We narrowed to 15,395 results for: jun
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Plasmid#179445PurposeDonor vector to knock in Halotag C-terminal to human CRY1 geneDepositorAvailable SinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only
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pUFlip-floxed2A-KalTA4-; HE1A:BFP -2
Plasmid#180012PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-KalTA4; HE1A: BFP
UseSynthetic BiologyAvailable SinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
R620-M12-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 B.1.351
Plasmid#170196PurposeMammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.351 variant)DepositorInsertSARS-CoV-2 S (spike) B.1.351 (S SARS-CoV-2)
Tags3C-His8-Strep2x2ExpressionMammalianMutationL18F, D80A, D215G, Δ(242-244), R246I, K417N, E484…PromoterCMV51Available SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330 _HITI donor B6
Plasmid#172847PurposeDRS-2 sgRNA expression under a U6 promotor and HITI donor B6 (Zhong et al, eLife 2021), mEGFP translational phase (1-1), excised by DRS-2 sgRNA (with SpCas9).DepositorInsertDRS-2 sgRNA and HITI donor (phase 1) encoding mEGFP
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_Actb sgRNA / hSpCas9
Plasmid#172832PurposeMammalian expression of a sgRNA targeting the intron 1of Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of Actb under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_TOMM20 sgRNA / hSpCas9
Plasmid#172836PurposeMammalian expression of a sgRNA targeting the intron 4 (last intron) of TOMM20 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 4 of TOMM20 under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-P1-like
Plasmid#173789PurposeMammalian expression of SARS-CoV-2 Spike protein P-1-like variant (K417N)DepositorInsertSpike S-GSAS- P-1-like variant (K417N) (S Severe acute respiratory syndrome coronavirus 2)
TagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…Available SinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCsy3-VPR-Csy4
Plasmid#153943PurposeExpresses type I-F P. aeruginosa cascade (PaeCascade) proteins Csy3-VPR and Csy4 in mammalian cellsDepositorInsertCsy3-VPR, Csy4
UseAAV and CRISPRExpressionMammalianPromoterCMVAvailable SinceAug. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCsy1-Csy2
Plasmid#153942PurposeExpresses type I-F P. aeruginosa cascade (PaeCascade) proteins Csy1 and Csy2 in mammalian cellsDepositorInsertCsy1, Csy2
UseAAV and CRISPRTagsFlagExpressionMammalianPromoterCMVAvailable SinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DDX43-K292A
Plasmid#134575PurposeExpress human DDX43 mutant (K292A, ATPase dead) in E. coliDepositorInsertDDX43-K292A (DDX43 Human)
TagsHisExpressionBacterialMutationfull-length (K292A)PromoterT7Available SinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+ Flag-RAGB99L
Plasmid#128101PurposeExpresses constitutively GTP-bound RAGB in mammalian cells in a G418-selectable plasmidDepositorInsertRas related GTP binding B (RRAGB Human)
TagsFlagExpressionMammalianMutationQ99L (CAA to CTA)PromoterCMVAvailable SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pS6-TGFB2-STREP
Plasmid#128503PurposeExpresses human pro-TGFβ2 (from L21 to S414) in mammalian cells. With N-t STREP(2x) tag.DepositorAvailable SinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIE-hα2M-H6
Plasmid#128492PurposeExpresses human α2M (from S24 to N1473) in insect cells. With C-t H6x tag.DepositorAvailable SinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-25-3p
Plasmid#103375PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-25-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-222-3p
Plasmid#103367PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-222-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-222-3p target (MIR222 Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-223-3p
Plasmid#103369PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-223-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-223-3p target (MIR223 Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-23a-3p
Plasmid#103371PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-23a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-23a-3p target (MIR23A Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-183-5p
Plasmid#103285PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-183-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-183-5p target (MIR183 Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-148a-3p
Plasmid#103250PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-148a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-148a-3p target (MIR148A Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-155-3p
Plasmid#103264PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-155-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-155-3p target (MIR155 Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only