We narrowed to 17,401 results for: Eomes

1. pLEX-FHH-Empty Vector-IRES-Puro

   Plasmid

   #120568

   Purpose

   For cloning genes with an N-terminal TAP tag [Flag-HA-6xHIS]

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Flag-HA-6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRL278

   Plasmid

   #70256

   Purpose

   sacB plasmid with polylinker; kanamycin/neomycin resistance: used as a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion seq.

   Depositor

   Peter Wolk

   Article

   Cai et al J Bacteriol. 1990 Jun;172(6):3138-45.

   Type

   Empty backbone

   Use

   Conditionally lethal

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBA2675

   Plasmid

   #189997

   Purpose

   Inducible expression of FBP75 (1–200)-NLS-VhhGFP4, integrate at 177 bp, phleomycin marker (deGradFP for nuclear proteins)

   Depositor

   Bungo Akiyoshi

   Article

   Ishii et al Wellcome Open Res. 2022 Jun 23;7:175. do

   Inserts

   FBP75
   VhhGFP4
   

   Use

   Expression vector for trypanosoma brucei

   Tags

   NLS

   Expression

   Mutation

   N-terminal 600 bp of FBP75

   Promoter

   Available since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-EF1a-LIC-V5

   Plasmid

   #120248

   Purpose

   For LIC cloning with C-terminal V5 tag

   Depositor

   Larry Gerace

   Article

   Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFTK094-LM-AMA002.0

   Plasmid

   #171366

   Purpose

   Entry Vector compatible, fungal replicating, shuttle backbone vector from the Fungal Modular Cloning ToolKit.

   Depositor

   Arnold Driessen

   Article

   Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   n/a

   Promoter

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-FLAG-APEX2-GFP

   Plasmid

   #175757

   Purpose

   Lentiviral vector expressing N-terminally tagged APEX2-FLAG-GFP (Control for APEX experiments)

   Depositor

   Wade Harper

   Article

   Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. p3E-TurboID

   Plasmid

   #166570

   Purpose

   Multisite Gateway 3' entry clone for fusions onto the N-terminus of TurboID. Parton lab clone JSS

   Depositor

   Rob Parton

   Article

   Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

   Insert

   TurboID
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSET-His-APEX2

   Plasmid

   #201471

   Purpose

   Recombinant soluble APEX2 for SLAPSHOT labeling

   Depositor

   Yuh-Nung Jan

   Article

   Tuomivaara et al Commun Biol. 2024 Aug 29;7(1):1060. doi:

   Insert

   APEX2
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-sgNeo2/Cre

   Plasmid

   #89652

   Purpose

   Expresses a Neomycin-targeting gRNA and Cre-recombinase

   Depositor

   Monte Winslow

   Article

   Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

   Insert

   gRNA targeting Neomycin
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-EF1a-V5-LIC

    Plasmid

    #120247

    Purpose

    For LIC cloning with N-terminal V5 tag

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLIK 3XFLAG-EGFP-NLS hygro

    Plasmid

    #87781

    Purpose

    Inducible expression of EGFP with a C-terminal NLS sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pME-TurboID-NS

    Plasmid

    #166566

    Purpose

    Multisite Gateway middle entry clone encoding TurboID. Parton lab clone JRP

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    TurboID
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. hTMEM115-TEV-3xHA-P2A-mScarlet HDR template

    Plasmid

    #214998

    Purpose

    Endogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    hTMEM115-TEV-3xHA-P2A-mScarlet HDR template (TMEM115 Human)
    

    Use

    Hdr template

    Tags

    3xHA

    Expression

    Mutation

    Promoter

    None

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. hTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template

    Plasmid

    #214999

    Purpose

    Endogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mNG for cell sorting.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    hTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template (TMEM115 Human)
    

    Use

    Hdr template

    Tags

    3xHA

    Expression

    Mutation

    Promoter

    None

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKPY514

    Plasmid

    #62598

    Purpose

    Encodes Thr251Gly-EcPheRS Under IPTG-Inducible (PT5) Control

    Depositor

    David Tirrell

    Article

    Yuet et al Proc Natl Acad Sci U S A. 2015 Feb 17. p

    Insert

    Thr251Gly-EcPheRS
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Thr251Gly

    Promoter

    T5

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE pGK hNLRP3-mNG

    Plasmid

    #218643

    Purpose

    Expresses NLRP3 tagged with mNeonGreen in mammalian cells (lentiviral); driven by the weak pGK promoter with blast selection

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (NLRP3 Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    pGK

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pINTO-N-SA

    Plasmid

    #61723

    Purpose

    Inducible expression of proteins fused to an N-terminal monomeric streptavidin tag (mSA)

    Depositor

    Roberto Bonasio

    Article

    Beck et al J Proteome Res. 2014 Dec 5;13(12):6135-4

    Type

    Empty backbone

    Use

    Tags

    mSA

    Expression

    Mammalian

    Mutation

    Promoter

    T-REx (CMV + 2xTetO2)

    Available since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT3TS-TurboID-EGFP

    Plasmid

    #163846

    Purpose

    In vitro transcription of TurboID-EGFP. Parton lab clone JRS

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    TurboID
    

    Use

    In vitro transcription of rna

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    Sept. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28-OmpT

    Plasmid

    #68862

    Purpose

    construct for expression of OmpT protease in the pET28a plasmid using the restriction sites NcoI and EcoRI.

    Depositor

    Neil Kelleher

    Article

    Wu et al Nat Methods. 2012 Jun 17;9(8):822-4. doi

    Insert

    OmpT (ompT )
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGBT-RP2-1

    Plasmid

    #31828

    Depositor

    Stephen Ekker

    Article

    Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    GFP GM2 variant and mRFP

    Expression

    Mutation

    Promoter

    beta Actin

    Available since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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