We narrowed to 15,742 results for: grna

1. pTBL513 TT sgRNA

   Plasmid

   #126441

   Purpose

   To target a protospacer with TT at the cut site.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   spacer against human genome with TT at cut site
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. St1Cas9_LMD-9_sgRNA_pUC19

   Plasmid

   #110627

   Purpose

   U6-driven sgRNA expression plasmid for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9).

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Insert

   Empty St1Cas9 LMD-9 sgRNA backbone
   

   Use

   CRISPR and Synthetic Biology

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKDsgRNA-fsaA

   Plasmid

   #102286

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting fsaA.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   fsaA gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available Since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKDsgRNA-adhE

   Plasmid

   #102290

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting adhE.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   adhE gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available Since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKDsgRNA-ompT

   Plasmid

   #102291

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting ompT.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   ompT gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available Since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX458-sgRNA_Ago1_4

   Plasmid

   #73536

   Purpose

   Expresses SpCas9, GFP, and sgRNA targeting Ago1

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   AGO1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

7. EMX1*_sgRNA

   Plasmid

   #100558

   Purpose

   Expresses EMX1* sgRNA. Target sequence: (G)GAGTCCGAGCAGAAGAAGAA. Same target sequence as #100555, except with an additional 5' G.

   Depositor

   Dustin Maly

   Article

   Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

   Insert

   EMX1 sgRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKDsgRNA-srlA

   Plasmid

   #89965

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting srlA.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   srlA gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKDsgRNA-rbsK

   Plasmid

   #89963

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rbsK.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   rbsK gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKDsgRNA-yhhT

    Plasmid

    #89958

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting yhhT.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    yhhT gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKDsgRNA-torZ

    Plasmid

    #89956

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting torZ.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    torZ gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKDsgRNA-arsB

    Plasmid

    #89952

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting arsB.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    arsB gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p426_Cas9_gRNA-SAP155c

    Plasmid

    #87390

    Purpose

    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting SAP155c sequence ATGAAAGACAACTATAGGGC in yeast chromosome 6

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting SAP155c
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ADH1, pTyrosine

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p426_Cas9_gRNA-ARS607c

    Plasmid

    #87388

    Purpose

    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS607c sequence CTATTTTTGCTTTCTGCACA in yeast chromosome 6.

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS607c
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ADH1, pTyrosine

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p426_Cas9_gRNA-SAP155b

    Plasmid

    #87389

    Purpose

    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting SAP155b sequence GGTTTTCATACTGGGGCCGC in yeast chromosome 6

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting SAP155b
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ADH1, pTyrosine

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p426_Cas9_gRNA-ARS805a

    Plasmid

    #87393

    Purpose

    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS805a sequence TTATTTGAATGATATTTAGT in yeast chromosome 8.

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS805a
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ADH1, pTyrosine

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p426_Cas9_gRNA-ARS1206a

    Plasmid

    #87398

    Purpose

    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1206a sequence CGAACATTTTTCCATGCGCT in yeast chromosome 12.

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1206a
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ADH1, pTyrosine

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p426_Cas9_gRNA-ARS1414a

    Plasmid

    #87400

    Purpose

    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1414a sequence GCGCCACAGTTTCAAGGGTC in yeast chromosome 14.

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1414a
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ADH1, pTyrosine

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p426_Cas9_gRNA-R-ARS805a

    Plasmid

    #87408

    Purpose

    p426_Cas9_gRNA-ARS805a without ribozyme All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS805a sequence TTATTTGAATGATATTTAGT in yeast chromosome 8.

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS805a
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ADH1, pTyrosine

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p426_Cas9_gRNA-RDS1a

    Plasmid

    #87385

    Purpose

    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting RDS1a sequence ATTCAATACGAAATGTGTGC in yeast chromosome 3

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting RDS1a
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ADH1, pTyrosine

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHU6_gRNA_Ch10-for2

    Plasmid

    #81209

    Purpose

    for targeting Ch10 psuedo gix site in PCDH15 gene (five base pairs from the cas9 site and 3' of gix site)

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    hU6 expression of gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. GK gRNA (BRDN0001146383)

    Plasmid

    #76329

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human GK

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    GK (GK Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. GK gRNA (BRDN0001148115)

    Plasmid

    #76326

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human GK

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    GK (GK Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. GK gRNA (BRDN0001145307)

    Plasmid

    #76327

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human GK

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    GK (GK Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. GK gRNA (BRDN0001146936)

    Plasmid

    #76328

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human GK

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    GK (GK Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pT7-gRNA:Asip

    Plasmid

    #60968

    Purpose

    Expression vector of rat Asip guide RNA

    Depositor

    Tomoji Mashimo

    Article

    Yoshimi et al Nat Commun. 2014 Jun 26;5:4240. doi: 10.

    Insert

    Agouti signaling protein gRNA (Asip Rat)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pT7-gRNA:kit-1

    Plasmid

    #60969

    Purpose

    Expression vector of rat Kit-1 guide RNA

    Depositor

    Tomoji Mashimo

    Article

    Yoshimi et al Nat Commun. 2014 Jun 26;5:4240. doi: 10.

    Insert

    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog gRNA (Kit Rat)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pT7-gRNA:kit-2-1

    Plasmid

    #60970

    Purpose

    Expression vector of rat Kit-2-1 guide RNA

    Depositor

    Tomoji Mashimo

    Article

    Yoshimi et al Nat Commun. 2014 Jun 26;5:4240. doi: 10.

    Insert

    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog gRNA (Kit Rat)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pT7-gRNA:kit-2-2

    Plasmid

    #60971

    Purpose

    Expression vector of rat Kit-2-2 guide RNA

    Depositor

    Tomoji Mashimo

    Article

    Yoshimi et al Nat Commun. 2014 Jun 26;5:4240. doi: 10.

    Insert

    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog gRNA (Kit Rat)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

30. pU6-pegRNA-GG-acceptor

    Plasmid

    #132777

    Purpose

    Prime editing in mammalian cells

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    Exchangeable cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge