We narrowed to 1,269 results for: MS2

1. pSX2739

   Plasmid

   #200765

   Purpose

   Expresses mKate2-tag fused CDC-42 with 8xMS2(V1) stem loops in C. elegans, and the tissue-specific promoter col-19 is used.

   Depositor

   Suhong Xu

   Article

   Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

   Insert

   8xMS2
   

   Tags

   mKate2

   Expression

   Worm

   Promoter

   col-19

   Available Since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. SuperNova-N1

   Plasmid

   #131853

   Purpose

   Expresses SuperNova with C-terminal Myc-tag and N-terminal multiple cloning site

   Depositor

   Geert van den Bogaart

   Article

   Paardekooper et al Int J Mol Sci. 2019 Aug 25;20(17). pii:

   Insert

   SuperNova
   

   Tags

   Myc tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. COX8-SuperNova

   Plasmid

   #131856

   Purpose

   Expresses SuperNova fused to mitochrondrial protein COX8A

   Depositor

   Geert van den Bogaart

   Article

   Paardekooper et al Int J Mol Sci. 2019 Aug 25;20(17). pii:

   Insert

   SuperNova
   

   Tags

   Cox8A and Myc tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTK095

   Plasmid

   #59560

   Purpose

   SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4 8xMS2

   Depositor

   Ron Weiss

   Article

   Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

   Insert

   SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4 8xMS2
   

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBluescript JTV-1

   Plasmid

   #16473

   Depositor

   Bert Vogelstein

   Article

   Nicolaides et al Genomics. 1995 Sep 20. 29(2):329-34.

   Insert

   JTV-1 (AIMP2 Human)
   

   Expression

   Bacterial

   Available Since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

6. SuperNova-TGON3

   Plasmid

   #131859

   Purpose

   Expresses NuperNova fused to trans-Golgi protein TGON3

   Depositor

   Geert van den Bogaart

   Article

   Paardekooper et al Int J Mol Sci. 2019 Aug 25;20(17). pii:

   Insert

   SuperNova
   

   Tags

   TGON3 (rat)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. VAMP8-SuperNova

   Plasmid

   #131855

   Purpose

   Expresses SuperNova fused to endosomal SNARE VAMP8

   Depositor

   Geert van den Bogaart

   Article

   Paardekooper et al Int J Mol Sci. 2019 Aug 25;20(17). pii:

   Insert

   SuperNova
   

   Tags

   Myc-tag and VAMP8 (mouse sequence)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-scFvGCN4sfGFP-VP64-GB1

   Plasmid

   #141417

   Purpose

   Express scFvGCN4-sfGFP-VP64 fusion protein for dCas9-SunTag mediated targeted gene activation

   Depositor

   Izuho Hatada

   Article

   Morita et al Int J Mol Sci. 2020 Feb 25;21(5). pii: i

   Insert

   scFvGCN4sfGFP-VP64-GB1
   

   Tags

   HA and sfGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Calreticulin-SuperNova-KDEL

   Plasmid

   #131857

   Purpose

   Expresses SuperNova fused to ER protein calreticulin and KDEL ER-retention signal

   Depositor

   Geert van den Bogaart

   Article

   Paardekooper et al Int J Mol Sci. 2019 Aug 25;20(17). pii:

   Insert

   SuperNova
   

   Tags

   KDEL ER-retention signal and Signal sequence of h…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. NLS-SuperNova

    Plasmid

    #131858

    Purpose

    Expresses SuperNova fused to nuclear localization signal (NLS)

    Depositor

    Geert van den Bogaart

    Article

    Paardekooper et al Int J Mol Sci. 2019 Aug 25;20(17). pii:

    Insert

    SuperNova
    

    Tags

    Myc tag and c-myc nuclear localization signal (NL…

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-TET3G-T2A-Puro

    Plasmid

    #158067

    Purpose

    Encodes the Tet3G transactivator and puromycin resistance.

    Depositor

    Adolfo Rivero-Muller

    Article

    Michalec-Wawiorka et al Int J Mol Sci. 2021 May 14;22(10). pii:

    Insert

    pCAG-TET3G-T2A-Puro
    

    Use

    AAV

    Tags

    puromycin reistance gene

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. GWF297

    Plasmid

    #133612

    Purpose

    TET3G-2xMS2(wt+f6), NLS-MCP-ANR-ANR-P2a-BFP

    Depositor

    Dustin Maly

    Article

    Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

    Insert

    TET3G-2xMS2(wt+f6), MCP-ANR-ANR, BFP
    

    Expression

    Mammalian

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. fADL-1e-flag-linker-p3

    Plasmid

    #139441

    Purpose

    For cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage.

    Depositor

    Alexander Gabibov

    Article

    Ishina et al Int J Mol Sci. 2020 Aug 29;21(17). pii:

    Insert

    linker with pelB-leader, cloning site, flag-tag and p3
    

    Expression

    Bacterial

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. fADL-1e-HA-linker-p3

    Plasmid

    #139440

    Purpose

    For cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage

    Depositor

    Alexander Gabibov

    Article

    Ishina et al Int J Mol Sci. 2020 Aug 29;21(17). pii:

    Insert

    linker with pelB-leader, cloning site, HA-tag and p3
    

    Expression

    Bacterial

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRE3G-EGFP-IRES(Neo)R

    Plasmid

    #158068

    Purpose

    eGFP under the TRE3G promoter, Neo resistance. For cloning GOI

    Depositor

    Adolfo Rivero-Muller

    Article

    Michalec-Wawiorka et al Int J Mol Sci. 2021 May 14;22(10). pii:

    Insert

    eGFP
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. dCas9-10xSunTag-P2A-tagBFP

    Plasmid

    #174141

    Purpose

    Expresses the dCas9-10xSunTag-tagBFP fusion protein for the recruitment of scFv(GCN4)-fused effector domains. A shorter version of the Addgene 60903 plasmid.

    Depositor

    Albert Jeltsch

    Article

    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

    Insert

    dCas9, 10xSunTag, tagBFP
    

    Tags

    HA and tagBFP

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTA-Mob 2.0

    Plasmid

    #149662

    Purpose

    Conjugation helper plasmid for use in cis (self-transfer) and trans (transfer of plasmids with RK2/RP4 origin of transfer) mobilization to prokaryotic and eukaryotic cells.

    Depositor

    Bogumil Karas

    Article

    Soltysiak et al Int J Mol Sci. 2019 Oct 21;20(20). pii:

    Type

    Empty backbone

    Use

    Synthetic Biology; Conjugation helper plasmid

    Expression

    Bacterial

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMXs-KDM4D

    Plasmid

    #188234

    Purpose

    Express human KDM4D in mammalian cells

    Depositor

    Young Tang

    Article

    Su et al Int J Mol Sci. 2021 Sep 28;22(19). pii:

    Insert

    Lysine demethylase 4D (KDM4D Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAWH-IRP

    Plasmid

    #133896

    Purpose

    Positive control when used with pMS22H-IRE. Expression of Gal4AD-IRP hybrid protein. Homology regions for recombination with pMS22H

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Insert

    IRP
    

    Expression

    Yeast

    Promoter

    T7

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCML 313

    Plasmid

    #98586

    Purpose

    pNYFP + CsrB-2MS2BD. Expresses NYFP control fusion and RNA-2MS2BD fusion in E. coli for TriFC assay (ampR).

    Depositor

    Lydia Contreras

    Article

    Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

    Insert

    csrB
    

    Expression

    Bacterial

    Promoter

    pLacO

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCML 314

    Plasmid

    #98584

    Purpose

    pCsrA-NYFP + CsrB-2MS2BD. Expresses protein-NYFP and RNA-2MS2BD fusions in E. coli for TriFC assay (ampR).

    Depositor

    Lydia Contreras

    Article

    Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

    Inserts

    CsrA
    csrB
    

    Expression

    Bacterial

    Promoter

    pLacO

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCW57.1-MAT2A

    Plasmid

    #100521

    Purpose

    Dox-repressible cDNA expression of MAT2A (dox-off/ tet-off all-in-one vector)

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    MAT2A (MAT2A Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    pTight TRE

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pMXs-KDM4A

    Plasmid

    #188233

    Purpose

    Express human KDM4A in mammalian cells

    Depositor

    Young Tang

    Article

    Su et al Int J Mol Sci. 2021 Sep 28;22(19). pii:

    Insert

    Lysine-specific demethylase 4A (KDM4A Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. LIC-Z-Y-L

    Plasmid

    #153546

    Purpose

    this is signaling incompetent version of LIC-Z where all Tyrosine residues in ITAMs are mutated to leucine

    Depositor

    Katharina Gaus

    Article

    Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

    Insert

    human TCR ζ-Chain (CD247 Human)
    

    Expression

    Mammalian

    Mutation

    with 6 Y in 3 ITAMs mutated to L

    Promoter

    CMV

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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25. scFv-GCN4-DNMT3a(R887E)-DNMT3l

    Plasmid

    #154141

    Purpose

    Expresses a higher specificity variant of scFv-GCN4-DNMT3a-DNMT3l, contains R887E mutation in DNMT3a. To be used with the dCas9-SunTag system for targeted DNA methylation.

    Depositor

    Albert Jeltsch

    Article

    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

    Insert

    scFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
    

    Tags

    HA and sfGFP

    Expression

    Mammalian

    Mutation

    R887E

    Promoter

    SFFV

    Available Since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP

    Plasmid

    #154139

    Purpose

    Expresses the dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP fusion protein for targeted DNA methylation.

    Depositor

    Albert Jeltsch

    Article

    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

    Insert

    dCas9, DNMT3a (catalytic domain), DNMT3l (C-terminal part), tagBFP
    

    Tags

    3x FLAG and tagBFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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27. scFv-GCN4-DNMT3a-DNMT3l

    Plasmid

    #154140

    Purpose

    Expresses the scFv-GCN4-DNMT3a-DNMT3l fusion protein (more details are shown in the vectro map) for targeted DNA methylation. Should be used in a combination with the dCas9-SunTag systems.

    Depositor

    Albert Jeltsch

    Article

    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

    Insert

    scFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
    

    Tags

    HA and sfGFP

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCR2.1_HBB_N

    Plasmid

    #87847

    Purpose

    pCR2.1-based plasmid holding a 309-bp fragment containing the exon-1-exon-2 splice border of normal human β-globin mRNA

    Depositor

    Marina Kleanthous

    Article

    Patsali et al Int J Mol Sci. 2020 Sep 11;21(18). pii:

    Insert

    Homo sapiens hemoglobin subunit beta (HBB), Normal cDNA fragment (Exon1/2) (HBB Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. LIC-Z

    Plasmid

    #153543

    Purpose

    perform light induced optogenetic clustering of TCR ζ-Chain as described in the associated publication

    Depositor

    Katharina Gaus

    Article

    Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

    Insert

    human TCR ζ-Chain (CD247 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    CMV

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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30. LIC-Z-delCry2

    Plasmid

    #153545

    Purpose

    this is the non-clustering version of LIC-Z tagged with mcherry

    Depositor

    Katharina Gaus

    Article

    Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

    Insert

    human TCR ζ-Chain (CD247 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    with Cry2 sequence deleted from LIC-Z

    Promoter

    CMV

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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