop codons

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

RFP_2xRecEnhancedEdited_1xBoxB_EYFP

Use

Synthetic Biology

Available Since

Feb. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pM2_PJ23102R1LuxR_PCTR1E2Crimson

Plasmid

#250892

Purpose

LuxR expressed under BBa_J23102; E2-Crimson under PCT synthetic promoter; component of BNeuAS6; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Inserts

E2-Crimson
LuxR

Use

Synthetic Biology

Promoter

BBa_J23102 and PCT

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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