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Rae1-pDONR221

Plasmid

#124240

Purpose

Gateway entry clone of Drosophila Rae1 cDNA.

Depositor

Peter Vilmos

Article

Kristo et al Biochim Biophys Acta Mol Cell Res. 2017

Insert

Drosophila Rae1 (Rae1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAP2

Plasmid

#41755

Depositor

Jeff Gelles

Article

Martin et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

kinesin heavy chain (Khc E. coli, Fly)

Use

Tags

His6 and bccp

Expression

Bacterial

Mutation

residues 1-401; C45S C338S K349C C451S

Promoter

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
KHBD00020

Plasmid

#34182

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG1429 (Mef2 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
ninaE-stops myc

Plasmid

#26856

Depositor

Craig Montell

Article

Wang et al Neuron. 2008 Jan 10. 57(1):56-68.

Insert

Slow Termination of Phototransduction (stops Fly)

Use

Tags

myc

Expression

Insect

Mutation

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
KHBD00065

Plasmid

#34224

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG10318 (NC2alpha Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-taiman

Plasmid

#17584

Depositor

Denise Montell

Article

Bai et al Cell. 2000 Dec 22. 103(7):1047-58.

Insert

taiman (tai Fly)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only
pCFJ1259

Plasmid

#44479

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, peel-1, [4-3] Gateway

Use

Gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pUASP YFP Rab11 DN

Plasmid

#37712

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab11 (Rab11 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

S25N (dominant negative)

Promoter

GAL4

Available since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
KHBD00532

Plasmid

#39630

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG3629 (Dll Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Aug. 21, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00219

Plasmid

#34367

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG9015 (en Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab8 CA

Plasmid

#37707

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab8 (Rab8 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Q67L (constitutive active)

Promoter

GAL4

Available since

Nov. 16, 2012

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab8 DN

Plasmid

#37706

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab8 (Rab8 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

T22N (dominant negative)

Promoter

GAL4

Available since

Nov. 16, 2012

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab30 CA

Plasmid

#37737

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab30 (Rab30 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Q66L (constitutive active)

Promoter

GAL4

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
Dscam1_7.27.25 EC10-Fc-His

Plasmid

#72188

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.25 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.25

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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Dscam1_7.27.25 EC10-AP-His

Plasmid

#72062

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.25 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.25

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pUASt-SIK3 S563A

Plasmid

#52976

Purpose

insect expression of SIK3 S563A PKA phosphorylation defective mutant

Depositor

Marc Montminy, Biao Wang

Article

Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

Insert

SIK3 (Sik3 Fly)

Use

Tags

HA

Expression

Insect

Mutation

S563A PKA phosphorylation defective

Promoter

Hsp70

Available since

May 5, 2014

Availability

Academic Institutions and Nonprofits only
pCFJ1200

Plasmid

#44485

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, PuroR, peel-1, MCS

Use

Multiple cloning site vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pUAST-attB-yki:V5-S111A

Plasmid

#85619

Purpose

Expresses V5 tagged mutant yki in Drosophila.

Depositor

Kenneth Irvine

Article

Oh et al Oncogene. 2009 Apr 30;28(17):1916-27. do

Insert

yki-V5-S111A

Use

Tags

V5

Expression

Insect

Mutation

Promoter

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pUAST-attB-yki:V5-S250A

Plasmid

#85618

Purpose

Expresses V5 tagged mutant yki in Drosophila.

Depositor

Kenneth Irvine

Article

Oh et al Oncogene. 2009 Apr 30;28(17):1916-27. do

Insert

yki-V5-S250A

Use

Tags

V5

Expression

Insect

Mutation

Promoter

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmHPat

Plasmid

#25029

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

HPat (Patr-1 Fly)

Use

Drosophila expression

Tags

EGFP

Expression

Mutation

Promoter

Available since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge