We narrowed to 10,893 results for: ENA;

1. pETDuet-solo-NHis6-NocO-E94A

   Plasmid

   #240401

   Purpose

   expresses N-His tagged NocO E94A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid

   Depositor

   Emily Balskus

   Article

   Wang et al Biochemistry. 2025 May 20;64(10):2173-21

   Insert

   nocO
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   M1 of native sequence not included in insert, Glu…

   Promoter

   T7

   Available Since

   July 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21a(+)_co_L-HicDH

   Plasmid

   #185453

   Purpose

   CDS of L-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 in pET21a(+).

   Depositor

   Wolfgang Kroutil

   Article

   Jurkas et al Eng Microbiol. 2021 Nov 26;2(1):100008.

   Insert

   L-HicDH from Lactobacillus confusus DSM 20196
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pST_LEA1_APHAV (pBS0335)

   Plasmid

   #185180

   Purpose

   For the mammalian expression of the nematode (Aphelenchus avenae) protein LEA1_APHAV. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   LEA1_APHAV
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. LEA1_APHAV_Chakrabortee pSecTag (pBS0254)

   Plasmid

   #185143

   Purpose

   For the mammalian expression of the nematode (Aphelenchus avenae) protein LEA1_APHAV. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   LEA1_APHAV
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. DCT.VV.Orf269

   Plasmid

   #12957

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jon Yewdell, Jack Bennink

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf269
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 29, 2006

   Availability

   Academic Institutions and Nonprofits only

6. dCas9-VPR_P2A_mCherry

   Plasmid

   #154193

   Purpose

   Lentiviral expression plasmid encoding dCas9-VPR and a P2A linked mCherry marker

   Depositor

   Anna Obenauf

   Article

   Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

   Insert

   sp-dCas9-VPR_P2A_mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   EF1as

   Available Since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Dual-sgRNA_hU6-mU6

   Plasmid

   #154194

   Purpose

   Lentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.

   Depositor

   Anna Obenauf

   Article

   Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGG-B1

   Plasmid

   #78504

   Purpose

   Trans plasmid for production of recombinant AAV-B1 vectors. Plasmid backbone may yield higher titers than pAR-B1 and encodes AAV2 Rep and AAV-B1 capsid

   Depositor

   Miguel Sena-Esteves

   Article

   Choudhury et al Mol Ther. 2016 Apr 27. doi: 10.1038/mt.2

   Inserts

   AAV2 Rep
   AAV-B1 capsid
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIRES-hrGFP II-TET1

   Plasmid

   #83568

   Purpose

   Expresses TET1 in mammalian cells

   Depositor

   Jean-Pierre Issa

   Article

   Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

   Insert

   Tet Methylcytosine Dioxygenase 1 (TET1 Human)
   

   Use

   Tags

   3X FLAG tag and hr GFP II

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIRES-hrGFP II-TET1_CD

    Plasmid

    #83570

    Purpose

    Expresses the catalytic domain of TET1 in mammalian cells

    Depositor

    Jean-Pierre Issa

    Article

    Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

    Insert

    Tet Methylcytosine Dioxygenase 1 (TET1 Human)
    

    Use

    Tags

    3X FLAG tag and hr GFP II

    Expression

    Mammalian

    Mutation

    TET1 amino acids 1-1417 deleted

    Promoter

    CMV

    Available Since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA4/TO/GFP-ZNF598

    Plasmid

    #141191

    Purpose

    Expresses GFP-ZNF598 in mammalian cells, can be used to make inducible cell line

    Depositor

    Simon Bekker-Jensen

    Article

    Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

    Insert

    ZNF598 (ZNF598 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAG876 pDisplay-pFAST

    Plasmid

    #172868

    Purpose

    expresses pFAST at the surface of mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

    Insert

    pFAST-PDGFR
    

    Use

    Tags

    IgK leader sequence (N terminal on insert) and My…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGG-9.47_VP1,3 #2515

    Plasmid

    #74237

    Purpose

    Modified plasmid with a different backbone that yields higher of AAV-AS vectors. Encodes VP1 and VP3 of AAV9.47

    Depositor

    Miguel Sena-Esteves

    Article

    Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2

    Inserts

    AAV2 Rep
    AAV9.47 VP1
    AAV9.47 VP3
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-WT-VLCAD-FLAG

    Plasmid

    #55823

    Purpose

    Expresses WT-VLCAD in mammalian cells

    Depositor

    Yoshimi Homma

    Article

    Kabuyama et al Am J Physiol Cell Physiol. 2010 Jan;298(

    Insert

    Very long-chain specific acyl-CoA dehydrogenase (ACADVL Human)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. Ana GvpC-SpyTag-His in pET28a

    Plasmid

    #85737

    Purpose

    Expresses His-tagged GvpC from Anabaena flos-aquae fused to SpyTag in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

    Insert

    Gas vesicle protein C (fused to SpyTag and His-tagged)
    

    Use

    Tags

    His-tag and SpyTag

    Expression

    Bacterial

    Mutation

    Added an extra glycine after the methionine at th…

    Promoter

    T7

    Available Since

    March 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21b-LOV-ipaA

    Plasmid

    #40236

    Depositor

    Brian Kuhlman

    Article

    Lungu et al Chem Biol. 2012 Apr 20;19(4):507-17.

    Insert

    LOV-ipaA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Jα helix region hybridized (AsLOV2 aa 537-546/ipa…

    Promoter

    T7

    Available Since

    Sept. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pGEX6P1-Ubl-sr1(SIRPT1)

    Plasmid

    #75298

    Purpose

    Expresses Sacsin Ubl-sr1 (SIRPT1 repeat) domain (1-339) in fusion with GST (bacterial)

    Depositor

    Kalle Gehring

    Article

    Menade et al J Biol Chem. 2018 Aug 17;293(33):12832-1

    Insert

    Human Sacsin 1-339
    

    Use

    Tags

    GST-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDF1-ADAMTS18 Wt

    Plasmid

    #29552

    Depositor

    Yardena Samuels

    Article

    Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

    Insert

    ADAMTS18 (ADAMTS18 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Wild type

    Promoter

    Available Since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pAR-9.47_VP1,3

    Plasmid

    #74240

    Purpose

    Original plasmid described in Choudhury et al. 2015 Mol Ther. for production of AAV-AS vectors. Encodes VP1 and VP3 of AAV9.47

    Depositor

    Miguel Sena-Esteves

    Article

    Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2

    Inserts

    AAV2 Rep
    AAV9.47 VP1
    AAV9.47 VP3
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. WT N-His Ana GvpC in pET28a

    Plasmid

    #153294

    Purpose

    Expresses N-terminal His-tagged GvpC from Anabaena flos-aquae in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    Gas vesicle protein C (N-terminal his-tagged)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Added a hexahistidine tag after the methionine at…

    Promoter

    T7

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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