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AByF

Plasmid

#111084

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Publication

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Inserts

Y homology arm left
gypsy insulator
3xP3 promoter
tdTomato
SV40 3'UTR
CTCF insulator
Y homology arm right

Expression

Insect

Available since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGex4T1-JET

Plasmid

#136245

Purpose

Protein expression of Jetlag

Depositor

Aziz Sancar

Publication

Ozturk et al J Biol Chem. 2014 Feb 21;289(8):4634-42.

Insert

Jetlag

Tags

GST

Expression

Bacterial

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pET45b-tsr(B/X) (6X-His-Dm-Cofilin)

Plasmid

#128278

Purpose

Expresses Drosophila melanogaster Cofilin (Twinstar) in bacterial cell culture

Depositor

Anna Sokac

Publication

Figard et al Cell Rep. 2019 Mar 26;26(13):3493-3501.e

Insert

twinstar (tsr) (tsr Fly)

Tags

6X Histidine tag

Expression

Bacterial

Mutation

Met1 changed to Pro1; Cys77 silent basepair mutat…

Promoter

T7

Available since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pTIGER_tdTomato::3xFLAG::dCrk

Plasmid

#131138

Purpose

UASp construct for over-expression of Drosophila Crk with N-terminal tandem Tomato and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.

Depositor

Mark Peifer

Publication

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

Crk (Crk Fly)

Tags

3XFLAG and tdTomato

Expression

Insect

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCFD3-Act5c

Plasmid

#130278

Purpose

Drosophila expression of sgRNA that targets Actin5c CDS close to stop codon. Positive control sgRNA for CRISPaint knock-ins in cultured cells.

Depositor

Norbert Perrimon

Publication

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

pCFD3-Act5c (Act5C Fly)

Use

CRISPR

Expression

Insect

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Nup98-pDONR221

Plasmid

#124241

Purpose

Gateway entry clone of Drosophila Nup98-96 cDNA.

Depositor

Peter Vilmos

Publication

Kristo et al Biochim Biophys Acta Mol Cell Res. 2017

Insert

Drosophila Nup98-96 (Nup98-96 Fly)

Expression

Insect

Available since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

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Act5C-pDONR221

Plasmid

#124242

Purpose

Gateway entry clone of Drosophila Actin5C cDNA.

Depositor

Peter Vilmos

Insert

Drosophila Actin 5C (Act5C Fly)

Expression

Insect

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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Rae1-pDONR221

Plasmid

#124240

Purpose

Gateway entry clone of Drosophila Rae1 cDNA.

Depositor

Peter Vilmos

Publication

Kristo et al Biochim Biophys Acta Mol Cell Res. 2017

Insert

Drosophila Rae1 (Rae1 Fly)

Expression

Insect

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-Hemmar-FLP(D)

Plasmid

#119895

Purpose

FLP(D) with a hsp70 promoter

Depositor

Rita Sousa-Nunes

Publication

Chai et al Elife. 2018 Nov 27;7. pii: 38393. doi: 1

Insert

FLP(D)

Use

Drosophila expression

Mutation

mutated form of FLP, which at position 5 contains…

Available since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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Dscam1_7.27.25 EC10-Fc-His

Plasmid

#72188

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.25 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Publication

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.25

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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gRNA[Sxl].1026B

Plasmid

#112688

Purpose

express gRNA targeting Sxl under dU6-3 promoter

Depositor

Omar Akbari

Publication

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

U6.3-gRNA[Sxl] (Sxl Fly)

Use

CRISPR

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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gRNA[bTub].1026C

Plasmid

#112691

Purpose

express gRNA targeting bTub under dU6-3 promoter

Depositor

Omar Akbari

Publication

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

U6.3-gRNA[bTub]

Use

CRISPR

Promoter

dU6-3

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTGC-U6.2

Plasmid

#112809

Purpose

PCR template vector for amplifying gRNAcore-pU6.3 promoter fragment

Depositor

Chun Han

Publication

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Insert

U6:2-gRNAcore

Expression

Bacterial

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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His Puc (aa 1-393)

Plasmid

#52295

Purpose

Bacterial expression of His tagged Drosophila Puc fragment

Depositor

Brenda Schulman

Publication

Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

Insert

Puc (puc Fly)

Tags

His

Expression

Bacterial

Promoter

T7

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only
pFastBac Dual PNG-FLAG PLU-HIS

Plasmid

#113173

Purpose

Baculovirus expression of PNG and PLU

Depositor

Terry Orr-Weaver

Publication

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

PNG, PLU

Use

Baculovirus

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pGex4t2 with full-length SUUR

Plasmid

#112990

Purpose

Expresses fusion of GST and full-length Drosophila SUUR

Depositor

Terry Orr-Weaver

Publication

Nordman et al Cell Rep. 2014 Nov 6;9(3):841-9. doi: 10

Insert

SuUR (SuUR Fly)

Expression

Bacterial

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCaSpeR eGFP-dhd

Plasmid

#113004

Purpose

P element vector for transposon insertion into Drosophila genome

Depositor

Terry Orr-Weaver

Insert

dhd (dhd Fly)

Use

P insertion vector

Tags

eGFP

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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mr in pUASP

Plasmid

#112981

Purpose

P element vector for transposon insertion of mr (APC2) into Drosophila genome

Depositor

Terry Orr-Weaver

Publication

Kashevsky et al Proc Natl Acad Sci U S A. 2002 Aug 20;99

Insert

mr (APC2) (mr Fly)

Use

P insertion vector

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pMAL c2x Tral FL

Plasmid

#113012

Purpose

For bacterial expression of MBP fusion of full-length Drosophila Tral WT

Depositor

Terry Orr-Weaver

Publication

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

full length tral (tral Fly)

Expression

Bacterial

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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CaSpeR dhd 3X FLAG

Plasmid

#113002

Purpose

P element vector for transposon insertion of Flag-tagged Drosophila dhd into Drosophila genome

Depositor

Terry Orr-Weaver

Insert

dhd (dhd Fly)

Use

P insertion vector

Tags

FLAG

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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