We narrowed to 30,195 results for: REP

Showing: 6761 - 6780 of 30195 results

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pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-W

Plasmid

#67986

Purpose

Cas9 activity reporter with mCherry and BFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Inserts

U6gRNA cassette, PGKmCherryABFP cassette, WPRE
Guide RNA targeting modified BFP

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE, modified BFP

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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Anti-Kv7.2/KCNQ2 K+ channel [N26A/23R]

Plasmid

#177524

Purpose

Mammalian Expression Plasmid of anti-Kv7.2/KCNQ2 K+ channel (Human). Derived from hybridoma N26A/23.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Kv7.2/KCNQ2 K+ channel (Homo sapiens) recombinant mouse monoclonal antibody (KCNQ2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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Slo1/BKAlpha maxi-K+ channel scFv [L6/60]

Plasmid

#199421

Purpose

Mammalian expression of Slo1/BKAlpha maxi-K+ channel scFv. Derived from hybridoma L6/60.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targetingSlo1/BKAlpha maxi-K+ channel (Rat) (Kcnma1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-IP3 receptor, type 1 [L24/19R]

Plasmid

#188171

Purpose

Mammalian Expression Plasmid of anti-IP3 receptor, type 1 (Rat). Derived from hybridoma L24/19

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-IP3 receptor, type 1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Itpr1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-IP3 receptor, type 1 [L24/21R]

Plasmid

#188167

Purpose

Mammalian Expression Plasmid of anti-IP3 receptor, type 1 (Rat). Derived from hybridoma L24/21

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-IP3 receptor, type 1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Itpr1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W

Plasmid

#67979

Purpose

Cas9 activity reporter (control) with BFP and GFP.

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKBFP2AGFP cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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Anti-SAP102 [N19/2-rat IgG2a] - Chimeric

Plasmid

#227076

Purpose

Mammalian expression plasmid of anti-SAP102 (Rattus norvegicus) rat IgG2a R-mAb. Derived from hybridoma N19/2.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-SAP102 (Rattus norvegicus) recombinant mouse monoclonal antibody. (Dlg3 Chimera: M. musculus (mouse)/R. norvegicus (rat))

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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Anti-Kv7.1/KCNQ1 K+ channel [N37A/10R]

Plasmid

#177539

Purpose

Mammalian Expression Plasmid of anti-Kv7.1/KCNQ1 K+ channel (Human). Derived from hybridoma N37A/10.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Kv7.1/KCNQ1 K+ channel (Homo sapiens) recombinant mouse monoclonal antibody (KCNQ1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pKLV2.2-h7SKgRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

Plasmid

#72666

Purpose

Lentiviral dual CRISPR gRNA expression vector (human 7SK and human U6 promoters)

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

h7SKgRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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Anti-Kv2.1 K+ channel (external) [K39/25R-2b]

Plasmid

#206625

Purpose

Mammalian Expression Plasmid of anti-Kv2.1 K+ channel (external) (Human). Derived from hybridoma K39/25-2b.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Kv2.1 K+ channel (external) (Homo sapiens) recombinant Mouse monoclonal antibody (KCNB1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pKLV2.2-mU6gRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

Plasmid

#72667

Purpose

Lentiviral dual CRISPR gRNA expression vector (mouse U6 and human U6 promoters)

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

mU6gRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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Lenti-CMV-MLH1dn-IRES-puroR

Plasmid

#207354

Purpose

Lentiviral transfer plasmid containing CMV-driven expression cassette encoding MLH1dn for prime editing (PE4 and PE5 systems).

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

MutL homolog 1 (MLH1 Human)

Use

Lentiviral

Mutation

Δ754-756 dominant negative mutant

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-BsdCas9-W

Plasmid

#67978

Purpose

Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the N-terminus

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

EF1a-Cas9 cassette, WPRE

Use

CRISPR and Lentiviral

Tags

Cas9 fused with Flag-tag and a NLS at the N termi…

Promoter

EF1a

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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Anti-GluA2/GluR2 glutamate receptor [L21/32R-1]

Plasmid

#206693

Purpose

Mammalian Expression Plasmid of anti-GluA2/GluR2 glutamate receptor (Rat). Derived from hybridoma L21/32-1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GluA2/GluR2 glutamate receptor (Rattus norvegicus) recombinant Mouse monoclonal antibody (Gria2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Empty)-PGKmCherry2ABFP-W

Plasmid

#67985

Purpose

Cas9 activity reporter (control) with mCherry and BFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKmCherryABFP cassette, WPRE

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE, modified BFP

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP

Plasmid

#67991

Purpose

CRISPR gRNA expression vector with an improved l scaffold and puro/BFP markers without WPRE

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette with an improved scaffold, PGKpuro2ABFP cassette

Use

CRISPR and Lentiviral

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(BbsI)-PGKpuro2AmAG-W

Plasmid

#67976

Purpose

CRISPR gRNA expression vector with an improved scaffold and puro/mAG markers

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKpuro2AmAG cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCF806-shABC2

Plasmid

#186714

Purpose

Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC2 = shARAF.169-shBRAF.1296-shCRAF.387). The shABC2 set is better than shABC1.

Depositor

Christof Fellmann

Article

Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

Insert

ARAF, BRAF, CRAF (BRAF, RAF1, ARAF Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCF806-shABC1

Plasmid

#186713

Purpose

Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC1 = shARAF.381-shBRAF.2015-shCRAF.2446). The shABC2 set is better than shABC1.

Depositor

Christof Fellmann

Article

Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

Insert

ARAF, BRAF, CRAF (BRAF, RAF1, ARAF Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(BbsI)-PGKpuro2AZsG-W

Plasmid

#67975

Purpose

CRISPR gRNA expression vector with an improved scaffold and puro/ZsG markers

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKpuro2AZsG cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge