We narrowed to 15,601 results for: LEA

1. pcDNA3-AKAP79-AKAR4

   Plasmid

   #181957

   Purpose

   Genetically encoded FRET-based PKA activity reporter fused to AKAP79.

   Depositor

   Jin Zhang

   Article

   Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

   Insert

   AKAP79-AKAR4 (AKAP5 Human)
   

   Use

   Tags

   AKAP79, Cerulean, HIS tag, and cpVenus(E172)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmCherry-Gal3

   Plasmid

   #85662

   Purpose

   Fluorescent reporter for lysosomal damage

   Depositor

   Hemmo Meyer

   Article

   Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

   Insert

   LGALS3 (LGALS3 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Ef1a-fDIO hChR2(H134R)-EYFP

   Plasmid

   #55639

   Purpose

   Flp-Dependent ChR2-EYFP

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde

   Insert

   hChR2(H134R)-EYFP
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Aug. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-MMLVgag-3xNES-Cas9

   Plasmid

   #181752

   Purpose

   MMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.

   Depositor

   David Liu

   Article

   Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

   Insert

   MMLV-Gag-3xNES-Cas9 (Spy)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSFDuet-1_VNp-mNeongreen-Erythropoietin

   Plasmid

   #182423

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-mNeongreen-Erythropoietin fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp-mNeongreen-eythropoietin (EPO Human)
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp) & mNeongreen

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmCherry-N1-EXO1b

   Plasmid

   #111621

   Purpose

   Expression of human WT Exonuclease 1 (EXO1) tagged with mCherry in mammalian cells

   Depositor

   Marco Muzi-Falconi

   Article

   Sertic et al Mol Cell. 2018 Apr 5;70(1):34-47.e4. doi

   Insert

   Exonuclease 1b (EXO1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. EF.delmFasL.CMV.GFP

   Plasmid

   #17620

   Depositor

   Linzhao Cheng

   Article

   Yu et al Mol Ther. 2003 Jun . 7(6):827-38.

   Insert

   delFasL (Fasl Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Noncleavable mouse delFasL . Deleted the exon 2 r…

   Promoter

   Available since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-HA-LMW-FGF2

   Plasmid

   #157663

   Purpose

   Expresses FGF2 in mammalian cell

   Depositor

   Byung Il Lee

   Article

   Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

   Insert

   Fibroblast growth factor 2 (FGF2 Human)
   

   Use

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   deleted amino acid 1-134

   Promoter

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMGF196

   Plasmid

   #97005

   Purpose

   Expresses LEMD2-mCherry in mammalian cells under a crippled promoter

   Depositor

   Wesley Sundquist, Adam Frost

   Article

   Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   LEMD2 (LEMD2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV delta 5

   Available since

   Aug. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSynSRE-T-Luc

    Plasmid

    #60444

    Purpose

    Uses the hamster HMG-CoA synthase promoter sequence (-324/-225) and minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent transcriptional activation.

    Depositor

    Timothy Osborne

    Article

    Dooley et al J Biol Chem. 1998 Jan 16;273(3):1349-56.

    Insert

    HMG-CoA synthase promoter (-324/-225)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    HMG-CoA synthase (-324/-225) & TATAA (-28/+39)

    Available since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLentiCRISPRv2_cGAS_gRNA_3

    Plasmid

    #235531

    Purpose

    gRNA against human cGAS

    Depositor

    Nicolas Manel

    Article

    Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

    Insert

    cGAS (CGAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTarget

    Plasmid

    #214052

    Purpose

    Expresses target sequence for Haliangium type III CRISPR-Cas complex; identical to pNon-target (Addgene plasmid # 214053) but contains a protospacer.

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    Target sequence
    

    Use

    Target rna plasmid

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHochTypeIII

    Plasmid

    #214039

    Purpose

    Bacterial expression plasmid for Haliangium ochraceum type III CRISPR-Cas complex; contains csb2, a minimal CRISPR array with a pTarget spacer, cmr1-6

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    Cas10
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT

    Promoter

    T7 promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-EF1a-mCherry-IRES-Dre

    Plasmid

    #55633

    Purpose

    Expresses Dre in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Inserts

    mCherry
    Dre
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a and Ef1a/IRES

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3 TFAM-mScarlet

    Plasmid

    #129573

    Purpose

    Expression of mScarlet fused TFAM

    Depositor

    Stephen Tait

    Article

    Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

    Insert

    TFAM (TFAM Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIA33

    Plasmid

    #120803

    Purpose

    E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp

    Depositor

    Craig Ellermeier, David S. Weiss

    Article

    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-

    Inserts

    deactivated nuclease dCas9, codon-optimized for C. difficile
    single guide RNA targeting red fluorescent protein
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Base-pairing region targets red fluorescent prote…

    Promoter

    Pgdh and Pxyl

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX-dL5**-mCER-TRF1

    Plasmid

    #168176

    Purpose

    Expresses FAP (dL5**) fused to mCerulean and the telomere binding protein TRF1

    Depositor

    Patricia Opresko

    Article

    Fouquerel et al Mol Cell. 2019 Jul 11;75(1):117-130.e6.

    Insert

    TRF1 (TERF1 Human)
    

    Use

    Lentiviral

    Tags

    FAP (dL5**) and mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3 TFAM-mClover

    Plasmid

    #129574

    Purpose

    Expression of mClover fused TFAM

    Depositor

    Stephen Tait

    Article

    Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

    Insert

    TFAM (TFAM Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. TVBB N-term-mTurquoise2

    Plasmid

    #169222

    Purpose

    Targeting vector backbone to support a knock-in of mTurquoise2-Linker at the N-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mTurquoise2
    

    Use

    Targeting vector backbone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5-FRT/TO-3*Flag-APEX2 C-term PML

    Plasmid

    #187583

    Purpose

    Express FLAG epitope and APEX2-tagged PML fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    PML (PML Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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